LOCUS OGT-pGEn2-DEST 8370 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 6998..7539 /note="WPRE" misc_recomb 6960..6982 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(7546..7760) /note="BGH pA" misc_feature complement(7848..7977) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" misc_feature 3868..3890 /note="TEV site" source 3891..6953 /dnas_title="OGT-pGEn2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:22921 IMAGE:4865031" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_15" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3891..6953 /dnas_title="OGT" /gene="OGT" /gene_synonym="FLJ23071" /gene_synonym="HRNT1" /gene_synonym="MGC22921" /gene_synonym="O-GLCNAC" /db_xref="GeneID:8473" /db_xref="HGNC:8127" /db_xref="MIM:300255" CDS 3891..6953 /dnas_title="O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)" /gene="OGT" /gene_synonym="FLJ23071" /gene_synonym="HRNT1" /gene_synonym="MGC22921" /gene_synonym="O-GLCNAC" /codon_start=1 /product="O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)" /protein_id="AAH14434.1" /db_xref="GI:15680175" /db_xref="GeneID:8473" /db_xref="HGNC:8127" /db_xref="MIM:300255" /translation="MASSVGNVADSTGLAELAHREYQAGDFEAAERHCMQLWRQEPDN TGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHY RHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDLGNLLKAL GRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDPNFLDAY INLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAI ELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIE EAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMG NTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIASYRTALKLKP DFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPHHSMLYPLSHG FRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFGNHPTSHLMQS IPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKAADRIHQDGIH ILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQETSPAEVAEQY SEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPD VKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQITINGFSISNGL ATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPSTLQMWANILK RVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRRGQLADVCLDT PLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKNRQEYEDIAVK LGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAGNKPDHMIKPV EVTESA" misc_feature 3891..6953 /note="OGT coding region" vector join(6968..8370,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..6967 /source="OGT-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc gagttggcac 3901 atcgagaata tcaggcagga gattttgagg cagctgagag acactgcatg cagctctgga 3961 gacaagagcc agacaatact ggtgtgcttt tattactttc atctatacac ttccagtgtc 4021 gaaggctgga cagatctgct cactttagca ctctggcaat taaacagaac ccccttctgg 4081 cagaagctta ttcgaatttg gggaatgtgt acaaggaaag agggcagttg caggaggcaa 4141 ttgagcatta tcgacatgca ttgcgtctca aacctgattt catcgatggt tatattaacc 4201 tggcagccgc cttggtagca gcgggtgaca tggaaggggc agtacaagct tacgtctctg 4261 ctcttcagta caatcctgat ttgtactgtg ttcgcagtga cctggggaac ctgctcaaag 4321 ccctgggtcg cttggaagaa gccaaggcat gttatttgaa agcaattgag acgcaaccga 4381 actttgcagt agcttggagt aatcttggct gtgttttcaa tgcacaaggg gaaatttggc 4441 ttgcaattca tcactttgaa aaggctgtca cccttgaccc aaactttctg gatgcttata 4501 tcaatttagg aaatgtcttg aaagaggcac gcatttttga cagagctgtg gcagcttatc 4561 ttcgtgccct aagtttgagt ccaaatcacg cagtggtgca cggcaacctg gcttgtgtat 4621 actatgagca aggcctgata gatctggcaa tagacaccta caggcgggct atcgaactac 4681 aaccacattt ccctgatgct tactgcaacc tagccaatgc tctcaaagag aagggcagtg 4741 ttgctgaagc agaagattgt tataatacag ctctccgtct gtgtcccacc catgcagact 4801 ctctgaataa cctagccaat atcaaacgag aacagggaaa cattgaagag gcagttcgct 4861 tgtatcgtaa agcattagaa gtcttcccag agtttgctgc tgcccattca aatttagcaa 4921 gtgtactgca gcagcaggga aaactgcagg aagctctgat gcattataag gaggctattc 4981 gaatcagtcc tacctttgct gatgcctact ctaatatggg aaacactcta aaggagatgc 5041 aggatgttca gggagccttg cagtgttata cgcgtgccat ccaaattaat cctgcatttg 5101 cagatgcaca tagcaatctg gcttccattc ataaggattc agggaatatt ccagaagcca 5161 tagcttctta ccgcacggct ctgaaactta agcctgattt tcctgatgct tattgtaact 5221 tggctcattg cctgcagatt gtctgtgatt ggacagacta tgatgagcga atgaagaagt 5281 tggtcagtat tgtggctgac cagttagaga agaataggtt gccttctgtg catcctcatc 5341 atagtatgct atatcctctt tctcatggct tcaggaaggc tattgctgag aggcacggca 5401 acctgtgctt agataagatt aatgttcttc ataaaccacc atatgaacat ccaaaagact 5461 tgaagctcag tgatggtcgg ctgcgtgtag gatatgtgag ttccgacttt gggaatcatc 5521 ctacttctca ccttatgcag tctattccag gcatgcacaa tcctgataaa tttgaggtgt 5581 tctgttatgc cctgagccca gacgatggca caaacttccg agtgaaggtg atggcagaag 5641 ccaatcattt cattgatctt tctcagattc catgcaatgg aaaagcagct gatcgcatcc 5701 atcaggatgg aattcatatc cttgtaaata tgaatggcta tactaagggc gctcgaaatg 5761 agctttttgc tctcaggcca gctcctattc aggcaatgtg gctgggatac cctgggacga 5821 gtggtgcgct tttcatggat tatattatca ctgatcagga aacttcgcca gctgaagttg 5881 ctgagcagta ttccgagaaa ttggcttata tgccccacac tttttttatt ggtgatcatg 5941 ctaatatgtt ccctcacctg aagaaaaaag cagtcatcga ttttaagtcc aatgggcaca 6001 tttatgacaa tcggatagtt ctgaatggca tcgacctcaa agcatttctt gatagtctac 6061 cagatgtgaa aattgtcaag atgaagtgtc ctgatggagg agacaatgca gatagcagta 6121 acacagctct taatatgcct gttattccta tgaatactat tgcagaagca gttattgaaa 6181 tgattaaccg aggacagatt caaataacaa ttaatggatt cagtattagc aatggactgg 6241 caactactca gatcaacaat aaggctgcaa ctggagagga ggttccccgt accattattg 6301 taaccacccg ttctcagtac gggttaccag aagatgccat cgtatactgt aactttaatc 6361 agttgtataa aattgaccct tctactttgc agatgtgggc aaacattctg aagcgtgttc 6421 ccaatagtgt actctggctg ttgcgttttc cagcagtagg agaacctaat attcaacagt 6481 atgcacaaaa catgggcctg ccccagaacc gtatcatttt ttcacctgtt gctcctaaag 6541 aggaacacgt caggagaggc cagctggctg atgtctgctt ggacactcca ctctgtaatg 6601 ggcacaccac agggatggat gtcctctggg cagggacccc catggtgact atgccaggag 6661 agactcttgc ttctcgagtt gcagcatccc agctcacttg cttaggttgt cttgagctta 6721 ttgctaaaaa cagacaagaa tatgaagaca tagctgtgaa gctgggaact gatctagaat 6781 acctgaagaa agttcgtggc aaagtctgga agcaaagaat atctagccct ctgttcaaca 6841 ccaaacaata cacaatggaa ctagagcggc tctatctaca gatgtgggag cattatgcag 6901 ctggcaacaa acctgaccac atgattaagc ctgttgaagt cactgagtca gcaTAGGACC 6961 CAGCTTTCTT GTACAAAGTG GTTGATAGCT TGGATCCAAT CAACCTCTGG ATTACAAAAT 7021 TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC 7081 TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT 7141 GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG 7201 CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG 7261 TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC 7321 CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT 7381 GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT 7441 GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG 7501 CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGA GATCTGCCTC GACTGTGCCT 7561 TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT 7621 GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG 7681 TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC 7741 AATAGCAGGC ATGCTGGGGA CTCGAGTTAA GGGCGAATTG CCGATTAGGA TCTTCCTAGA 7801 GCATGGCTAC GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAAGG AACCCCTAGT 7861 GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG GGCGACCAAA 7921 GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG CGCGCAGCCT 7981 TAATTAAGAG CTCATGGCGC GCCTAGGCCT TGACGGCCTT CCGCCAATTC GCCCTATAGT 8041 GAGTCGTATT ACGTCGCGCT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC 8101 TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG 8161 CGAAGAGGCC CGCACCGAAA CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGG 8221 AGCGCCCTGT AGCGGCCACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 8281 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 8341 TTTAACAAAA TATTAACGCT TACAATTTAG //