LOCUS PIGM-pGEc1-DEST 5764 bp DNA circular 23-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(5242..5371) /note="3'-ITR" misc_feature complement(4940..5154) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 4392..4933 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_recomb 4297..4319 /label=attB2 /note="attB2 site" misc_feature 4329..4352 /note="8xHis Tag" misc_feature 4356..4379 /note="StrepTag" PCR_frame 3002..4297 /note="Reading Frame" /set="Set 1" source 3006..4274 /dnas_title="PIGM-pGEc1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:3194 IMAGE:3357207" /tissue_type="Eye, retinoblastoma" /clone_lib="NIH_MGC_16" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3006..4274 /dnas_title="PIGM" /gene="PIGM" /gene_synonym="MGC29896" /db_xref="GeneID:93183" /db_xref="HGNC:18858" CDS 3006..4274 /dnas_title="phosphatidylinositol glycan anchor biosynthesis, class M" /gene="PIGM" /gene_synonym="MGC29896" /codon_start=1 /product="phosphatidylinositol glycan anchor biosynthesis, class M" /protein_id="AAH01803.1" /db_xref="GI:45708486" /db_xref="GeneID:93183" /db_xref="HGNC:18858" /translation="MGSTKHWGEWLLNLKVAPAGVFGVAFLARVALVFYGVFQDRTLH VRYTDIDYQVFTDAARFVTEGRSPYLRATYRYTPLLGWLLTPNIYLSELFGKFLFISC DLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVLMVLYL IKKRLVACAAVFYGFAVHMKIYPVTYILPITLHLLPDRDNDKSLRQFRYTFQACLYEL LKRLCNRAVLLFVAVAGLTFFALSFGFYYEYGWEFLEHTYFYHLTRRDIRHNFSPYFY MLYLTAESKWSFSLGIAAFLPQLILLSAVSFAYYRDLVFCCFLHTSIFVTFNKVCTSQ YFLWYLCLLPLVMPLVRMPWKRAVVLLMLWFIGQAMWLAPAYVLEFQGKNTFLFIWLA GLFFLLINCSILIQIISHYKEEPLTERIKYD" misc_feature 3006..4274 /note="PIGM coding region" misc_feature 4275..4295 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4304) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4304) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4305..5764,1..2991) /source="pGEc1-DEST" /type="Destination Vector" clone 2992..4304 /source="PIGM-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggg ctccaccaag cactggggcg aatggctcct gaacttgaag gtggctccag 3061 ccggcgtctt tggtgtggcc tttctagcca gagtcgccct ggttttctat ggcgtcttcc 3121 aggaccggac cctgcacgtg aggtatacgg acatcgacta ccaggtcttc accgacgccg 3181 cgcgcttcgt cacggagggg cgctcgcctt acctgagagc cacgtaccgt tacaccccgc 3241 tgctgggttg gctcctcact cccaacatct acctcagcga gctctttgga aagtttctct 3301 tcatcagctg cgacctcctc accgctttcc tcttataccg cctgctgctg ctgaaggggc 3361 tggggcgccg ccaggcttgt ggctactgtg tcttttggct tcttaacccc ctgcctatgg 3421 cagtatccag ccgcggtaat gcggactcta ttgtcgcctc cctggtcctg atggtcctct 3481 acttgataaa gaaaagactc gtcgcgtgtg cagctgtatt ctatggtttc gcggtgcata 3541 tgaagatata tccagtgact tacatccttc ccataaccct ccacctgctt ccagatcgcg 3601 acaatgacaa aagcctccgt caattccggt acactttcca ggcttgtttg tacgagctcc 3661 tgaaaaggct gtgtaatcgg gctgtgctgc tgtttgtagc agttgctgga ctcacgtttt 3721 ttgccctgag ctttggtttt tactatgagt acggctggga atttttggaa cacacctact 3781 tttatcacct gactaggcgg gatatccgtc acaacttttc tccgtacttc tacatgctgt 3841 atttgactgc agagagcaag tggagttttt ccctgggaat tgctgcattc ctgccacagc 3901 tcatcttgct ttcagctgtg tctttcgcct attacagaga cctcgttttt tgttgttttc 3961 ttcatacgtc catttttgtg acttttaaca aagtctgcac ctcccagtac tttctttggt 4021 acctctgctt actgcctctt gtgatgccac tagtcagaat gccttggaaa agagctgtag 4081 ttctcctaat gttatggttt atagggcagg ccatgtggct ggctcctgcc tatgttctag 4141 agtttcaagg aaagaacacc tttctgttta tttggttagc tggtttgttc tttcttctta 4201 tcaattgttc catcctgatt caaattattt cccattacaa agaagaaccc ctgacagaga 4261 gaatcaaata tgacgaaaac ttgtactttc aaggctCCAG CTTTCTTGTA CAAAGTGGTG 4321 ATGGCCGCCA TCATCATCAT CATCATCATC ATGCGTGGAG CCACCCGCAG TTCGAAAAAT 4381 AATAAGGATC CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA 4441 ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA 4501 TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT 4561 ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG 4621 CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT 4681 TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG 4741 GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC 4801 CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC 4861 CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC 4921 TTCCGCGTCT TCGAGATCTG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG 4981 CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA 5041 AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT 5101 GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGACTCGAG 5161 TTAAGGGCGA ATTGCCGATT AGGATCTTCC TAGAGCATGG CTACGTAGAT AAGTAGCATG 5221 GCGGGTTAAT CATTAACTAC AAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC 5281 GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC 5341 GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCCTTAATTA AGAGCTCATG GCGCGCCTAG 5401 GCCTTGACGG CCTTCCGCCA ATTCGCCCTA TAGTGAGTCG TATTACGTCG CGCTCACTGG 5461 CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG 5521 CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GAAACGCCCT 5581 TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGGAGCGCC CTGTAGCGGC CACTCAACCC 5641 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA 5701 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT 5761 TTAG //