LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2222..2318) /label=attL2 /note="attL2 (c)" misc_signal complement(2333..2352) /note="T7 Promoter/priming site (c)" primer_bind 2360..2376 /note="M13 Reverse priming site" gene 2489..3298 /note="Kanamycin resistance gene" rep_origin 3419..4092 /note="pUC origin" vector join(2230..4095,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2215 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:60130 IMAGE:6196058" /tissue_type="Peripheral Nervous System, sympathetic trunk" /clone_lib="Lupski_sympathetic_trunk" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..2215 /gene="MGAT4B" /gene_synonym=GNT-IV /gene_synonym=GNT-IVB /db_xref="GeneID:11282" /db_xref="HGNC:7048" /db_xref="MIM:604561" CDS 692..2215 /dnas_title="mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B" /gene="MGAT4B" /gene_synonym=GNT-IV /gene_synonym=GNT-IVB /codon_start=1 /product="mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B" /protein_id="AAH51835.1" /db_xref="GI:30354276" /db_xref="GeneID:11282" /db_xref="HGNC:7048" /db_xref="MIM:604561" /translation="MRLRNGTFLTLLLFCLCAFLSLSWYAALSGQKGDVVDVYQREFL ALRDRLHAAEQESLKRSKELNLVLDEIKRAVSERQALRDGDGNRTWGRLTEDPRLKPW NGSHRHVLHLPTVFHHLPHLLAKESSLQPAVRVGQGRTGVSVVMGIPSVRREVHSYLT DTLHSLISELSPQEKEDSVIVVLIAETDSQYTSAVTENIKALFPTEIHSGLLEVISPS PHFYPDFSRLRESFGDPKERVRWRTKQNLDYCFLMMYAQSKGIYYVQLEDDIVAKPNY LSTMKNFALQQPSEDWMILEFSQLGFIGKMFKSLDLSLIVEFILMFYRDKPIDWLLDH ILWVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQAL RKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFWAFTPAAGDFIRFRFFQPLRLERFF FRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATFRYPRSPDGYLQIGSFYK GVAEGEVDPAFGPLEALRLSIQTDSPVWVILSEIFLKKAD" misc_difference 745..745 /gene="MGAT4B" /gene_synonym=GNT-IV /gene_synonym=GNT-IVB /note="'G' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 692..2215 /note="MGAT4B coding region" misc_difference 2042..2042 /gene="MGAT4B" /gene_synonym=GNT-IV /gene_synonym=GNT-IVB /note="'T' in cDNA is 'C' in the human genome; amino acid difference: 'F' in cDNA, 'L' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ccgggagttc ctggcgctgc gcgatcggtt 721 gcacgcagct gagcaggaga gcctgaagcg ctccaaggag ctcaacctgg tgctggacga 781 gatcaagagg gccgtgtcag aaaggcaggc gctgcgagac ggagacggca atcgcacctg 841 gggccgccta acagaggacc cccgattgaa gccgtggaac ggctcacacc ggcacgtgct 901 gcacctgccc accgtcttcc atcacctgcc acacctgctg gccaaggaga gcagtctgca 961 gcccgcggtg cgcgtgggcc agggccgcac cggagtgtcg gtggtgatgg gcatcccgag 1021 cgtgcggcgc gaggtgcact cgtacctgac tgacactctg cactcgctca tctccgagct 1081 gagcccgcag gagaaggagg actcggtcat cgtggtgctg atcgccgaga ctgactcaca 1141 gtacacttcg gcagtgacag agaacatcaa ggccttgttc cccacggaga tccattctgg 1201 gctcctggag gtcatctcac cctcccccca cttctaccct gacttctccc gcctccgaga 1261 gtcctttggg gaccccaagg agagagtcag gtggaggacc aaacagaacc tcgattactg 1321 cttcctcatg atgtacgcgc agtccaaagg catctactac gtgcagctgg aggatgacat 1381 cgtggccaag cccaactacc tgagcaccat gaagaacttt gcactgcagc agccttcaga 1441 ggactggatg atcctggagt tctcccagct gggcttcatt ggtaagatgt tcaagtcgct 1501 ggacctgagc ctgattgtag agttcattct catgttctac cgggacaagc ccatcgactg 1561 gctcctggac catattctgt gggtgaaagt ctgcaacccc gagaaggatg cgaagcactg 1621 tgaccggcag aaagccaacc tgcggatccg cttcaaaccg tccctcttcc agcacgtggg 1681 cactcactcc tcgctggctg gcaagatcca gaaactgaag gacaaagact ttggaaagca 1741 ggcgctgcgg aaggagcatg tgaacccgcc agcagaggtg agcacgagcc tgaagacata 1801 ccagcacttc accctggaga aagcctacct gcgcgaggac ttcttctggg ccttcacccc 1861 tgccgcgggg gacttcatcc gcttccgctt cttccaacct ctaagactgg agcggttctt 1921 cttccgcagt gggaacatcg agcacccgga ggacaagctc ttcaacacgt ctgtggaggt 1981 gctgcccttc gacaaccctc agtcagacaa ggaggccctg caggagggcc gcaccgccac 2041 cttccggtac cctcggagcc ccgacggcta cctccagatc ggctccttct acaagggagt 2101 ggcagaggga gaggtggacc cagccttcgg ccctctggaa gcactgcgcc tctcgatcca 2161 gacggactcc cctgtgtggg tgattctgag cgagatcttc ctgaaaaagg ccgacTAGGA 2221 CCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA 2281 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA 2341 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA 2401 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC 2461 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC 2521 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT 2581 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT 2641 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA 2701 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA 2761 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA 2821 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC 2881 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA 2941 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG 3001 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT 3061 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG 3121 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG 3181 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT 3241 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC 3301 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC 3361 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC 3421 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 3481 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 3541 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 3601 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 3661 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 3721 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 3781 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 3841 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 3901 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 3961 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 4021 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 4081 TTTTGCTCAC ATGTT //