LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1976..2072) /label=attL2 /note="attL2 (c)" misc_signal complement(2087..2106) /note="T7 Promoter/priming site (c)" primer_bind 2114..2130 /note="M13 Reverse priming site" gene 2243..3052 /note="Kanamycin resistance gene" rep_origin 3173..3846 /note="pUC origin" vector join(1984..3849,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1969 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:75408 IMAGE:30378187" /tissue_type="Testis" /clone_lib="NIH_MGC_180" /lab_host="DH10B" /note="Vector: pCMV-SPORT6.1" gene 692..1969 /gene="MGAT4C" /gene_synonym=GNTIVH /gene_synonym=HGNT-IV-H /db_xref="GeneID:25834" /db_xref="HGNC:30871" /db_xref="MIM:607385" CDS 692..1969 /dnas_title="mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme C (putative)" /gene="MGAT4C" /gene_synonym=GNTIVH /gene_synonym=HGNT-IV-H /codon_start=1 /product="mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme C (putative)" /protein_id="AAH64141.1" /db_xref="GI:39850232" /db_xref="GeneID:25834" /db_xref="HGNC:30871" /db_xref="MIM:607385" /translation="MFKFHQMKHIFEILDKMRCLRKRSTVSFLGVLVIFLLFMNLYIE DSYVLEGDKQLIRETSTHQLNSERYVHTFKDLSNFSGAINVTYRYLAATPLQRKRYLT IGLSSVKRKKGNYLLETIKSIFEQSSYEELKEISVVVHLADFNSSWRDAMVQDITQKF AHHIIAGRLMVIHAPEEYYPILDGLKRNYNDPEDRVKFRSKQNVDYAFLLNFCANTSD YYVMLEDDVRCSKNFLTAIKKVIASLEGTYWVTLEFSKLGYIGKLYHSHDLPRLAHFL LMFYQEMPCDWLLTHFRGLLAQKNVIRFKPSLFQHMGYYSSYKGTENKLKDDDFEEES FDIPDNPPASLYTNMNVFENYEASKAYSSVDEYFWGKPPSTGDVFVIVFENPIIIKKI KVNTGTEDRQNDILHHGALDVGENVMPSKQRRQCSSYLRLGEFKNGNFEMSGVNQKIP FDIHCMRIYVTKTQKEWLIIRSISIWTS" misc_feature 692..1969 /note="MGAT4C coding region" misc_difference 1818..1818 /gene="MGAT4C" /gene_synonym=GNTIVH /gene_synonym=HGNT-IV-H /note="'G' in cDNA is 'C' in the human genome; amino acid difference: 'S' in cDNA, 'T' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg caaacaactt ataagggaaa catccacaca 721 tcaactgaat tcagaacgct atgttcatac tttcaaggat ttatctaatt tctcaggagc 781 cataaatgtc acctatcgct acctagctgc cacaccttta caaagaaagc ggtatcttac 841 aattggactt tcttcagtaa agcgaaaaaa aggaaactat ttacttgaga caattaagtc 901 aatttttgag caatccagct atgaagagct gaaggaaatt tcagtggtgg ttcacctagc 961 agactttaat tcttcctggc gtgatgccat ggtccaggat attacacaga aatttgcgca 1021 ccatattatt gcaggaagat taatggttat acatgctcca gaggagtatt acccaatcct 1081 agatggcctt aaaagaaatt acaatgatcc agaagataga gtcaaatttc gttccaagca 1141 aaatgtagat tatgcttttc tgcttaattt ttgtgccaat acttcagact attatgtaat 1201 gcttgaagat gatgttcgat gttcaaaaaa tttcttaact gccatcaaga aagtcattgc 1261 atccctagaa ggaacttact gggtaactct tgaattctct aagcttggct acattggtaa 1321 actctatcat tctcatgatc tcccacgttt ggcccatttt ttattaatgt tttatcaaga 1381 aatgccttgt gattggctat tgactcattt ccgtggtctg ttggctcaga aaaatgtgat 1441 ccgttttaaa ccatctctct ttcagcacat gggctattat tcatcataca aagggacgga 1501 gaataagctg aaggatgatg attttgaaga ggagtcattt gacattcctg ataacccccc 1561 tgcaagtctg tacaccaaca tgaatgtgtt tgaaaattat gaagcaagca aggcttacag 1621 tagtgttgat gagtactttt gggggaaacc accttcaaca ggagatgttt ttgtgattgt 1681 atttgaaaat ccaattataa taaaaaaaat taaagtaaat actggaacag aagatcggca 1741 aaatgatatt ttgcatcatg gagccctaga tgttggggaa aacgttatgc ctagcaaaca 1801 aaggagacaa tgttctagtt acttaagact aggagaattc aaaaatggaa actttgaaat 1861 gtcaggtgta aatcaaaaaa ttccatttga tatacattgt atgaggatat atgtcaccaa 1921 aacacaaaag gaatggctaa ttattaggag tattagcatt tggacttctT AGGACCCAGC 1981 TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA 2041 GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGATATCC CCTATAGTGA 2101 GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG 2161 ATGTTACATT GCACAAGATA AAATAATATC ATCATGAACA ATAAAACTGT CTGCTTACAT 2221 AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCGA GGCCGCGATT 2281 AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA 2341 ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA 2401 ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT 2461 GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG 2521 GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA 2581 TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC 2641 TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG 2701 AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT 2761 TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC 2821 TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT 2881 TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG 2941 CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA 3001 TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT 3061 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC 3121 ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 3181 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 3241 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 3301 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC 3361 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 3421 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 3481 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 3541 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG 3601 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 3661 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 3721 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 3781 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 3841 TCACATGTT //