LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2216..2312) /label=attL2 /note="attL2 (c)" misc_signal complement(2327..2346) /note="T7 Promoter/priming site (c)" primer_bind 2354..2370 /note="M13 Reverse priming site" gene 2483..3292 /note="Kanamycin resistance gene" rep_origin 3413..4086 /note="pUC origin" PCR_frame 669..2216 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2236) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2233) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2224..4089,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2194..2214 /note="TEV site" source 673..2193 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:4979 IMAGE:3452687" /tissue_type="Placenta, choriocarcinoma" /clone_lib="NIH_MGC_10" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..2193 /gene="ALG6" /db_xref="GeneID:29929" /db_xref="HGNC:23157" /db_xref="MIM:604566" CDS 673..2193 /dnas_title="asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S. cerevisiae)" /gene="ALG6" /codon_start=1 /product="asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S. cerevisiae)" /protein_id="AAH01253.1" /db_xref="GI:12654821" /db_xref="GeneID:29929" /db_xref="HGNC:23157" /db_xref="MIM:604566" /translation="MEKWYLMTVVVLIGLTVRWTVSLNSYSGAGKPPMFGDYEAQRHW QEITFNLPVKQWYFNSSDNNLQYWGLDYPPLTAYHSLLCAYVAKFINPDWIALHTSRG YESQAHKLFMRTTVLIADLLIYIPAVVLYCCCLKEISTKKKIANALCILLYPGLILID YGHFQYNSVSLGFALWGVLGISCDCDLLGSLAFCLAINYKQMELYHALPFFCFLLGKC FKKGLKGKGFVLLVKLACIVVASFVLCWLPFFTEREQTLQVLRRLFPVDRGLFEDKVA NIWCSFNVFLKIKDILPRHIQLIMSFCFTFLSLLPACIKLILQPSSKGFKFTLVSCAL SFFLFSFQVHEKSILLVSLPVCLVLSEIPFMSTWFLLVSTFSMLPLLLKDELLMPSVV TTMAFFIACVTSFSIFEKTSEEELQLKSFSISVRKYLPCFTFLSRIIQYLFLISVITM VLLTLMTVTLDPPQKLPDLFSVLVCFVSCLNFLFFLVYFNIIIMWDSKSGRNQKKIS" misc_feature 673..2193 /note="ALG6 coding region" misc_difference 1583..1583 /gene="ALG6" /note="'T' in cDNA is 'C' in the human genome; amino acid difference: 'F' in cDNA, 'S' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggagaa atggtacttg atgacagtag tggttttaat aggactaaca 721 gtacgatgga cagtgtctct taattcttat tcaggtgctg gtaaaccgcc tatgtttggt 781 gattatgaag ctcagagaca ctggcaagaa ataactttta atttaccggt caaacaatgg 841 tattttaaca gcagtgataa caatttacag tattggggat tggattaccc acctcttaca 901 gcttatcata gtctcctatg tgcatatgtg gcaaagttta taaatccaga ctggattgct 961 ctccatacat cacgtggata tgagagtcag gcacataagc tcttcatgcg tacaacagtt 1021 ttaattgctg atctgctgat ttacatacct gcagtggttt tgtactgttg ttgcttaaaa 1081 gaaatctcaa ctaagaaaaa gattgctaat gcattatgca tcttgctgta tccaggcctt 1141 attcttatag actatggaca ttttcaatat aattctgtga gtcttggctt tgctttgtgg 1201 ggtgttcttg gaatatcttg tgactgcgac ctcctagggt cactggcatt ttgcttagct 1261 ataaattata aacagatgga actttaccac gccttgccat ttttttgctt tttacttggc 1321 aagtgtttta aaaaaggcct caaaggaaag gggtttgtgt tgctagttaa gctagcttgt 1381 attgttgtgg cttccttcgt tctctgctgg ctgccattct ttacagaaag ggaacaaacc 1441 ctgcaggttc taagaagact cttcccggtt gatcgtggat tatttgagga taaagtagcc 1501 aatatttggt gcagcttcaa tgtctttctg aagattaagg atattttgcc acgtcacatc 1561 caattaataa tgagcttttg ttttacgttt ttgagcctgc ttcctgcatg cataaaatta 1621 atacttcagc cctcttccaa aggattcaaa tttacactgg ttagctgtgc gctatcattc 1681 tttttatttt ctttccaagt acatgaaaaa tccattctct tggtgtcact accagtctgc 1741 ttagttttaa gtgaaattcc ttttatgtct acttggtttt tacttgtgtc aacatttagt 1801 atgctacctc ttctattgaa ggatgaactc ctaatgccct ctgttgtgac aacaatggca 1861 ttttttatag cttgtgtaac ttccttttca atatttgaaa agacttctga agaagaactg 1921 cagttgaaat ccttttccat ttctgtgagg aaatatcttc catgttttac atttctttcc 1981 agaattatac aatatttgtt tcttatctca gtcatcacta tggtgcttct gacgttgatg 2041 actgtcacac tggatcctcc tcagaaacta ccggacttgt tttctgtatt ggtgtgtttt 2101 gtatcttgct tgaacttcct gttcttcttg gtatacttta acattattat tatgtgggat 2161 tccaaaagtg gaagaaatca gaagaaaatc agcgaaaact tgtactttca aggctCCAGC 2221 TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA 2281 GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGATATCC CCTATAGTGA 2341 GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG 2401 ATGTTACATT GCACAAGATA AAATAATATC ATCATGAACA ATAAAACTGT CTGCTTACAT 2461 AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCGA GGCCGCGATT 2521 AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA 2581 ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA 2641 ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT 2701 GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG 2761 GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA 2821 TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC 2881 TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG 2941 AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT 3001 TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC 3061 TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT 3121 TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG 3181 CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA 3241 TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT 3301 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC 3361 ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 3421 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 3481 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 3541 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC 3601 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 3661 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 3721 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 3781 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG 3841 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 3901 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 3961 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 4021 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 4081 TCACATGTT //