LOCUS ALG6-pGEc2-DEST 6742 bp DNA circular 23-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6220..6349) /note="3'-ITR" misc_feature complement(5918..6132) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5370..5911 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4549..4571 /note="attB2" misc_feature 5349..5354 /note="BstB1 site" misc_feature 4572..5267 /note="Super GFP" misc_feature 5268..5318 /note="AviTag" misc_feature 5319..5342 /note="His Tag" PCR_frame 3002..4549 /note="Reading Frame" /set="Set 1" source 3006..4526 /dnas_title="ALG6-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:4979 IMAGE:3452687" /tissue_type="Placenta, choriocarcinoma" /clone_lib="NIH_MGC_10" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..4526 /dnas_title="ALG6" /gene="ALG6" /db_xref="GeneID:29929" /db_xref="HGNC:23157" /db_xref="MIM:604566" CDS 3006..4526 /dnas_title="asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S. cerevisiae)" /gene="ALG6" /codon_start=1 /product="asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S. cerevisiae)" /protein_id="AAH01253.1" /db_xref="GI:12654821" /db_xref="GeneID:29929" /db_xref="HGNC:23157" /db_xref="MIM:604566" /translation="MEKWYLMTVVVLIGLTVRWTVSLNSYSGAGKPPMFGDYEAQRHW QEITFNLPVKQWYFNSSDNNLQYWGLDYPPLTAYHSLLCAYVAKFINPDWIALHTSRG YESQAHKLFMRTTVLIADLLIYIPAVVLYCCCLKEISTKKKIANALCILLYPGLILID YGHFQYNSVSLGFALWGVLGISCDCDLLGSLAFCLAINYKQMELYHALPFFCFLLGKC FKKGLKGKGFVLLVKLACIVVASFVLCWLPFFTEREQTLQVLRRLFPVDRGLFEDKVA NIWCSFNVFLKIKDILPRHIQLIMSFCFTFLSLLPACIKLILQPSSKGFKFTLVSCAL SFFLFSFQVHEKSILLVSLPVCLVLSEIPFMSTWFLLVSTFSMLPLLLKDELLMPSVV TTMAFFIACVTSFSIFEKTSEEELQLKSFSISVRKYLPCFTFLSRIIQYLFLISVITM VLLTLMTVTLDPPQKLPDLFSVLVCFVSCLNFLFFLVYFNIIIMWDSKSGRNQKKIS" misc_feature 3006..4526 /note="ALG6 coding region" misc_difference 3916..3916 /dnas_title="ALG6" /gene="ALG6" /note="'T' in cDNA is 'C' in the human genome; amino acid difference: 'F' in cDNA, 'S' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_feature 4527..4547 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4556) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4556) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4557..6742,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4556 /source="ALG6-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgga gaaatggtac ttgatgacag tagtggtttt aataggacta acagtacgat 3061 ggacagtgtc tcttaattct tattcaggtg ctggtaaacc gcctatgttt ggtgattatg 3121 aagctcagag acactggcaa gaaataactt ttaatttacc ggtcaaacaa tggtatttta 3181 acagcagtga taacaattta cagtattggg gattggatta cccacctctt acagcttatc 3241 atagtctcct atgtgcatat gtggcaaagt ttataaatcc agactggatt gctctccata 3301 catcacgtgg atatgagagt caggcacata agctcttcat gcgtacaaca gttttaattg 3361 ctgatctgct gatttacata cctgcagtgg ttttgtactg ttgttgctta aaagaaatct 3421 caactaagaa aaagattgct aatgcattat gcatcttgct gtatccaggc cttattctta 3481 tagactatgg acattttcaa tataattctg tgagtcttgg ctttgctttg tggggtgttc 3541 ttggaatatc ttgtgactgc gacctcctag ggtcactggc attttgctta gctataaatt 3601 ataaacagat ggaactttac cacgccttgc catttttttg ctttttactt ggcaagtgtt 3661 ttaaaaaagg cctcaaagga aaggggtttg tgttgctagt taagctagct tgtattgttg 3721 tggcttcctt cgttctctgc tggctgccat tctttacaga aagggaacaa accctgcagg 3781 ttctaagaag actcttcccg gttgatcgtg gattatttga ggataaagta gccaatattt 3841 ggtgcagctt caatgtcttt ctgaagatta aggatatttt gccacgtcac atccaattaa 3901 taatgagctt ttgttttacg tttttgagcc tgcttcctgc atgcataaaa ttaatacttc 3961 agccctcttc caaaggattc aaatttacac tggttagctg tgcgctatca ttctttttat 4021 tttctttcca agtacatgaa aaatccattc tcttggtgtc actaccagtc tgcttagttt 4081 taagtgaaat tccttttatg tctacttggt ttttacttgt gtcaacattt agtatgctac 4141 ctcttctatt gaaggatgaa ctcctaatgc cctctgttgt gacaacaatg gcatttttta 4201 tagcttgtgt aacttccttt tcaatatttg aaaagacttc tgaagaagaa ctgcagttga 4261 aatccttttc catttctgtg aggaaatatc ttccatgttt tacatttctt tccagaatta 4321 tacaatattt gtttcttatc tcagtcatca ctatggtgct tctgacgttg atgactgtca 4381 cactggatcc tcctcagaaa ctaccggact tgttttctgt attggtgtgt tttgtatctt 4441 gcttgaactt cctgttcttc ttggtatact ttaacattat tattatgtgg gattccaaaa 4501 gtggaagaaa tcagaagaaa atcagcgaaa acttgtactt tcaaggctCC AGCTTTCTTG 4561 TACAAAGTGG TATGAGCAAG GGCGAGGAAC TGTTCACCGG CGTGGTGCCC ATCCTGGTGG 4621 AACTGGATGG CGACGTGAAC GGCCACAAGT TCTCTGTGCG GGGAGAGGGC GAAGGCGACG 4681 CCACAAATGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCTT 4741 GGCCTACCCT CGTGACCACA CTGACCTACG GCGTGCAGTG CTTCAGCAGA TACCCCGACC 4801 ATATGAAGCG GCACGACTTC TTCAAGAGCG CCATGCCCGA GGGCTACGTG CAGGAACGGA 4861 CCATCAGCTT CAAGGACGAC GGCACCTACA AGACCAGAGC CGAAGTGAAG TTCGAGGGCG 4921 ACACCCTCGT GAACCGGATC GAGCTGAAGG GCATCGATTT CAAAGAGGAC GGCAACATCC 4981 TGGGCCACAA GCTGGAGTAC AACTTCAACA GCCACAACGT GTACATCACC GCCGACAAGC 5041 AGAAGAACGG CATCAAGGCC AACTTCAAGA TCCGGCACAA CGTGGAAGAT GGCAGCGTGC 5101 AGCTGGCCGA CCACTACCAG CAGAACACCC CCATCGGAGA TGGCCCCGTG CTGCTGCCCG 5161 ACAACCACTA CCTGAGCACC CAGAGCGTGC TGAGCAAGGA CCCCAACGAG AAGCGGGACC 5221 ACATGGTGCT GCTGGAATTC GTGACCGCCG CTGGCATCAC CCACGGCATG AGCGGCCTGA 5281 ACGACATCTT CGAGGCCCAG AAAATCGAGT GGCACGAGCA CCACCATCAT CACCATCACC 5341 ACTGATGATT CGAAAAATAA TAAGGATCCA ATCAACCTCT GGATTACAAA ATTTGTGAAA 5401 GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA 5461 TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT 5521 CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT 5581 GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC 5641 TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC 5701 TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG 5761 GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA 5821 CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC 5881 TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GAGATCTGCC TCGACTGTGC CTTCTAGTTG 5941 CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC 6001 CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC 6061 TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG 6121 GCATGCTGGG GACTCGAGTT AAGGGCGAAT TGCCGATTAG GATCTTCCTA GAGCATGGCT 6181 ACGTAGATAA GTAGCATGGC GGGTTAATCA TTAACTACAA GGAACCCCTA GTGATGGAGT 6241 TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA AAGGTCGCCC 6301 GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC CTTAATTAAG 6361 AGCTCATGGC GCGCCTAGGC CTTGACGGCC TTCCGCCAAT TCGCCCTATA GTGAGTCGTA 6421 TTACGTCGCG CTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA 6481 CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG 6541 CCCGCACCGA AACGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGAGCGCCCT 6601 GTAGCGGCCA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT 6661 TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA 6721 AATATTAACG CTTACAATTT AG //