LOCUS ALG8-pGEc2-DEST 6799 bp DNA circular 23-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6277..6406) /note="3'-ITR" misc_feature complement(5975..6189) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5427..5968 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4606..4628 /note="attB2" misc_feature 5406..5411 /note="BstB1 site" misc_feature 4629..5324 /note="Super GFP" misc_feature 5325..5375 /note="AviTag" misc_feature 5376..5399 /note="His Tag" PCR_frame 3002..4606 /note="Reading Frame" /set="Set 1" source 3006..4583 /dnas_title="ALG8-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:2840 IMAGE:2966827" /tissue_type="Skin, melanotic melanoma." /clone_lib="NIH_MGC_20" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3006..4583 /dnas_title="ALG8" /gene="ALG8" /db_xref="GeneID:79053" /db_xref="HGNC:23161" /db_xref="MIM:608103" CDS 3006..4583 /dnas_title="asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae)" /gene="ALG8" /codon_start=1 /product="asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae)" /protein_id="AAH01133.1" /db_xref="GI:12654595" /db_xref="GeneID:79053" /db_xref="HGNC:23161" /db_xref="MIM:608103" /translation="MAALTIATGTGNWFSALALGVTLLKCLLIPTYHSTDFEVHRNWL AITHSLPISQWYYEATSEWTLDYPPFFAWFEYILSHVAKYFDQEMLNVHNLNYSSSRT LLFQRFSVIFMDVLFVYAVRECCKCIDGKKVGKELTEKPKFILSVLLLWNFGLLIVDH IHFQYNGFLFGLMLLSIARLFQKRHMEGAFLFAVLLHFKHIYLYVAPAYGVYLLRSYC FTANKPDGSIRWKSFSFVRVISLGLVVFLVSALSLGPFLALNQLPQVFSRLFPFKRGL CHAYWAPNFWALYNALDKVLSVIGLKLKFLDPNNIPKASMTSGLVQQFQHTVLPSVTP LATLICTLIAILPSIFCLWFKPQGPRGFLRCLTLCALSSFMFGWHVHEKAILLAILPM SLLSVGKAGDASIFLILTTTGHYSLFPLLFTAPELPIKILLMLLFTIYSISSLKTLFR KEKPLFNWMETFYLLGLGPLEVCCEFVFPFTSWKVKYPFIPLLLTSVYCAVGVTYAWF KLYVSVLIDSAIGKTKKQ" misc_feature 3006..4583 /note="ALG8 coding region" misc_feature 4584..4604 /note="TEV site" misc_difference 4512..4512 /dnas_title="ALG8" /gene="ALG8" /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'I' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4613) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4613) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4614..6799,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4613 /source="ALG8-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggc ggcgctcaca attgccacgg gtactggcaa ttggttttcg gctttggcgc 3061 tcggggtgac tcttctcaaa tgccttctca tccccacata ccattccaca gattttgaag 3121 tacaccgaaa ctggcttgct atcactcaca gtttgccaat atcacagtgg tattatgagg 3181 caacttcaga gtggacgttg gattaccccc ctttctttgc atggtttgag tatatcctgt 3241 cacatgttgc caaatatttt gatcaagaaa tgctgaatgt ccataatttg aattactcca 3301 gctcaaggac cttacttttc cagagatttt ccgtcatctt tatggatgta ctctttgtgt 3361 atgctgtccg tgagtgctgt aaatgcattg atggaaaaaa agtgggtaaa gaacttacag 3421 aaaagccaaa atttattctg tcggtattac ttctgtggaa cttcgggtta ttaattgtgg 3481 accatattca ttttcagtac aatggctttt tatttggatt aatgctactc tccattgcac 3541 gattatttca gaaaaggcat atggaaggag catttctctt tgctgttctc ctacatttca 3601 agcatatcta cctctatgta gcaccagctt atggtgtata tctgctgcga tcctactgtt 3661 tcactgcaaa taaaccagat gggtctattc gatggaagag tttcagcttt gttcgtgtta 3721 tttccctggg actggttgtt ttcttagttt ctgctctttc attgggtcct ttcctggcct 3781 tgaatcagct gcctcaagtc ttttcccgac tctttccttt caagaggggc ctctgtcatg 3841 catattgggc tccaaacttc tgggctttgt acaatgcttt ggacaaagtg ctgtctgtca 3901 tcggtttgaa attgaaattt cttgatccca acaatattcc caaggcctca atgacaagtg 3961 gtttggttca gcagttccaa cacacagtcc ttccctcagt gactcccttg gcaaccctca 4021 tctgcacact gattgccata ttgccctcta ttttctgtct ttggtttaaa ccccaagggc 4081 ccagaggctt tctccgatgt ctaactcttt gtgccttgag ctcctttatg tttgggtggc 4141 atgttcatga aaaagccata cttctagcaa ttctcccaat gagccttttg tctgtgggaa 4201 aagcaggaga cgcttcgatt tttctgattc tgaccacaac aggacattat tccctctttc 4261 ctctgctctt cactgcacca gaacttccca ttaaaatctt actcatgtta ctattcacca 4321 tatatagtat ttcgtcactg aagactttat tcagaaaaga aaaacctctt tttaattgga 4381 tggaaacttt ctacctgctt ggcctggggc ctctggaagt ctgctgtgaa tttgtattcc 4441 ctttcacctc ctggaaggtg aagtacccct tcatcccttt gttactaacc tcagtgtatt 4501 gtgcagtagg cgtcacatat gcttggttca aactgtatgt ttcagtattg attgactctg 4561 ctattggcaa gacaaagaaa caagaaaact tgtactttca aggctCCAGC TTTCTTGTAC 4621 AAAGTGGTAT GAGCAAGGGC GAGGAACTGT TCACCGGCGT GGTGCCCATC CTGGTGGAAC 4681 TGGATGGCGA CGTGAACGGC CACAAGTTCT CTGTGCGGGG AGAGGGCGAA GGCGACGCCA 4741 CAAATGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCTTGGC 4801 CTACCCTCGT GACCACACTG ACCTACGGCG TGCAGTGCTT CAGCAGATAC CCCGACCATA 4861 TGAAGCGGCA CGACTTCTTC AAGAGCGCCA TGCCCGAGGG CTACGTGCAG GAACGGACCA 4921 TCAGCTTCAA GGACGACGGC ACCTACAAGA CCAGAGCCGA AGTGAAGTTC GAGGGCGACA 4981 CCCTCGTGAA CCGGATCGAG CTGAAGGGCA TCGATTTCAA AGAGGACGGC AACATCCTGG 5041 GCCACAAGCT GGAGTACAAC TTCAACAGCC ACAACGTGTA CATCACCGCC GACAAGCAGA 5101 AGAACGGCAT CAAGGCCAAC TTCAAGATCC GGCACAACGT GGAAGATGGC AGCGTGCAGC 5161 TGGCCGACCA CTACCAGCAG AACACCCCCA TCGGAGATGG CCCCGTGCTG CTGCCCGACA 5221 ACCACTACCT GAGCACCCAG AGCGTGCTGA GCAAGGACCC CAACGAGAAG CGGGACCACA 5281 TGGTGCTGCT GGAATTCGTG ACCGCCGCTG GCATCACCCA CGGCATGAGC GGCCTGAACG 5341 ACATCTTCGA GGCCCAGAAA ATCGAGTGGC ACGAGCACCA CCATCATCAC CATCACCACT 5401 GATGATTCGA AAAATAATAA GGATCCAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT 5461 TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC 5521 CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT 5581 GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA 5641 CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT 5701 CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG 5761 CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA 5821 AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT 5881 CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC 5941 CGGCTCTGCG GCCTCTTCCG CGTCTTCGAG ATCTGCCTCG ACTGTGCCTT CTAGTTGCCA 6001 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC 6061 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT 6121 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA 6181 TGCTGGGGAC TCGAGTTAAG GGCGAATTGC CGATTAGGAT CTTCCTAGAG CATGGCTACG 6241 TAGATAAGTA GCATGGCGGG TTAATCATTA ACTACAAGGA ACCCCTAGTG ATGGAGTTGG 6301 CCACTCCCTC TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC 6361 GCCCGGGCTT TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCCTT AATTAAGAGC 6421 TCATGGCGCG CCTAGGCCTT GACGGCCTTC CGCCAATTCG CCCTATAGTG AGTCGTATTA 6481 CGTCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC 6541 AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC 6601 GCACCGAAAC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGGA GCGCCCTGTA 6661 GCGGCCACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC 6721 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT 6781 ATTAACGCTT ACAATTTAG //