LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2114..2210) /label=attL2 /note="attL2 (c)" misc_signal complement(2225..2244) /note="T7 Promoter/priming site (c)" primer_bind 2252..2268 /note="M13 Reverse priming site" gene 2381..3190 /note="Kanamycin resistance gene" rep_origin 3311..3984 /note="pUC origin" PCR_frame 669..2114 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2134) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2131) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2122..3987,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2092..2112 /note="TEV site" source 673..2091 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:88353 IMAGE:3839674" /tissue_type="Brain, glioblastoma" /clone_lib="NIH_MGC_57" /lab_host="DH10B" /note="Vector: pDNR-LIB" gene 673..2091 /gene="ALG10" /gene_synonym=DIE2 /gene_synonym=KCR1 /db_xref="GeneID:84920" /db_xref="HGNC:23162" /db_xref="MIM:603313" CDS 673..2091 /dnas_title="ALG10 protein" /gene="ALG10" /gene_synonym=DIE2 /gene_synonym=KCR1 /codon_start=1 /product="ALG10 protein" /protein_id="AAH70347.1" /db_xref="GI:47125451" /db_xref="GeneID:84920" /db_xref="HGNC:23162" /db_xref="MIM:603313" /translation="MAQLEGYYFSAALSCTFLVSCLLFSAFSRALREPYMDEIFHLPQ AQRYCEGHFSLSQWDPMITTLPGLYLVSIGVIKPAIWIFGWSEHVVCSIGMLRFVNLL FSVGNFYLLYLLFCKVQPRNKAASSIQRVLSTLTLAVFPTLYFFNFLYYTEAGSMFFT LFAYLMCLYGNHKTSAFLGFCGFVFRQTNIIWAVFCAGNVIAQKLTEAWKTELQKKED RLPPIKGPFAEFRKILQFLLAYSMSFKNLSMLLLLTWPYILLGFLFCAFVVVNGGIVI GDRSSHEACLHFPQLFYFFSFTLFFSFPHLLSPSKIKTFLSLVWKRRILFFVVTLVSV FLVWKFTYAHKYLLADNRHYTFYVWKRVFQRYETVKYLLVPAYIFAGWSIADSLKSKS IFWNLMFFICLFTVIVPQKLLEFRYFILPYVIYRLNIPLPPTSRLICELSCYAVVNFI TFFIFLNKTFQWPNSQDIQRFMW" misc_feature 673..2091 /note="ALG10 coding region" misc_difference 1222..1222 /gene="ALG10" /gene_synonym=DIE2 /gene_synonym=KCR1 /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'M' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggcgca gctggaaggt tactatttct cggccgcctt gagctgtacc 721 tttttagtat cctgcctcct cttctccgcc ttcagccggg cgttgcgaga gccctacatg 781 gacgagatct tccacctgcc tcaggcgcag cgctactgtg agggccattt ctccctttcc 841 cagtgggatc ccatgattac tacattacct ggcttgtacc tggtgtcaat tggagtgatc 901 aaacctgcca tttggatctt tggatggtct gaacatgttg tctgctccat tgggatgctc 961 agatttgtta atcttctctt cagtgttggc aacttctatt tactatattt gcttttctgc 1021 aaggtacaac ccagaaacaa ggctgcctca agtatccaga gagtcttgtc aacattaaca 1081 ctagcagtat ttccaacact ttattttttt aacttccttt attatacaga agcaggatct 1141 atgtttttta ctctttttgc gtatttgatg tgtctttatg gaaatcataa aacttcagcc 1201 ttccttggat tttgtggctt cgtgtttcgg caaacaaata tcatctgggc tgtcttctgt 1261 gcaggaaatg tcattgcaca aaagttaacg gaggcttgga aaactgagct acaaaagaag 1321 gaagacagac ttccacctat taaaggacca tttgcagaat tcagaaaaat tcttcagttt 1381 cttttggctt attccatgtc ctttaaaaac ttgagtatgc ttttgcttct gacttggccc 1441 tacatccttc tgggatttct gttttgtgct tttgtagtag ttaatggtgg aattgttatt 1501 ggcgatcgga gtagtcatga agcctgtctt cattttcctc aactattcta ctttttttca 1561 tttactctct ttttttcctt tcctcatctc ctgtctccta gcaaaattaa gacttttctt 1621 tccttagttt ggaaacgtag aattctgttt tttgtggtta ccttagtctc tgtgttttta 1681 gtttggaaat tcacttatgc tcataaatac ttgctagcag acaatagaca ttatactttc 1741 tatgtgtgga aaagagtttt tcaaagatat gaaactgtaa aatatttgtt agttccagcc 1801 tatatatttg ctggttggag tatagctgac tcattgaaat caaagtcaat tttttggaat 1861 ttaatgtttt tcatatgctt gttcactgtt atagttcctc agaaactgct ggaatttcgt 1921 tacttcattt taccttatgt catttatagg cttaacatac ctctgcctcc cacatccaga 1981 ctcatttgtg aactgagctg ctatgcagtt gttaatttca taactttttt catctttctg 2041 aacaagactt ttcagtggcc aaatagtcag gacattcaaa ggtttatgtg ggaaaacttg 2101 tactttcaag gctCCAGCTT TCTTGTACAA AGTTGGCATT ATAAGAAAGC ATTGCTTATC 2161 AATTTGTTGC AACGAACAGG TCACTATCAG TCAAAATAAA ATCATTATTT GCCATCCAGC 2221 TGATATCCCC TATAGTGAGT CGTATTACAT GGTCATAGCT GTTTCCTGGC AGCTCTGGCC 2281 CGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA ATAATATCAT CATGAACAAT 2341 AAAACTGTCT GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA 2401 AACGTCGAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC 2461 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGC TTGTATGGGA AGCCCGATGC 2521 GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT 2581 GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG 2641 TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC GGAAAAACAG CATTCCAGGT 2701 ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG 2761 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT 2821 CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA 2881 GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC 2941 ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG 3001 GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT 3061 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA 3121 AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA 3181 GTTTTTCTAA TCAGAATTGG TTAATTGGTT GTAACACTGG CAGAGCATTA CGCTGACTTG 3241 ACGGGACGGC GCAAGCTCAT GACCAAAATC CCTTAACGTG AGTTACGCGT CGTTCCACTG 3301 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT 3361 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA 3421 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC 3481 TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC 3541 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT 3601 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG 3661 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA 3721 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT 3781 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA 3841 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC 3901 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC 3961 CTTTTGCTGG CCTTTTGCTC ACATGTT //