LOCUS ALG10-pGEc2-DEST 6640 bp DNA circular 23-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6118..6247) /note="3'-ITR" misc_feature complement(5816..6030) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5268..5809 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4447..4469 /note="attB2" misc_feature 5247..5252 /note="BstB1 site" misc_feature 4470..5165 /note="Super GFP" misc_feature 5166..5216 /note="AviTag" misc_feature 5217..5240 /note="His Tag" PCR_frame 3002..4447 /note="Reading Frame" /set="Set 1" source 3006..4424 /dnas_title="ALG10-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:88353 IMAGE:3839674" /tissue_type="Brain, glioblastoma" /clone_lib="NIH_MGC_57" /lab_host="DH10B" /note="Vector: pDNR-LIB" gene 3006..4424 /dnas_title="ALG10" /gene="ALG10" /gene_synonym="DIE2" /gene_synonym="KCR1" /db_xref="GeneID:84920" /db_xref="HGNC:23162" /db_xref="MIM:603313" CDS 3006..4424 /dnas_title="ALG10 protein" /gene="ALG10" /gene_synonym="DIE2" /gene_synonym="KCR1" /codon_start=1 /product="ALG10 protein" /protein_id="AAH70347.1" /db_xref="GI:47125451" /db_xref="GeneID:84920" /db_xref="HGNC:23162" /db_xref="MIM:603313" /translation="MAQLEGYYFSAALSCTFLVSCLLFSAFSRALREPYMDEIFHLPQ AQRYCEGHFSLSQWDPMITTLPGLYLVSIGVIKPAIWIFGWSEHVVCSIGMLRFVNLL FSVGNFYLLYLLFCKVQPRNKAASSIQRVLSTLTLAVFPTLYFFNFLYYTEAGSMFFT LFAYLMCLYGNHKTSAFLGFCGFVFRQTNIIWAVFCAGNVIAQKLTEAWKTELQKKED RLPPIKGPFAEFRKILQFLLAYSMSFKNLSMLLLLTWPYILLGFLFCAFVVVNGGIVI GDRSSHEACLHFPQLFYFFSFTLFFSFPHLLSPSKIKTFLSLVWKRRILFFVVTLVSV FLVWKFTYAHKYLLADNRHYTFYVWKRVFQRYETVKYLLVPAYIFAGWSIADSLKSKS IFWNLMFFICLFTVIVPQKLLEFRYFILPYVIYRLNIPLPPTSRLICELSCYAVVNFI TFFIFLNKTFQWPNSQDIQRFMW" misc_feature 3006..4424 /note="ALG10 coding region" misc_difference 3555..3555 /dnas_title="ALG10" /gene="ALG10" /gene_synonym="DIE2" /gene_synonym="KCR1" /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'M' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 4425..4445 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4454) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4454) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4455..6640,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4454 /source="ALG10-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggc gcagctggaa ggttactatt tctcggccgc cttgagctgt acctttttag 3061 tatcctgcct cctcttctcc gccttcagcc gggcgttgcg agagccctac atggacgaga 3121 tcttccacct gcctcaggcg cagcgctact gtgagggcca tttctccctt tcccagtggg 3181 atcccatgat tactacatta cctggcttgt acctggtgtc aattggagtg atcaaacctg 3241 ccatttggat ctttggatgg tctgaacatg ttgtctgctc cattgggatg ctcagatttg 3301 ttaatcttct cttcagtgtt ggcaacttct atttactata tttgcttttc tgcaaggtac 3361 aacccagaaa caaggctgcc tcaagtatcc agagagtctt gtcaacatta acactagcag 3421 tatttccaac actttatttt tttaacttcc tttattatac agaagcagga tctatgtttt 3481 ttactctttt tgcgtatttg atgtgtcttt atggaaatca taaaacttca gccttccttg 3541 gattttgtgg cttcgtgttt cggcaaacaa atatcatctg ggctgtcttc tgtgcaggaa 3601 atgtcattgc acaaaagtta acggaggctt ggaaaactga gctacaaaag aaggaagaca 3661 gacttccacc tattaaagga ccatttgcag aattcagaaa aattcttcag tttcttttgg 3721 cttattccat gtcctttaaa aacttgagta tgcttttgct tctgacttgg ccctacatcc 3781 ttctgggatt tctgttttgt gcttttgtag tagttaatgg tggaattgtt attggcgatc 3841 ggagtagtca tgaagcctgt cttcattttc ctcaactatt ctactttttt tcatttactc 3901 tctttttttc ctttcctcat ctcctgtctc ctagcaaaat taagactttt ctttccttag 3961 tttggaaacg tagaattctg ttttttgtgg ttaccttagt ctctgtgttt ttagtttgga 4021 aattcactta tgctcataaa tacttgctag cagacaatag acattatact ttctatgtgt 4081 ggaaaagagt ttttcaaaga tatgaaactg taaaatattt gttagttcca gcctatatat 4141 ttgctggttg gagtatagct gactcattga aatcaaagtc aattttttgg aatttaatgt 4201 ttttcatatg cttgttcact gttatagttc ctcagaaact gctggaattt cgttacttca 4261 ttttacctta tgtcatttat aggcttaaca tacctctgcc tcccacatcc agactcattt 4321 gtgaactgag ctgctatgca gttgttaatt tcataacttt tttcatcttt ctgaacaaga 4381 cttttcagtg gccaaatagt caggacattc aaaggtttat gtgggaaaac ttgtactttc 4441 aaggctCCAG CTTTCTTGTA CAAAGTGGTA TGAGCAAGGG CGAGGAACTG TTCACCGGCG 4501 TGGTGCCCAT CCTGGTGGAA CTGGATGGCG ACGTGAACGG CCACAAGTTC TCTGTGCGGG 4561 GAGAGGGCGA AGGCGACGCC ACAAATGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG 4621 GCAAGCTGCC CGTGCCTTGG CCTACCCTCG TGACCACACT GACCTACGGC GTGCAGTGCT 4681 TCAGCAGATA CCCCGACCAT ATGAAGCGGC ACGACTTCTT CAAGAGCGCC ATGCCCGAGG 4741 GCTACGTGCA GGAACGGACC ATCAGCTTCA AGGACGACGG CACCTACAAG ACCAGAGCCG 4801 AAGTGAAGTT CGAGGGCGAC ACCCTCGTGA ACCGGATCGA GCTGAAGGGC ATCGATTTCA 4861 AAGAGGACGG CAACATCCTG GGCCACAAGC TGGAGTACAA CTTCAACAGC CACAACGTGT 4921 ACATCACCGC CGACAAGCAG AAGAACGGCA TCAAGGCCAA CTTCAAGATC CGGCACAACG 4981 TGGAAGATGG CAGCGTGCAG CTGGCCGACC ACTACCAGCA GAACACCCCC ATCGGAGATG 5041 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GAGCGTGCTG AGCAAGGACC 5101 CCAACGAGAA GCGGGACCAC ATGGTGCTGC TGGAATTCGT GACCGCCGCT GGCATCACCC 5161 ACGGCATGAG CGGCCTGAAC GACATCTTCG AGGCCCAGAA AATCGAGTGG CACGAGCACC 5221 ACCATCATCA CCATCACCAC TGATGATTCG AAAAATAATA AGGATCCAAT CAACCTCTGG 5281 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT 5341 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT 5401 TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA 5461 GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG 5521 CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG 5581 AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA 5641 ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA 5701 CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC 5761 TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGA GATCTGCCTC 5821 GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC 5881 CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG 5941 TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA 6001 TTGGGAAGAC AATAGCAGGC ATGCTGGGGA CTCGAGTTAA GGGCGAATTG CCGATTAGGA 6061 TCTTCCTAGA GCATGGCTAC GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAAGG 6121 AACCCCTAGT GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG 6181 GGCGACCAAA GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG 6241 CGCGCAGCCT TAATTAAGAG CTCATGGCGC GCCTAGGCCT TGACGGCCTT CCGCCAATTC 6301 GCCCTATAGT GAGTCGTATT ACGTCGCGCT CACTGGCCGT CGTTTTACAA CGTCGTGACT 6361 GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT 6421 GGCGTAATAG CGAAGAGGCC CGCACCGAAA CGCCCTTCCC AACAGTTGCG CAGCCTGAAT 6481 GGCGAATGGG AGCGCCCTGT AGCGGCCACT CAACCCTATC TCGGTCTATT CTTTTGATTT 6541 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT 6601 TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG //