LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1982..2078) /label=attL2 /note="attL2 (c)" misc_signal complement(2093..2112) /note="T7 Promoter/priming site (c)" primer_bind 2120..2136 /note="M13 Reverse priming site" gene 2249..3058 /note="Kanamycin resistance gene" rep_origin 3179..3852 /note="pUC origin" PCR_frame 669..1982 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2002) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..1999) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(1990..3855,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 1960..1980 /note="TEV site" source 673..1959 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:74914 IMAGE:5727996" /tissue_type="Brain, hippocampus" /clone_lib="NIH_MGC_124" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..1959 /gene="POFUT2" /gene_synonym=FUT13 /db_xref="GeneID:23275" /db_xref="HGNC:14683" /db_xref="MIM:610249" CDS 673..1959 /dnas_title="protein O-fucosyltransferase 2" /gene="POFUT2" /gene_synonym=FUT13 /codon_start=1 /product="protein O-fucosyltransferase 2" /protein_id="AAH64623.1" /db_xref="GI:40555888" /db_xref="GeneID:23275" /db_xref="HGNC:14683" /db_xref="MIM:610249" /translation="MATLSFVFLLLGAVSWPPASASGQEFWPGQSAADILSGAASRRR YLLYDVNPPEGFNLRRDVYIRIASLLKTLLKTEEWVLVLPPWGRLYHWQSPDIHQVRI PWSEFFDLPSLNKNIPVIEYEQFIAESGGPFIDQVYVLQSYAEGWKEGTWEEKVDERP CIDQLLYSQDKHEYYRGWFWGYEETRGLNVSCLSVQGSASIVAPLLLRNTSARSVMLD RAENLLHDHYGGKEYWDTRRSMVFARHLREVGDEFRSRHLNSTDDADRIPFQEDWMKM KVKLGSALGGPYLGVHLRRKDFIWGHRQDVPSLEGAVRKIRSLMKTHRLDKVFVATDA VRKEYEELKKLLPEMVRFEPTWEELELYKDGGVAIIDQWICAHARFFIGTSVSTFSFR IHEEREILGLDPKTTYNRFCGDQEKACEQPTHWKITY" misc_feature 673..1959 /note="POFUT2 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggcgac actcagcttc gtcttcctgc tgctgggggc agtgtcctgg 721 cctccggctt ctgcctccgg ccaggagttc tggcccggac aatcggcggc cgatattctg 781 tcgggggcgg cttcccgcag acggtatctt ctgtatgacg tcaacccccc ggaaggcttc 841 aacctgcgca gggatgtcta tatccgaatc gcctctctcc tgaagactct gctgaagacg 901 gaggagtggg tgcttgtcct gcctccatgg ggccgcctct atcactggca gagtcctgac 961 atccaccagg tccggattcc ctggtctgag ttttttgatc ttccaagtct caataaaaac 1021 atccccgtca tcgagtatga gcagttcatc gcagaatctg gtgggccctt tattgaccag 1081 gtttacgtcc tgcaaagtta cgcagagggg tggaaagaag ggacctggga agagaaggtg 1141 gacgagcggc cgtgtattga tcagctcctg tactcccagg acaagcacga gtactacaga 1201 ggatggtttt ggggttatga ggagaccagg ggtctaaacg tctcctgtct gtccgtccag 1261 ggctcagcct ccatcgtggc gcccctgctg ctgagaaaca catcagcccg gtccgtgatg 1321 ttagacagag ccgagaacct acttcacgac cactatggag ggaaagaata ctgggatacc 1381 cgtcgcagca tggtgtttgc caggcacctg cgggaggtgg gagacgagtt caggagcaga 1441 catctcaact ccacggacga cgcagacagg atccccttcc aggaggactg gatgaagatg 1501 aaggtcaagc tgggctccgc gctagggggc ccctacctgg gagtccacct gagaagaaaa 1561 gatttcatct ggggtcacag acaggatgta cccagtctgg aaggggccgt gaggaagatc 1621 cgcagcctca tgaagaccca ccggctggac aaggtgtttg tggccacaga tgccgtcaga 1681 aaggaatatg aagagctaaa aaagctgtta cccgagatgg tgaggtttga acccacgtgg 1741 gaggagctgg agctctacaa ggacggaggc gttgcgatta ttgaccagtg gatctgcgca 1801 cacgccaggt tttttattgg cacctcagtc tcaacatttt cttttcggat tcatgaggaa 1861 agagaaatcc tggggttgga ccccaagacg acgtacaaca ggttctgcgg agaccaagag 1921 aaggcgtgtg agcaacccac ccactggaag atcacctacg aaaacttgta ctttcaaggc 1981 tCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA 2041 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA 2101 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA 2161 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC 2221 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC 2281 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT 2341 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT 2401 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA 2461 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA 2521 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA 2581 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC 2641 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA 2701 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG 2761 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT 2821 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG 2881 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG 2941 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT 3001 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC 3061 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC 3121 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC 3181 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 3241 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 3301 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 3361 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 3421 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 3481 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 3541 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 3601 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 3661 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 3721 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 3781 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 3841 TTTTGCTCAC ATGTT //