LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1922..2018) /label=attL2 /note="attL2 (c)" misc_signal complement(2033..2052) /note="T7 Promoter/priming site (c)" primer_bind 2060..2076 /note="M13 Reverse priming site" gene 2189..2998 /note="Kanamycin resistance gene" rep_origin 3119..3792 /note="pUC origin" vector join(1930..3795,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1915 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:74914 IMAGE:5727996" /tissue_type="Brain, hippocampus" /clone_lib="NIH_MGC_124" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..1915 /gene="POFUT2" /gene_synonym=FUT13 /db_xref="GeneID:23275" /db_xref="HGNC:14683" /db_xref="MIM:610249" CDS 692..1915 /dnas_title="protein O-fucosyltransferase 2" /gene="POFUT2" /gene_synonym=FUT13 /codon_start=1 /product="protein O-fucosyltransferase 2" /protein_id="AAH64623.1" /db_xref="GI:40555888" /db_xref="GeneID:23275" /db_xref="HGNC:14683" /db_xref="MIM:610249" /translation="MATLSFVFLLLGAVSWPPASASGQEFWPGQSAADILSGAASRRR YLLYDVNPPEGFNLRRDVYIRIASLLKTLLKTEEWVLVLPPWGRLYHWQSPDIHQVRI PWSEFFDLPSLNKNIPVIEYEQFIAESGGPFIDQVYVLQSYAEGWKEGTWEEKVDERP CIDQLLYSQDKHEYYRGWFWGYEETRGLNVSCLSVQGSASIVAPLLLRNTSARSVMLD RAENLLHDHYGGKEYWDTRRSMVFARHLREVGDEFRSRHLNSTDDADRIPFQEDWMKM KVKLGSALGGPYLGVHLRRKDFIWGHRQDVPSLEGAVRKIRSLMKTHRLDKVFVATDA VRKEYEELKKLLPEMVRFEPTWEELELYKDGGVAIIDQWICAHARFFIGTSVSTFSFR IHEEREILGLDPKTTYNRFCGDQEKACEQPTHWKITY" misc_feature 692..1915 /note="POFUT2 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ctccggccag gagttctggc ccggacaatc 721 ggcggccgat attctgtcgg gggcggcttc ccgcagacgg tatcttctgt atgacgtcaa 781 ccccccggaa ggcttcaacc tgcgcaggga tgtctatatc cgaatcgcct ctctcctgaa 841 gactctgctg aagacggagg agtgggtgct tgtcctgcct ccatggggcc gcctctatca 901 ctggcagagt cctgacatcc accaggtccg gattccctgg tctgagtttt ttgatcttcc 961 aagtctcaat aaaaacatcc ccgtcatcga gtatgagcag ttcatcgcag aatctggtgg 1021 gccctttatt gaccaggttt acgtcctgca aagttacgca gaggggtgga aagaagggac 1081 ctgggaagag aaggtggacg agcggccgtg tattgatcag ctcctgtact cccaggacaa 1141 gcacgagtac tacagaggat ggttttgggg ttatgaggag accaggggtc taaacgtctc 1201 ctgtctgtcc gtccagggct cagcctccat cgtggcgccc ctgctgctga gaaacacatc 1261 agcccggtcc gtgatgttag acagagccga gaacctactt cacgaccact atggagggaa 1321 agaatactgg gatacccgtc gcagcatggt gtttgccagg cacctgcggg aggtgggaga 1381 cgagttcagg agcagacatc tcaactccac ggacgacgca gacaggatcc ccttccagga 1441 ggactggatg aagatgaagg tcaagctggg ctccgcgcta gggggcccct acctgggagt 1501 ccacctgaga agaaaagatt tcatctgggg tcacagacag gatgtaccca gtctggaagg 1561 ggccgtgagg aagatccgca gcctcatgaa gacccaccgg ctggacaagg tgtttgtggc 1621 cacagatgcc gtcagaaagg aatatgaaga gctaaaaaag ctgttacccg agatggtgag 1681 gtttgaaccc acgtgggagg agctggagct ctacaaggac ggaggcgttg cgattattga 1741 ccagtggatc tgcgcacacg ccaggttttt tattggcacc tcagtctcaa cattttcttt 1801 tcggattcat gaggaaagag aaatcctggg gttggacccc aagacgacgt acaacaggtt 1861 ctgcggagac caagagaagg cgtgtgagca acccacccac tggaagatca cctacTAGGA 1921 CCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA 1981 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA 2041 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA 2101 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC 2161 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC 2221 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT 2281 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT 2341 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA 2401 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA 2461 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA 2521 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC 2581 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA 2641 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG 2701 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT 2761 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG 2821 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG 2881 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT 2941 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC 3001 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC 3061 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC 3121 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 3181 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 3241 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 3301 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 3361 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 3421 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 3481 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 3541 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 3601 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 3661 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 3721 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 3781 TTTTGCTCAC ATGTT //