LOCUS PIGV-pGEc2-DEST 6700 bp DNA circular 23-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6178..6307) /note="3'-ITR" misc_feature complement(5876..6090) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5328..5869 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4507..4529 /note="attB2" misc_feature 5307..5312 /note="BstB1 site" misc_feature 4530..5225 /note="Super GFP" misc_feature 5226..5276 /note="AviTag" misc_feature 5277..5300 /note="His Tag" PCR_frame 3002..4507 /note="Reading Frame" /set="Set 1" source 3006..4484 /dnas_title="PIGV-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:8856 IMAGE:3890907" /tissue_type="Lung, carcinoma, large cell undifferentiated." /clone_lib="NIH_MGC_69" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..4484 /dnas_title="PIGV" /gene="PIGV" /gene_synonym="FLJ20477" /gene_synonym="RP1-50O24.2" /db_xref="GeneID:55650" /db_xref="HGNC:26031" CDS 3006..4484 /dnas_title="phosphatidylinositol glycan anchor biosynthesis, class V" /gene="PIGV" /gene_synonym="FLJ20477" /gene_synonym="RP1-50O24.2" /codon_start=1 /product="phosphatidylinositol glycan anchor biosynthesis, class V" /protein_id="AAH13568.1" /db_xref="GI:15488876" /db_xref="GeneID:55650" /db_xref="HGNC:26031" /translation="MWPQDPSRKEVLRFAVSCRILTLMLQALFNAIIPDHHAEAFSPP RLAPSGFVDQLVEGLLGGLSHWDAEHFLFIAEHGYLYEHNFAFFPGFPLALLVGTELL RPLRGLLSLRSCLLISVASLNFLFFMLAAVALHDLGCLVLHCPHQSFYAALLFCLSPA NVFLAAGYSEALFALLTFSAMGQLERGRVWTSVLLFAFATGVRSNGLVSVGFLMHSQC QGFFSSLTMLNPLRQLFKLMASLFLSVFTLGLPFALFQYYAYTQFCLPGSARPIPEPL VQLAVDKGYRIAEGNEPPWCFWDVPLIYSYIQDVYWNVGFLKYYELKQVPNFLLAAPV AILVAWATWTYVTTHPWLCLTLGLQRSKNNKTLEKPDLGFLSPQVFVYVVHAAVLLLF GGLCMHVQVLTRFLGSSTPIMYWFPAHLLQDQEPLLRSLKTVPWKPLAEDSPPGQKVP RNPIMGLLYHWKTCSPVTRYILGYFLTYWLLGLLLHCNFLPWT" misc_feature 3006..4484 /note="PIGV coding region" misc_feature 4485..4505 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4514) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4514) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4515..6700,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4514 /source="PIGV-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgtg gccccaggac ccatcccgga aggaggtgct gaggtttgca gtcagctgcc 3061 gtatcctgac tctgatgctg caggccctct tcaatgccat catcccagat caccatgcag 3121 aagccttctc tcctcctcgc ctggccccct caggctttgt ggaccaactc gtggaaggtc 3181 ttctgggcgg cctgtctcac tgggatgctg aacacttctt gttcattgct gagcatggct 3241 acctgtatga gcacaacttt gccttctttc ctggtttccc cttggccctg ctggtgggga 3301 ctgaactgtt gagaccctta cgggggttac tgagtctacg cagttgcctg ctgatttcgg 3361 tagcatcact caatttcttg ttcttcatgt tggctgcagt tgcacttcat gacctgggtt 3421 gtctggtttt gcactgtccc caccagtcct tttatgcagc tctgcttttc tgtctcagcc 3481 ctgccaatgt cttcctggca gctggttact cagaagcttt gtttgccctc ctgacattca 3541 gtgccatggg gcagctggag aggggccgag tctggactag tgtactcctc tttgcctttg 3601 ccactggggt acgctccaac gggctggtca gtgttggctt cctcatgcat tctcaatgcc 3661 aaggcttttt ctcttctcta acgatgctga atcctctgag acagctcttt aagctgatgg 3721 cctctctgtt tctgtcggtg ttcacacttg gccttccctt tgccctcttt cagtattatg 3781 cctacaccca attctgtctg ccaggctcag cccgccccat tcctgagcct ttggtacagt 3841 tagctgtaga caagggctac cggattgcag agggaaatga accgccttgg tgcttctggg 3901 atgttccact aatatacagc tatatccagg atgtctactg gaatgttggc tttttgaaat 3961 actatgagct caagcaggtg cccaattttc tactggctgc accagtggct atactggttg 4021 cctgggcaac ttggacatac gtgaccactc acccttggct ctgccttaca cttgggctgc 4081 aaaggagcaa gaacaataag accctagaga agcccgatct tggattcctc agtcctcagg 4141 tgtttgtgta cgtggtccac gctgcagtgc tgctgctgtt tggaggtctg tgcatgcatg 4201 ttcaggttct caccaggttt ttgggctcct ccactcctat tatgtactgg tttccagctc 4261 acttgcttca ggatcaagag ccgctgttga gatccttaaa gactgtgcct tggaagcctc 4321 ttgcagagga ctccccacca ggacaaaagg tccccagaaa tcctatcatg ggacttttgt 4381 atcactggaa aacctgttct ccagtcacac gatacattct aggctacttc ctgacttact 4441 ggctcctggg actactccta cattgcaact tcctgccttg gacagaaaac ttgtactttc 4501 aaggctCCAG CTTTCTTGTA CAAAGTGGTA TGAGCAAGGG CGAGGAACTG TTCACCGGCG 4561 TGGTGCCCAT CCTGGTGGAA CTGGATGGCG ACGTGAACGG CCACAAGTTC TCTGTGCGGG 4621 GAGAGGGCGA AGGCGACGCC ACAAATGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG 4681 GCAAGCTGCC CGTGCCTTGG CCTACCCTCG TGACCACACT GACCTACGGC GTGCAGTGCT 4741 TCAGCAGATA CCCCGACCAT ATGAAGCGGC ACGACTTCTT CAAGAGCGCC ATGCCCGAGG 4801 GCTACGTGCA GGAACGGACC ATCAGCTTCA AGGACGACGG CACCTACAAG ACCAGAGCCG 4861 AAGTGAAGTT CGAGGGCGAC ACCCTCGTGA ACCGGATCGA GCTGAAGGGC ATCGATTTCA 4921 AAGAGGACGG CAACATCCTG GGCCACAAGC TGGAGTACAA CTTCAACAGC CACAACGTGT 4981 ACATCACCGC CGACAAGCAG AAGAACGGCA TCAAGGCCAA CTTCAAGATC CGGCACAACG 5041 TGGAAGATGG CAGCGTGCAG CTGGCCGACC ACTACCAGCA GAACACCCCC ATCGGAGATG 5101 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GAGCGTGCTG AGCAAGGACC 5161 CCAACGAGAA GCGGGACCAC ATGGTGCTGC TGGAATTCGT GACCGCCGCT GGCATCACCC 5221 ACGGCATGAG CGGCCTGAAC GACATCTTCG AGGCCCAGAA AATCGAGTGG CACGAGCACC 5281 ACCATCATCA CCATCACCAC TGATGATTCG AAAAATAATA AGGATCCAAT CAACCTCTGG 5341 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT 5401 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT 5461 TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA 5521 GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG 5581 CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG 5641 AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA 5701 ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA 5761 CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC 5821 TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGA GATCTGCCTC 5881 GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC 5941 CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG 6001 TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA 6061 TTGGGAAGAC AATAGCAGGC ATGCTGGGGA CTCGAGTTAA GGGCGAATTG CCGATTAGGA 6121 TCTTCCTAGA GCATGGCTAC GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAAGG 6181 AACCCCTAGT GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG 6241 GGCGACCAAA GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG 6301 CGCGCAGCCT TAATTAAGAG CTCATGGCGC GCCTAGGCCT TGACGGCCTT CCGCCAATTC 6361 GCCCTATAGT GAGTCGTATT ACGTCGCGCT CACTGGCCGT CGTTTTACAA CGTCGTGACT 6421 GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT 6481 GGCGTAATAG CGAAGAGGCC CGCACCGAAA CGCCCTTCCC AACAGTTGCG CAGCCTGAAT 6541 GGCGAATGGG AGCGCCCTGT AGCGGCCACT CAACCCTATC TCGGTCTATT CTTTTGATTT 6601 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT 6661 TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG //