LOCUS KDELC1-pGEc2-DES 6715 bp DNA circular 23-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6193..6322) /note="3'-ITR" misc_feature complement(5891..6105) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5343..5884 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4522..4544 /note="attB2" misc_feature 5322..5327 /note="BstB1 site" misc_feature 4545..5240 /note="Super GFP" misc_feature 5241..5291 /note="AviTag" misc_feature 5292..5315 /note="His Tag" PCR_frame 3002..4522 /note="Reading Frame" /set="Set 1" source 3006..4499 /dnas_title="KDELC1-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:5302 IMAGE:3456869" /tissue_type="Cervix, carcinoma" /clone_lib="NIH_MGC_12" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..4499 /dnas_title="KDELC1" /gene="KDELC1" /gene_synonym="EP58" /gene_synonym="KDEL1" /gene_synonym="MGC5302" /db_xref="GeneID:79070" /db_xref="HGNC:19350" CDS 3006..4499 /dnas_title="KDEL (Lys-Asp-Glu-Leu) containing 1" /gene="KDELC1" /gene_synonym="EP58" /gene_synonym="KDEL1" /gene_synonym="MGC5302" /codon_start=1 /product="KDEL (Lys-Asp-Glu-Leu) containing 1" /protein_id="AAH01297.1" /db_xref="GI:12654903" /db_xref="GeneID:79070" /db_xref="HGNC:19350" /translation="MFGTLLLYCFFLATVPALAETGGERQLSPEKSEIWGPGLKADVV LPARYFYIQAVDTSGNKFTSSPGEKVFQVKVSAPEEQFTRVGVQVLDRKDGSFIVRYR MYASYKNLKVEVKFQGQHVAKSPYILKGPVYHENCDCPLQDSAAWLREMNCPETIAQI QRDLAHFPAVDPEKIAVEIPKRFGQRQSLCHYTLKDNKVYIKTHGEHVGFRIFMDAIL LSLTRKVKMPDVELFVNLGDWPLEKKKSNSNIHPIFSWCGSTDSKDIVMPTYDLTDSV LETMGRVSLDMMSVQANTGPPWESKNSTAVWRGRDSRKERLELVKLSRKHPELIDAAF TNFFFFKHDENLYGPIVKHISFFDFFKHKYQINIDGTVAAYRLPYLLVGDSVVLKQDS IYYEHFYNELQPWKHYIPVKSNLSDLLEKLKWAKDHDEEAKKIAKAGQEFARNNLMGD DIFCYYFKLFQEYANLQVSEPQIREGMKRVEPQTEDDLFPCTCHRKKTKDEL" misc_feature 3006..4499 /note="KDELC1 coding region" misc_difference 3345..3345 /dnas_title="KDELC1" /gene="KDELC1" /gene_synonym="EP58" /gene_synonym="KDEL1" /gene_synonym="MGC5302" /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'I' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 4500..4520 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4529) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4529) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4530..6715,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4529 /source="KDELC1-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgtt tggcactttg ctactttatt gcttctttct ggcgacagtt ccagcactcg 3061 ccgagaccgg cggagaaagg cagctgagcc cggagaagag cgaaatatgg ggacccgggc 3121 taaaagcaga cgtcgtcctt cccgcccgct atttctatat tcaggcagtg gatacatcag 3181 ggaataaatt cacatcttct ccaggcgaaa aggtcttcca ggtgaaagtc tcagcaccag 3241 aggagcaatt cactagagtt ggagtccagg ttttagaccg aaaagatggg tccttcatag 3301 taagatacag aatgtatgca agctacaaaa atctgaaggt ggaagttaaa ttccaagggc 3361 aacatgtggc caaatcccca tatattttaa aagggccggt ttaccatgag aactgtgact 3421 gtcctctgca agatagtgca gcctggctac gggagatgaa ctgccctgaa accattgctc 3481 agattcagag agatctggca catttccctg ctgtggatcc agaaaagatt gcagtagaaa 3541 tcccaaaaag atttggacag aggcagagcc tatgtcacta caccttaaag gataacaagg 3601 tttatatcaa gactcatggt gaacatgtag gttttagaat tttcatggat gccatactac 3661 tttctttgac tagaaaggtg aagatgccag atgtggagct ctttgttaat ttgggagact 3721 ggcctttgga aaaaaagaaa tccaattcaa acatccatcc gatcttttcc tggtgtggct 3781 ccacagattc caaggatatc gtgatgccta cgtacgattt gactgattct gttctggaaa 3841 ccatgggccg ggtaagtctg gatatgatgt ccgtgcaagc taacacgggt cctccctggg 3901 aaagcaaaaa ttccactgcc gtctggagag ggcgagacag ccgcaaagag agactcgagc 3961 tggttaaact cagtagaaaa cacccagaac tcatagacgc tgctttcacc aactttttct 4021 tctttaaaca cgatgaaaac ctgtatggtc ccattgtgaa acatatttca ttttttgatt 4081 tcttcaagca taagtatcaa ataaatatcg atggcactgt agcagcttat cgcctgccat 4141 atttgctagt tggtgacagt gttgtgctga agcaggattc catctactat gaacattttt 4201 acaatgagct gcagccctgg aaacactaca ttccagttaa gagcaacctg agcgatctgc 4261 tagaaaaact taaatgggcg aaagatcacg atgaagaggc caaaaagata gcaaaagcag 4321 gacaagaatt tgcaagaaat aatctcatgg gcgatgacat attctgttat tatttcaaac 4381 ttttccagga atatgccaat ttacaagtga gtgagcccca aatccgagag ggcatgaaaa 4441 gggtagaacc acagactgag gacgacctct tcccttgtac ttgccatagg aaaaagaccg 4501 aaaacttgta ctttcaaggc tCCAGCTTTC TTGTACAAAG TGGTATGAGC AAGGGCGAGG 4561 AACTGTTCAC CGGCGTGGTG CCCATCCTGG TGGAACTGGA TGGCGACGTG AACGGCCACA 4621 AGTTCTCTGT GCGGGGAGAG GGCGAAGGCG ACGCCACAAA TGGCAAGCTG ACCCTGAAGT 4681 TCATCTGCAC CACCGGCAAG CTGCCCGTGC CTTGGCCTAC CCTCGTGACC ACACTGACCT 4741 ACGGCGTGCA GTGCTTCAGC AGATACCCCG ACCATATGAA GCGGCACGAC TTCTTCAAGA 4801 GCGCCATGCC CGAGGGCTAC GTGCAGGAAC GGACCATCAG CTTCAAGGAC GACGGCACCT 4861 ACAAGACCAG AGCCGAAGTG AAGTTCGAGG GCGACACCCT CGTGAACCGG ATCGAGCTGA 4921 AGGGCATCGA TTTCAAAGAG GACGGCAACA TCCTGGGCCA CAAGCTGGAG TACAACTTCA 4981 ACAGCCACAA CGTGTACATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA 5041 AGATCCGGCA CAACGTGGAA GATGGCAGCG TGCAGCTGGC CGACCACTAC CAGCAGAACA 5101 CCCCCATCGG AGATGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGAGCG 5161 TGCTGAGCAA GGACCCCAAC GAGAAGCGGG ACCACATGGT GCTGCTGGAA TTCGTGACCG 5221 CCGCTGGCAT CACCCACGGC ATGAGCGGCC TGAACGACAT CTTCGAGGCC CAGAAAATCG 5281 AGTGGCACGA GCACCACCAT CATCACCATC ACCACTGATG ATTCGAAAAA TAATAAGGAT 5341 CCAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG 5401 CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC 5461 GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT 5521 TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA 5581 CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC 5641 CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC 5701 TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC 5761 TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC 5821 TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC 5881 TTCGAGATCT GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC 5941 CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA 6001 AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA 6061 CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGACTCGA GTTAAGGGCG 6121 AATTGCCGAT TAGGATCTTC CTAGAGCATG GCTACGTAGA TAAGTAGCAT GGCGGGTTAA 6181 TCATTAACTA CAAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT 6241 CGCTCACTGA GGCCGGGCGA CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT 6301 CAGTGAGCGA GCGAGCGCGC AGCCTTAATT AAGAGCTCAT GGCGCGCCTA GGCCTTGACG 6361 GCCTTCCGCC AATTCGCCCT ATAGTGAGTC GTATTACGTC GCGCTCACTG GCCGTCGTTT 6421 TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC 6481 CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGAAACGCCC TTCCCAACAG 6541 TTGCGCAGCC TGAATGGCGA ATGGGAGCGC CCTGTAGCGG CCACTCAACC CTATCTCGGT 6601 CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT 6661 GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAG //