LOCUS AGA-pGEc2 6264 bp DNA circular 21-SEP-2016 FEATURES Location/Qualifiers misc_feature complement(5742..5871) /note="3'-ITR" misc_feature complement(5440..5654) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 4892..5433 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4071..4093 /note="attB2" misc_feature 4871..4876 /note="BstB1 site" misc_feature 4094..4789 /note="Super GFP" misc_feature 4790..4840 /note="AviTag" misc_feature 4841..4864 /note="His Tag" PCR_frame 3011..4070 /note="Reading Frame" /set="Set 1" misc_feature 3011..4048 /note="OPTIMIZED AGA coding region" misc_feature 4049..4070 /note="TEV" vector join(4079..6264,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4078 /source="AGA pDONR" /type="Entry Clone" source 1..6264 /dnas_title="AGA-pGEc2" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCGCCACC ATGGCCAGAA AGTCTAATCT GCCTGTGCTG CTGGTGCCCT TCCTGCTGTG 3061 TCAGGCTCTC GTCAGATGTA GCTCTCCTCT GCCCCTGGTG GTCAACACCT GGCCTTTTAA 3121 GAATGCCACC GAGGCCGCTT GGAGAGCACT GGCTAGCGGA GGATCTGCTC TGGATGCCGT 3181 GGAATCTGGA TGTGCCATGT GCGAGAGGGA ACAGTGTGAT GGCTCTGTGG GCTTTGGCGG 3241 CTCTCCTGAT GAGCTGGGAG AGACAACACT GGACGCCATG ATTATGGACG GCACCACAAT 3301 GGATGTGGGC GCCGTGGGAG ATCTGCGGAG AATCAAAAAT GCCATCGGCG TGGCCCGGAA 3361 GGTGCTGGAA CATACAACAC ACACACTGCT CGTGGGCGAG AGCGCCACAA CATTTGCCCA 3421 GAGCATGGGC TTCATCAACG AGGACCTGAG CACAACAGCC AGCCAGGCCC TGCATTCTGA 3481 TTGGCTGGCA AGAAACTGCC AGCCCAACTA CTGGCGGAAC GTGATCCCCG ATCCTAGCAA 3541 GTACTGCGGC CCTTACAAGC CCCCTGGCAT CCTGAAGCAG GACATCCCCA TCCACAAAGA 3601 GACAGAGGAC GACAGAGGCC ACGACACCAT CGGAATGGTC GTGATCCACA AGACCGGACA 3661 CATTGCCGCC GGAACCAGCA CCAACGGCAT CAAGTTCAAG ATCCACGGAA GAGTGGGCGA 3721 CAGCCCTATT CCTGGCGCTG GCGCTTATGC TGATGATACA GCTGGTGCTG CCGCCGCTAC 3781 AGGCAATGGC GATATTCTGA TGCGGTTCCT GCCTAGCTAC CAGGCCGTCG AGTATATGAG 3841 AAGAGGCGAG GACCCTACAA TCGCCTGTCA GAAAGTGATC AGCCGGATCC AGAAGCACTT 3901 CCCCGAGTTC TTCGGAGCCG TGATCTGTGC CAATGTGACA GGATCTTACG GCGCTGCCTG 3961 CAACAAGCTG TCCACCTTCA CCCAGTTCAG CTTCATGGTG TACAACAGCG AGAAGAACCA 4021 GCCCACCGAA GAGAAAGTGG ACTGCATCga aaacttgtac tttcaaggct CCAGCTTTCT 4081 TGTACAAAGT GGTATGAGCA AGGGCGAGGA ACTGTTCACC GGCGTGGTGC CCATCCTGGT 4141 GGAACTGGAT GGCGACGTGA ACGGCCACAA GTTCTCTGTG CGGGGAGAGG GCGAAGGCGA 4201 CGCCACAAAT GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC 4261 TTGGCCTACC CTCGTGACCA CACTGACCTA CGGCGTGCAG TGCTTCAGCA GATACCCCGA 4321 CCATATGAAG CGGCACGACT TCTTCAAGAG CGCCATGCCC GAGGGCTACG TGCAGGAACG 4381 GACCATCAGC TTCAAGGACG ACGGCACCTA CAAGACCAGA GCCGAAGTGA AGTTCGAGGG 4441 CGACACCCTC GTGAACCGGA TCGAGCTGAA GGGCATCGAT TTCAAAGAGG ACGGCAACAT 4501 CCTGGGCCAC AAGCTGGAGT ACAACTTCAA CAGCCACAAC GTGTACATCA CCGCCGACAA 4561 GCAGAAGAAC GGCATCAAGG CCAACTTCAA GATCCGGCAC AACGTGGAAG ATGGCAGCGT 4621 GCAGCTGGCC GACCACTACC AGCAGAACAC CCCCATCGGA GATGGCCCCG TGCTGCTGCC 4681 CGACAACCAC TACCTGAGCA CCCAGAGCGT GCTGAGCAAG GACCCCAACG AGAAGCGGGA 4741 CCACATGGTG CTGCTGGAAT TCGTGACCGC CGCTGGCATC ACCCACGGCA TGAGCGGCCT 4801 GAACGACATC TTCGAGGCCC AGAAAATCGA GTGGCACGAG CACCACCATC ATCACCATCA 4861 CCACTGATGA TTCGAAAAAT AATAAGGATC CAATCAACCT CTGGATTACA AAATTTGTGA 4921 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT 4981 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA 5041 ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT 5101 GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT 5161 CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG 5221 CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC 5281 GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG 5341 GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT 5401 GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGAGATCTG CCTCGACTGT GCCTTCTAGT 5461 TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT 5521 CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT 5581 TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC 5641 AGGCATGCTG GGGACTCGAG TTAAGGGCGA ATTGCCGATT AGGATCTTCC TAGAGCATGG 5701 CTACGTAGAT AAGTAGCATG GCGGGTTAAT CATTAACTAC AAGGAACCCC TAGTGATGGA 5761 GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC 5821 CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCCTTAATTA 5881 AGAGCTCATG GCGCGCCTAG GCCTTGACGG CCTTCCGCCA ATTCGCCCTA TAGTGAGTCG 5941 TATTACGTCG CGCTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT 6001 TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA 6061 GGCCCGCACC GAAACGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGGAGCGCC 6121 CTGTAGCGGC CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 6181 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC 6241 AAAATATTAA CGCTTACAAT TTAG //