LOCUS ISPD-pGEc1 5771 bp DNA circular 22-SEP-2016 FEATURES Location/Qualifiers misc_feature complement(5249..5378) /note="3'-ITR" misc_feature complement(4947..5161) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 4399..4940 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_recomb 4304..4326 /label=attB2 /note="attB2 site" misc_feature 4336..4359 /note="8xHis Tag" misc_feature 4363..4386 /note="StrepTag" CDS 3011..4300 /label=ORF1 misc_feature 3005..3010 /note="Kozak seq" misc_feature 3017..3040 /note="His Tag" misc_feature 3047..3070 /note="TEV protease site" misc_feature 3071..4302 /note="ISPD coding region" vector join(4312..5771,1..2991) /source="pGEc1-DEST" /type="Destination Vector" clone 2992..4311 /source="OPTIMIZED ISPD coding casette" /type="Entry Clone" source 1..5771 /dnas_title="ISPD-pGEc1" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCGCCACC ATGGCCCACC ACCATCATCA CCATCACCAC GAGTTCGAGA ACCTGTACTT 3061 CCAGAGCGGC ATGCACCCTC AGGCCGTGGC TGCTGTGCTG CCTGCTGGCG GATGTGGCGA 3121 GAGAATGGGC GTGCCAACCC CCAAGCAGTT CTGCCCCATC CTGGAACGGC CCCTGATCAG 3181 CTACACACTG CAGGCCCTGG AAAGAGTGTG CTGGATCAAG GACATCGTGG TGGCCGTGAC 3241 CGGCGAGAAC ATGGAAGTGA TGAAGTCCAT CATCCAGAAG TACCAGCACA AGCGGATCAG 3301 CCTGGTGGAA GCCGGCGTGA CCAGACACCG GTCCATCTTC AATGGCCTGA AGGCCCTGGC 3361 CGAGGACCAG ATCAACAGCA AGCTGAGCAA GCCCGAGGTC GTGATCATCC ACGATGCCGT 3421 GCGGCCCTTC GTGGAAGAAG GCGTGCTGCT GAAGGTCGTG ACCGCCGCCA AAGAACATGG 3481 CGCCGCTGGC GCTATCAGAC CCCTGGTGTC TACCGTGGTG TCCCCTAGCG CCGATGGCTG 3541 CCTGGACTAC AGCCTGGAAA GGGCCAGACA CAGAGCCAGC GAGATGCCCC AGGCCTTCCT 3601 GTTCGACGTG ATCTACGAGG CCTACCAGCA GTGCAGCGAC TACGACCTGG AATTCGGCAC 3661 CGAGTGCCTG CAGCTGGCCC TGAAGTACTG CTGCACCAAG GCCAAGCTGG TGGAAGGCAG 3721 CCCCGACCTG TGGAAAGTGA CCTACAAGCG GGACCTGTAC GCCGCCGAGA GCATCATCAA 3781 AGAGAGAATC AGCCAGGAAA TCTGTGTCGT GATGGACACC GAAGAGGACA ACAAGCACGT 3841 GGGCCATCTG CTGGAAGAGG TGCTGAAGTC CGAGCTGAAC CACGTGAAAG TGACAAGCGA 3901 GGCCCTGGGC CACGCCGGCA GACATCTGCA GCAGATCATC CTGGACCAGT GCTACAACTT 3961 CGTGTGCGTG AACGTGACCA CCAGCGACTT CCAGGAAACC CAGAAACTGC TGAGCATGCT 4021 GGAAGAAAGC AGCCTGTGCA TCCTGTACCC CGTGGTGGTG GTGTCCGTGC ACTTCCTGGA 4081 CTTTAAGCTG GTGCCCCCCA GCCAGAAAAT GGAAAACCTG ATGCAGATCC GCGAGTTTGC 4141 CAAAGAAGTG AAAGAGCGGA ACATCCTGCT GTACGGACTG CTGATTAGCT ACCCCCAGGA 4201 CGACCAGAAG CTGCAGGAAA GCCTGAGACA GGGCGCCATC ATCATTGCCA GCCTGATCAA 4261 AGAACGGAAC AGCGGCCTGA TCGGACAGCT GCTGATTGCC TGACCAGCTT TCTTGTACAA 4321 AGTGGTGATG GCCGCCATCA TCATCATCAT CATCATCATG CGTGGAGCCA CCCGCAGTTC 4381 GAAAAATAAT AAGGATCCAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT 4441 ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT 4501 CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG 4561 TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT 4621 GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT 4681 TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC 4741 TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG 4801 TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC 4861 TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG 4921 CGGCCTCTTC CGCGTCTTCG AGATCTGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG 4981 TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT 5041 CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG 5101 GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG 5161 ACTCGAGTTA AGGGCGAATT GCCGATTAGG ATCTTCCTAG AGCATGGCTA CGTAGATAAG 5221 TAGCATGGCG GGTTAATCAT TAACTACAAG GAACCCCTAG TGATGGAGTT GGCCACTCCC 5281 TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA AGGTCGCCCG ACGCCCGGGC 5341 TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCC TTAATTAAGA GCTCATGGCG 5401 CGCCTAGGCC TTGACGGCCT TCCGCCAATT CGCCCTATAG TGAGTCGTAT TACGTCGCGC 5461 TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT 5521 CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAA 5581 ACGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG GAGCGCCCTG TAGCGGCCAC 5641 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 5701 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC 5761 TTACAATTTA G //