LOCUS HSPA5-pGEn1-DEST 6490 bp DNA circular 26-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 5118..5659 /note="WPRE" misc_recomb 5080..5102 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(5666..5880) /note="BGH pA" misc_feature complement(5968..6097) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..5073 /dnas_title="HSPA5-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:31939 IMAGE:5020098" /tissue_type="Muscle, rhabdomyosarcoma" /clone_lib="NIH_MGC_17" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3166..5073 /dnas_title="HSPA5" /gene="HSPA5" /gene_synonym="BIP" /gene_synonym="MIF2" /db_xref="GeneID:3309" /db_xref="HGNC:5238" /db_xref="MIM:138120" CDS 3166..5073 /dnas_title="heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)" /gene="HSPA5" /gene_synonym="BIP" /gene_synonym="MIF2" /codon_start=1 /product="heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)" /protein_id="AAH20235.1" /db_xref="GI:18044381" /db_xref="GeneID:3309" /db_xref="HGNC:5238" /db_xref="MIM:138120" /translation="MKLSLVAAMLLLLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGV FKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRT WNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAY LGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEK NILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGK DVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDL FRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDE AVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQI FSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGI LRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELE SYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELE EIVQPIISKLYGSAGPPPTGEEDTAEKDEL" misc_feature 3166..5073 /note="HSPA5 coding region" vector join(5088..6490,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..5087 /source="HSPA5-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcgagga ggaggacaag 3181 aaggaggacg tgggcacggt ggtcggcatc gacctgggga ccacctactc ctgcgtcggc 3241 gtgttcaaga acggccgcgt ggagatcatc gccaacgatc agggcaaccg catcacgccg 3301 tcctatgtcg ccttcactcc tgaaggggaa cgtctgattg gcgatgccgc caagaaccag 3361 ctcacctcca accccgagaa cacggtcttt gacgccaagc ggctcatcgg ccgcacgtgg 3421 aatgacccgt ctgtgcagca ggacatcaag ttcttgccgt tcaaggtggt tgaaaagaaa 3481 actaaaccat acattcaagt tgatattgga ggtgggcaaa caaagacatt tgctcctgaa 3541 gaaatttctg ccatggttct cactaaaatg aaagaaaccg ctgaggctta tttgggaaag 3601 aaggttaccc atgcagttgt tactgtacca gcctatttta atgatgccca acgccaagca 3661 accaaagacg ctggaactat tgctggccta aatgttatga ggatcatcaa cgagcctacg 3721 gcagctgcta ttgcttatgg cctggataag agggaggggg agaagaacat cctggtgttt 3781 gacctgggtg gcggaacctt cgatgtgtct cttctcacca ttgacaatgg tgtcttcgaa 3841 gttgtggcca ctaatggaga tactcatctg ggtggagaag actttgacca gcgtgtcatg 3901 gaacacttca tcaaactgta caaaaagaag acgggcaaag atgtcaggaa agacaataga 3961 gctgtgcaga aactccggcg cgaggtagaa aaggccaaac gggccctgtc ttctcagcat 4021 caagcaagaa ttgaaattga gtccttctat gaaggagaag acttttctga gaccctgact 4081 cgggccaaat ttgaagagct caacatggat ctgttccggt ctactatgaa gcccgtccag 4141 aaagtgttgg aagattctga tttgaagaag tctgatattg atgaaattgt tcttgttggt 4201 ggctcgactc gaattccaaa gattcagcaa ctggttaaag agttcttcaa tggcaaggaa 4261 ccatcccgtg gcataaaccc agatgaagct gtagcgtatg gtgctgctgt ccaggctggt 4321 gtgctctctg gtgatcaaga tacaggtgac ctggtactgc ttgatgtatg tccccttaca 4381 cttggtattg aaactgtggg aggtgtcatg accaaactga ttccaaggaa cacagtggtg 4441 cctaccaaga agtctcagat cttttctaca gcttctgata atcaaccaac tgttacaatc 4501 aaggtctatg aaggtgaaag acccctgaca aaagacaatc atcttctggg tacatttgat 4561 ctgactggaa ttcctcctgc tcctcgtggg gtcccacaga ttgaagtcac ctttgagata 4621 gatgtgaatg gtattcttcg agtgacagct gaagacaagg gtacagggaa caaaaataag 4681 atcacaatca ccaatgacca gaatcgcctg acacctgaag aaatcgaaag gatggttaat 4741 gatgctgaga agtttgctga ggaagacaaa aagctcaagg agcgcattga tactagaaat 4801 gagttggaaa gctatgccta ttctctaaag aatcagattg gagataaaga aaagctggga 4861 ggtaaacttt cctctgaaga taaggagacc atggaaaaag ctgtagaaga aaagattgaa 4921 tggctggaaa gccaccaaga tgctgacatt gaagacttca aagctaagaa gaaggaactg 4981 gaagaaattg ttcaaccaat tatcagcaaa ctctatggaa gtgcaggccc tcccccaact 5041 ggtgaagagg atacagcaga aaaagatgag ttgTAGGACC CAGCTTTCTT GTACAAAGTG 5101 GTTGATAGCT TGGATCCAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA 5161 TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC 5221 ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT 5281 CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG 5341 CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT 5401 TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT 5461 GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT 5521 CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT 5581 ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC 5641 GGCCTCTTCC GCGTCTTCGA GATCTGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT 5701 TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC 5761 CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG 5821 TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA 5881 CTCGAGTTAA GGGCGAATTG CCGATTAGGA TCTTCCTAGA GCATGGCTAC GTAGATAAGT 5941 AGCATGGCGG GTTAATCATT AACTACAAGG AACCCCTAGT GATGGAGTTG GCCACTCCCT 6001 CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG GGCGACCAAA GGTCGCCCGA CGCCCGGGCT 6061 TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG CGCGCAGCCT TAATTAAGAG CTCATGGCGC 6121 GCCTAGGCCT TGACGGCCTT CCGCCAATTC GCCCTATAGT GAGTCGTATT ACGTCGCGCT 6181 CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC 6241 GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGAAA 6301 CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGG AGCGCCCTGT AGCGGCCACT 6361 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG 6421 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT 6481 TACAATTTAG //