LOCUS PDIA2-pGEc2-DEST 6784 bp DNA circular 26-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6262..6391) /note="3'-ITR" misc_feature complement(5960..6174) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5412..5953 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4591..4613 /note="attB2" misc_feature 5391..5396 /note="BstB1 site" misc_feature 4614..5309 /note="Super GFP" misc_feature 5310..5360 /note="AviTag" misc_feature 5361..5384 /note="His Tag" PCR_frame 3002..4591 /note="Reading Frame" /set="Set 1" source 3006..4568 /dnas_title="PDIA2-pGEc2-DEST" /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="MGC:195833 IMAGE:100068216" /clone_lib="NIH_MGC_435" /lab_host="DH10B" /focus /note="Vector: pENTR223.1 with stop codon" source 3006..4568 /organism="Homo sapiens" /mol_type="other DNA" /db_xref="taxon:9606" gene 3006..4568 /dnas_title="PDIA2" /gene="PDIA2" /gene_synonym="PDA2" /gene_synonym="PDI" /gene_synonym="PDIR" /db_xref="GeneID:64714" /db_xref="HGNC:14180" /db_xref="MIM:608012" CDS 3006..4568 /dnas_title="PDIA2" /gene="PDIA2" /gene_synonym="PDA2" /gene_synonym="PDI" /gene_synonym="PDIR" /codon_start=1 /product="protein disulfide isomerase family A, member 2" /protein_id="AAI67826.1" /db_xref="GI:189442867" /db_xref="GeneID:64714" /db_xref="HGNC:14180" /db_xref="MIM:608012" /translation="MSRQLLPVLLLLLLRASCPWGQEQGARSPSEEPPEEEIPKEDGI LVLSRHTLGLALREHPALLVEFYAPWCGHCQALAPEYSKAAAVLAAESMVVTLAKVDG PAQRELAEEFGVTEYPTLKFFRNGNRTHPEEYTGPRDAEGIAEWLRRRVGPSAMRLED EAAAQALIGGRDLVVIGFFQDLQDEDVATFLALAQDALDMTFGLTDRPRLFQQFGLTK DTVVLFKKFDEGRADFPVDEELGLDLGDLSRFLVTHSMRLVTEFNSQTSAKIFAARIL NHLLLFVNQTLAAHRELLAGFGEAAPRFRGQVLFVVVDVAADNEHVLQYFGLKAEAAP TLRLVNLETTKKYAPVDGGPVTAASITAFCHAVLNGQVKPYLLSQEIPPDWDQRPVKT LVGKNFEQVAFDETKNVFVKFYAPWCTHCKEMAPAWEALAEKYQDHEDIIIAELDATA NELDAFAVHGFPTLKYFPAGPGRKVIEYKSTRDLETFSKFLDNGGVLPTEEPPEEPAA PFPEPPANSTMGSKEEL" misc_feature 3006..4568 /note="PDIA2 coding region" misc_feature 4569..4589 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4598) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4598) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4599..6784,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4598 /source="PDIA2-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgag ccgccagctt ctgcctgtac tgctgctgct gctgctcagg gcttcgtgcc 3061 catggggtca ggaacaggga gcgaggagcc cctcggagga gcctccagag gaggaaatcc 3121 ccaaggagga tgggatcttg gtgctgagcc gccacaccct gggcctggcc ctgcgggagc 3181 accctgccct gctggtggaa ttctatgccc cgtggtgtgg gcactgccag gccctggccc 3241 ccgagtacag caaggcagct gccgtgctcg cggccgagtc aatggtggtc acgctggcca 3301 aggtggatgg gcccgcgcag cgcgagctgg ctgaggagtt tggtgtgacg gagtacccta 3361 cgctcaagtt cttccgcaat gggaaccgca cgcacccgga ggagtacaca ggaccacggg 3421 acgctgaggg cattgccgag tggctgcgac ggcgggtggg gcccagtgcc atgcggctgg 3481 aggacgaggc ggccgcccag gcgctgatcg gtggccggga cctagtggtc attggcttct 3541 tccaggacct gcaggacgag gacgtggcca ccttcttggc cttggcccag gacgccctgg 3601 acatgacctt tggcctcaca gaccggccgc ggctctttca gcagtttggc ctcaccaagg 3661 acactgtggt tctcttcaag aagtttgatg aggggcgggc agacttcccc gtggacgagg 3721 agcttggcct ggacctgggg gatctgtcgc gcttcctggt cacacacagc atgcgcctgg 3781 tcacggagtt caacagccag acgtctgcca agatcttcgc ggccaggatc ctcaaccacc 3841 tgctgctgtt tgtcaaccag acgctggctg cgcaccggga gctcctagcg ggctttgggg 3901 aggcagctcc ccgcttccgg gggcaggtgc tgttcgtggt ggtggacgtg gcggccgaca 3961 atgagcacgt gctgcagtac tttggactca aggctgaggc agcccccact ctgcgcttgg 4021 tcaaccttga aaccactaag aagtatgcgc ctgtggatgg gggccctgtc accgcagcgt 4081 ccatcactgc tttctgccat gcagtcctca acggccaagt caagccctat ctcctgagcc 4141 aggagatacc ccctgattgg gatcagcggc cagttaagac cctcgtgggc aagaattttg 4201 agcaggtggc ttttgacgaa accaagaatg tgtttgtcaa gttctatgcc ccgtggtgca 4261 cccactgcaa ggagatggcc cctgcctggg aggcattggc tgagaagtac caagaccacg 4321 aggacatcat cattgctgag ctggatgcca cggccaacga gctggatgcc ttcgctgtgc 4381 acggcttccc tactctcaag tacttcccag cagggccagg tcggaaggtg attgaataca 4441 aaagcaccag ggacctggag actttctcca agttcctgga caacgggggc gtgctgccca 4501 cggaggagcc cccggaggag ccagcagccc cgttcccgga gccaccggcc aactccacta 4561 tggggtccga aaacttgtac tttcaaggct CCAGCTTTCT TGTACAAAGT GGTATGAGCA 4621 AGGGCGAGGA ACTGTTCACC GGCGTGGTGC CCATCCTGGT GGAACTGGAT GGCGACGTGA 4681 ACGGCCACAA GTTCTCTGTG CGGGGAGAGG GCGAAGGCGA CGCCACAAAT GGCAAGCTGA 4741 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC TTGGCCTACC CTCGTGACCA 4801 CACTGACCTA CGGCGTGCAG TGCTTCAGCA GATACCCCGA CCATATGAAG CGGCACGACT 4861 TCTTCAAGAG CGCCATGCCC GAGGGCTACG TGCAGGAACG GACCATCAGC TTCAAGGACG 4921 ACGGCACCTA CAAGACCAGA GCCGAAGTGA AGTTCGAGGG CGACACCCTC GTGAACCGGA 4981 TCGAGCTGAA GGGCATCGAT TTCAAAGAGG ACGGCAACAT CCTGGGCCAC AAGCTGGAGT 5041 ACAACTTCAA CAGCCACAAC GTGTACATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG 5101 CCAACTTCAA GATCCGGCAC AACGTGGAAG ATGGCAGCGT GCAGCTGGCC GACCACTACC 5161 AGCAGAACAC CCCCATCGGA GATGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA 5221 CCCAGAGCGT GCTGAGCAAG GACCCCAACG AGAAGCGGGA CCACATGGTG CTGCTGGAAT 5281 TCGTGACCGC CGCTGGCATC ACCCACGGCA TGAGCGGCCT GAACGACATC TTCGAGGCCC 5341 AGAAAATCGA GTGGCACGAG CACCACCATC ATCACCATCA CCACTGATGA TTCGAAAAAT 5401 AATAAGGATC CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA 5461 ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA 5521 TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT 5581 ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG 5641 CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT 5701 TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG 5761 GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC 5821 CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC 5881 CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC 5941 TTCCGCGTCT TCGAGATCTG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG 6001 CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA 6061 AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT 6121 GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGACTCGAG 6181 TTAAGGGCGA ATTGCCGATT AGGATCTTCC TAGAGCATGG CTACGTAGAT AAGTAGCATG 6241 GCGGGTTAAT CATTAACTAC AAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC 6301 GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC 6361 GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCCTTAATTA AGAGCTCATG GCGCGCCTAG 6421 GCCTTGACGG CCTTCCGCCA ATTCGCCCTA TAGTGAGTCG TATTACGTCG CGCTCACTGG 6481 CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG 6541 CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GAAACGCCCT 6601 TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGGAGCGCC CTGTAGCGGC CACTCAACCC 6661 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA 6721 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT 6781 TTAG //