LOCUS PDIA3-pGEc2-DEST 6724 bp DNA circular 26-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6202..6331) /note="3'-ITR" misc_feature complement(5900..6114) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5352..5893 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4531..4553 /note="attB2" misc_feature 5331..5336 /note="BstB1 site" misc_feature 4554..5249 /note="Super GFP" misc_feature 5250..5300 /note="AviTag" misc_feature 5301..5324 /note="His Tag" PCR_frame 3002..4531 /note="Reading Frame" /set="Set 1" source 3006..4508 /dnas_title="PDIA3-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:2159 IMAGE:3542405" /tissue_type="Lymph, Burkitt lymphoma" /clone_lib="NIH_MGC_8" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3006..4508 /dnas_title="PDIA3" /gene="PDIA3" /gene_synonym="ERp57" /gene_synonym="ERp60" /gene_synonym="ERp61" /gene_synonym="GRP57" /gene_synonym="HsT17083" /gene_synonym="P58" /gene_synonym="PI-PLC" /db_xref="GeneID:2923" /db_xref="HGNC:4606" /db_xref="MIM:602046" CDS 3006..4508 /dnas_title="protein disulfide isomerase family A, member 3" /gene="PDIA3" /gene_synonym="ERp57" /gene_synonym="ERp60" /gene_synonym="ERp61" /gene_synonym="GRP57" /gene_synonym="HsT17083" /gene_synonym="P58" /gene_synonym="PI-PLC" /codon_start=1 /product="protein disulfide isomerase family A, member 3" /protein_id="AAH14433.1" /db_xref="GI:15680173" /db_xref="GeneID:2923" /db_xref="HGNC:4606" /db_xref="MIM:602046" /translation="MRLRRLALFPGVALLLAAARLAAASDVLELTDDNFESRISDTGS AGLMLVEFFAPWCGHCKRLAPEYEAAATRLKGIVPLAKVDCTANTNTCNKYGVSGYPT LKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFF DDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDK TVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGS NYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGE KFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNN ENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGF PTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL" misc_feature 3006..4508 /note="PDIA3 coding region" misc_feature 4509..4529 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4538) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4538) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4539..6724,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4538 /source="PDIA3-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgcg cctccgccgc ctagcgctgt tcccgggtgt ggcgctgctt cttgccgcgg 3061 cccgcctcgc cgctgcctcc gacgtgctag aactcacgga cgacaacttc gagagtcgca 3121 tctccgacac gggctctgcg ggcctcatgc tcgtcgagtt cttcgccccc tggtgtggac 3181 actgcaagag acttgcacct gagtatgaag ctgcagctac cagattaaaa ggaatagtcc 3241 cattagcaaa ggttgattgc actgccaaca ctaacacctg taataaatat ggagtcagtg 3301 gatatccaac cctgaagata tttagagatg gtgaagaagc aggtgcttat gatggaccta 3361 ggactgctga tggaattgtc agccacttga agaagcaggc aggaccagct tcagtgcctc 3421 tcaggactga ggaagaattt aagaaattca ttagtgataa agatgcctct atagtaggtt 3481 ttttcgatga ttcattcagt gaggctcact ccgagttcct aaaagcagcc agcaacttga 3541 gggataacta ccgatttgca catacgaatg ttgagtctct ggtgaacgag tatgatgata 3601 atggagaggg tatcatctta tttcgtcctt cacatctcac taacaagttt gaggacaaga 3661 ctgtggcata tacagagcaa aaaatgacca gtggcaaaat taaaaagttt atccaggaaa 3721 acatttttgg tatctgccct cacatgacag aagacaataa agatttgata cagggcaagg 3781 acttacttat tgcttactat gatgtggact atgaaaagaa cgctaaaggt tccaactact 3841 ggagaaacag ggtaatgatg gtggcaaaga aattcctgga tgctgggcac aaactcaact 3901 ttgctgtagc tagccgcaaa acctttagcc atgaactttc tgattttggc ttggagagca 3961 ctgctggaga gattcctgtt gttgctatca gaactgctaa aggagagaag tttgtcatgc 4021 aggaggagtt ctcgcgtgat gggaaggctc tggagaggtt cctgcaggat tactttgatg 4081 gcaatctgaa gagatacctg aagtctgaac ctatcccaga gagcaatgat gggcctgtga 4141 aggtagtggt agcagagaat tttgatgaaa tagtgaataa tgaaaataaa gatgtgctga 4201 ttgaatttta tgccccttgg tgtggtcact gtaagaacct ggagcccaag tataaagaac 4261 ttggcgagaa gctcagcaaa gacccaaata tcgtcatagc caagatggat gccacagcca 4321 atgatgtgcc ttctccatat gaagtcagag gttttcctac catatacttc tctccagcca 4381 acaagaagct aaatccaaag aaatatgaag gtggccgtga attaagtgat tttattagct 4441 atctacaaag agaagctaca aacccccctg taattcaaga agaaaaaccc aagaagaaga 4501 agaaggcaga aaacttgtac tttcaaggct CCAGCTTTCT TGTACAAAGT GGTATGAGCA 4561 AGGGCGAGGA ACTGTTCACC GGCGTGGTGC CCATCCTGGT GGAACTGGAT GGCGACGTGA 4621 ACGGCCACAA GTTCTCTGTG CGGGGAGAGG GCGAAGGCGA CGCCACAAAT GGCAAGCTGA 4681 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC TTGGCCTACC CTCGTGACCA 4741 CACTGACCTA CGGCGTGCAG TGCTTCAGCA GATACCCCGA CCATATGAAG CGGCACGACT 4801 TCTTCAAGAG CGCCATGCCC GAGGGCTACG TGCAGGAACG GACCATCAGC TTCAAGGACG 4861 ACGGCACCTA CAAGACCAGA GCCGAAGTGA AGTTCGAGGG CGACACCCTC GTGAACCGGA 4921 TCGAGCTGAA GGGCATCGAT TTCAAAGAGG ACGGCAACAT CCTGGGCCAC AAGCTGGAGT 4981 ACAACTTCAA CAGCCACAAC GTGTACATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG 5041 CCAACTTCAA GATCCGGCAC AACGTGGAAG ATGGCAGCGT GCAGCTGGCC GACCACTACC 5101 AGCAGAACAC CCCCATCGGA GATGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA 5161 CCCAGAGCGT GCTGAGCAAG GACCCCAACG AGAAGCGGGA CCACATGGTG CTGCTGGAAT 5221 TCGTGACCGC CGCTGGCATC ACCCACGGCA TGAGCGGCCT GAACGACATC TTCGAGGCCC 5281 AGAAAATCGA GTGGCACGAG CACCACCATC ATCACCATCA CCACTGATGA TTCGAAAAAT 5341 AATAAGGATC CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA 5401 ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA 5461 TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT 5521 ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG 5581 CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT 5641 TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG 5701 GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC 5761 CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC 5821 CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC 5881 TTCCGCGTCT TCGAGATCTG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG 5941 CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA 6001 AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT 6061 GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGACTCGAG 6121 TTAAGGGCGA ATTGCCGATT AGGATCTTCC TAGAGCATGG CTACGTAGAT AAGTAGCATG 6181 GCGGGTTAAT CATTAACTAC AAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC 6241 GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC 6301 GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCCTTAATTA AGAGCTCATG GCGCGCCTAG 6361 GCCTTGACGG CCTTCCGCCA ATTCGCCCTA TAGTGAGTCG TATTACGTCG CGCTCACTGG 6421 CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG 6481 CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GAAACGCCCT 6541 TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGGAGCGCC CTGTAGCGGC CACTCAACCC 6601 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA 6661 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT 6721 TTAG //