LOCUS PDIA4-pGEc2-DEST 7144 bp DNA circular 26-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6622..6751) /note="3'-ITR" misc_feature complement(6320..6534) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5772..6313 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4951..4973 /note="attB2" misc_feature 5751..5756 /note="BstB1 site" misc_feature 4974..5669 /note="Super GFP" misc_feature 5670..5720 /note="AviTag" misc_feature 5721..5744 /note="His Tag" PCR_frame 3002..4951 /note="Reading Frame" /set="Set 1" source 3006..4928 /dnas_title="PDIA4-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:19707 IMAGE:3532913" /tissue_type="Muscle, rhabdomyosarcoma" /clone_lib="NIH_MGC_17" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3006..4928 /dnas_title="PDIA4" /gene="PDIA4" /gene_synonym="ERP70" /gene_synonym="ERP72" /db_xref="GeneID:9601" /db_xref="HGNC:30167" CDS 3006..4928 /dnas_title="PDIA4" /gene="PDIA4" /gene_synonym="ERP70" /gene_synonym="ERP72" /codon_start=1 /product="protein disulfide isomerase family A, member 4" /protein_id="AAH11754.1" /db_xref="GI:15079928" /db_xref="GeneID:9601" /db_xref="HGNC:30167" /translation="MRPRKAFLLLLLLGLVQLLAVAGAEGPDEDSSNRENAIEDEEEE EEEDDDEEEDDLEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYE KIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAVDYEGSRTQEEI VAKVREVSQPDWTPPPEVTLVLTKENFDEVVNDADIILVEFYAPWCGHCKKLAPEYEK AAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFRKGRPYDYNGPREKYGIV DYMIEQSGPPSKEILTLKQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDY KFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKY ALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTF AIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKG KLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYN SLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDL EHLSKFIEEHATKLSRTKEEL" misc_feature 3006..4928 /note="PDIA4 coding region" misc_difference 4661..4661 /dnas_title="PDIA4" /gene="PDIA4" /gene_synonym="ERP70" /gene_synonym="ERP72" /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 4929..4949 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4958) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4958) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4959..7144,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4958 /source="PDIA4-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgag gccccggaaa gccttcctgc tcctgctgct cttggggctg gtgcagctgc 3061 tggccgtggc gggtgccgag ggcccggacg aggattcttc taacagagaa aatgccattg 3121 aggatgaaga ggaggaggag gaggaagatg atgatgagga agaagacgac ttggaagtta 3181 aggaagaaaa tggagtcttg gtcctaaatg atgcaaactt tgataatttt gtggctgaca 3241 aagacacagt gctgctggag ttttatgctc catggtgtgg acattgcaag cagtttgctc 3301 cggaatatga aaaaattgcc aacatattaa aggataaaga tcctcccatt cctgttgcca 3361 agatcgatgc aacctcagcg tctgtgctgg ccagcaggtt tgatgtgagt ggctacccca 3421 ccatcaagat ccttaagaag gggcaggctg tagactacga gggctccaga acccaggaag 3481 aaattgttgc caaggtcaga gaagtctccc agcccgactg gacgcctcca ccagaagtca 3541 cgcttgtgtt gaccaaagag aactttgatg aagttgtgaa tgatgcagat atcattctgg 3601 tggagtttta tgccccatgg tgtggacact gcaagaaact tgcccccgag tatgagaagg 3661 ccgccaagga gctcagcaag cgttctcctc caattcccct ggcaaaggtc gacgccaccg 3721 cagaaacaga cctggccaag aggtttgatg tctctggcta tcccaccctg aaaattttcc 3781 gcaaaggaag gccttatgac tacaacggcc cacgagaaaa atatggaatc gttgattaca 3841 tgatcgagca gtccgggcct ccctccaagg agattctgac cctgaagcag gtccaggagt 3901 tcctgaagga tggagacgat gtcatcatca tcggggtctt taagggggag agtgacccag 3961 cctaccagca ataccaggat gccgctaaca acctgagaga agattacaaa tttcaccaca 4021 ctttcagcac agaaatagca aagttcttga aagtctccca ggggcagttg gttgtaatgc 4081 agcctgagaa attccagtcc aagtatgagc cccggagcca catgatggac gtccagggct 4141 ccacccagga ctcggccatc aaggacttcg tgctgaagta cgccctgccc ctggttggcc 4201 accgcaaggt gtcaaacgat gctaagcgct acaccaggcg ccccctggtg gtcgtctact 4261 acagtgtgga cttcagcttt gattacagag ctgcaactca gttttggcgg agcaaagtcc 4321 tagaggtggc caaggacttc cctgagtaca cctttgccat tgcggacgaa gaggactatg 4381 ctggggaggt gaaggacctg gggctcagcg agagtgggga ggatgtcaat gccgccatcc 4441 tggacgagag tgggaagaag ttcgccatgg agccagagga gtttgactct gacaccctcc 4501 gcgagtttgt cactgctttc aaaaaaggaa aactgaagcc agtcatcaaa tcccagccag 4561 tgcccaagaa caacaaggga cccgtcaagg tcgtggtggg aaagaccttt gactccattg 4621 tgatggaccc caagaaggac gtcctcatcg agttctacgc accatggtgc gggcactgca 4681 agcagctaga gcccgtgtac aacagcctgg ccaagaagta caagggccaa aagggcctgg 4741 tcatcgccaa gatggacgcc actgccaacg acgtccccag cgaccgctat aaggtggagg 4801 gcttccccac catctacttc gcccccagtg gggacaaaaa gaacccagtt aaatttgagg 4861 gtggagacag agatctggag catttgagca agtttataga agaacatgcc acaaaactga 4921 gcaggaccga aaacttgtac tttcaaggct CCAGCTTTCT TGTACAAAGT GGTATGAGCA 4981 AGGGCGAGGA ACTGTTCACC GGCGTGGTGC CCATCCTGGT GGAACTGGAT GGCGACGTGA 5041 ACGGCCACAA GTTCTCTGTG CGGGGAGAGG GCGAAGGCGA CGCCACAAAT GGCAAGCTGA 5101 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC TTGGCCTACC CTCGTGACCA 5161 CACTGACCTA CGGCGTGCAG TGCTTCAGCA GATACCCCGA CCATATGAAG CGGCACGACT 5221 TCTTCAAGAG CGCCATGCCC GAGGGCTACG TGCAGGAACG GACCATCAGC TTCAAGGACG 5281 ACGGCACCTA CAAGACCAGA GCCGAAGTGA AGTTCGAGGG CGACACCCTC GTGAACCGGA 5341 TCGAGCTGAA GGGCATCGAT TTCAAAGAGG ACGGCAACAT CCTGGGCCAC AAGCTGGAGT 5401 ACAACTTCAA CAGCCACAAC GTGTACATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG 5461 CCAACTTCAA GATCCGGCAC AACGTGGAAG ATGGCAGCGT GCAGCTGGCC GACCACTACC 5521 AGCAGAACAC CCCCATCGGA GATGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA 5581 CCCAGAGCGT GCTGAGCAAG GACCCCAACG AGAAGCGGGA CCACATGGTG CTGCTGGAAT 5641 TCGTGACCGC CGCTGGCATC ACCCACGGCA TGAGCGGCCT GAACGACATC TTCGAGGCCC 5701 AGAAAATCGA GTGGCACGAG CACCACCATC ATCACCATCA CCACTGATGA TTCGAAAAAT 5761 AATAAGGATC CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA 5821 ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA 5881 TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT 5941 ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG 6001 CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT 6061 TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG 6121 GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC 6181 CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC 6241 CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC 6301 TTCCGCGTCT TCGAGATCTG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG 6361 CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA 6421 AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT 6481 GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGACTCGAG 6541 TTAAGGGCGA ATTGCCGATT AGGATCTTCC TAGAGCATGG CTACGTAGAT AAGTAGCATG 6601 GCGGGTTAAT CATTAACTAC AAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC 6661 GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC 6721 GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCCTTAATTA AGAGCTCATG GCGCGCCTAG 6781 GCCTTGACGG CCTTCCGCCA ATTCGCCCTA TAGTGAGTCG TATTACGTCG CGCTCACTGG 6841 CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG 6901 CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GAAACGCCCT 6961 TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGGAGCGCC CTGTAGCGGC CACTCAACCC 7021 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA 7081 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT 7141 TTAG //