LOCUS GNPTAB-pGEn2 8805 bp DNA circular 20-SEP-2016 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 7433..7974 /note="WPRE" misc_recomb 7395..7417 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(7981..8195) /note="BGH pA" misc_feature complement(8283..8412) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" misc_feature 3868..3890 /note="TEV site" misc_feature 3891..7388 /note="OPTIMIZED GNPTAB coding region" vector join(7403..8805,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..7402 /source="GNPTAB pDONR" /type="Entry Clone" source 1..8805 /dnas_title="GNPTAB-pGEn2" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc TGGTCCAGGG 3901 ACCAGTACCA CGTGCTGTTC GACAGCTACA GAGACAATAT CGCCGGCAAG AGCTTCCAGA 3961 ACAGACTGTG CCTGCCCATG CCCATCGACG TGGTGTATAC CTGGGTCAAC GGCACCGACC 4021 TGGAACTGCT GAAAGAACTG CAGCAGGTCC GAGAGCAGAT GGAAGAGGAA CAGAAAGCCA 4081 TGAGAGAGAT CCTGGGCAAG AACACCACCG AGCCCACCAA GAAGTCTGAG AAGCAGCTGG 4141 AATGCCTGCT GACCCACTGC ATCAAGGTGC CAATGCTGGT GCTGGATCCT GCCCTGCCTG 4201 CCAACATCAC ACTGAAGGAT CTGCCTAGCC TGTACCCCAG CTTTCACAGC GCCAGCGACA 4261 TCTTCAACGT GGCCAAGCCC AAGAACCCCT CCACCAATGT GTCCGTGGTC GTGTTCGATA 4321 GCACCAAGGA CGTGGAAGAT GCCCACTCTG GGCTGCTGAA GGGCAATAGC AGACAGACAG 4381 TGTGGCGGGG CTACCTGACC ACAGACAAAG AAGTGCCTGG CCTGGTCCTG ATGCAGGATC 4441 TGGCCTTTCT GAGCGGCTTC CCACCCACCT TCAAAGAGAC AAACCAGCTG AAAACAAAGC 4501 TGCCCGAGAA TCTGAGCAGC AAAGTGAAGC TGCTCCAGCT GTACTCTGAG GCCTCTGTGG 4561 CCCTGCTCAA GCTGAACAAC CCCAAGGACT TCCAGGAACT GAACAAGCAG ACCAAGAAAA 4621 ACATGACAAT CGATGGCAAA GAGCTGACCA TCTCCCCCGC CTATCTGCTG TGGGATCTGA 4681 GCGCCATCAG CCAGAGCAAG CAGGATGAGG ATATCAGCGC CTCCAGATTC GAGGACAACG 4741 AGGAACTGCG GTACAGCCTG AGATCCATCG AAAGACATGC CCCCTGGGTC CGAAATATCT 4801 TTATCGTGAC CAACGGCCAG ATTCCCAGCT GGCTGAACCT GGACAACCCT CGCGTGACCA 4861 TCGTGACACA CCAGGATGTG TTCAGAAACC TGAGCCATCT GCCCACCTTT AGCTCCCCAG 4921 CCATCGAGAG CCACATCCAC AGAATCGAGG GCCTGTCCCA GAAGTTCATC TACCTGAACG 4981 ACGACGTGAT GTTCGGAAAA GACGTGTGGC CCGACGACTT CTACAGCCAC TCTAAGGGCC 5041 AGAAGGTGTA CCTGACTTGG CCCGTGCCTA ATTGCGCCGA GGGATGTCCT GGCTCTTGGA 5101 TCAAGGACGG CTACTGCGAC AAGGCCTGCA ACAATAGCGC CTGTGATTGG GATGGCGGCG 5161 ATTGCAGTGG CAATTCCGGC GGCTCTAGAT ATATTGCTGG CGGCGGAGGC ACAGGCTCTA 5221 TCGGAGTGGG ACAGCCTTGG CAGTTTGGAG GCGGCATCAA TAGCGTGTCC TACTGCAACC 5281 AGGGCTGCGC CAATTCTTGG CTGGCCGACA AGTTCTGCGA TCAGGCCTGT AATGTGCTGA 5341 GCTGTGGATT CGACGCTGGC GATTGTGGCC AGGATCACTT CCACGAGCTG TACAAGGTCA 5401 TCCTCCTGCC CAACCAGACA CACTACATCA TCCCTAAGGG CGAGTGTCTG CCCTACTTCA 5461 GCTTTGCCGA AGTGGCCAAA AGAGGCGTGG AAGGCGCCTA CAGCGATAAC CCTATCATCA 5521 GACACGCCTC CATTGCCAAC AAGTGGAAAA CCATCCACCT GATCATGCAC AGCGGCATGA 5581 ACGCCACCAC AATCCACTTC AACCTGACCT TTCAGAATAC CAACGACGAG GAATTCAAGA 5641 TGCAGATCAC CGTCGAGGTG GACACCAGAG AGGGCCCTAA ACTGAATTCT ACCGCCCAGA 5701 AGGGCTACGA AAACCTGGTG TCTCCTATCA CCCTGCTGCC TGAGGCCGAG ATTCTGTTCG 5761 AGGATATTCC CAAAGAGAAG CGGTTCCCCA AGTTCAAGCG GCACGATGTG AATTCCACCA 5821 GACGGGCCCA GGAAGAAGTC AAGATCCCCC TGGTCAACAT CTCTCTGCTG CCCAAGGATG 5881 CCCAGCTGAG CCTGAATACT CTGGATCTGC AGCTCGAGCA CGGCGACATT ACCCTGAAGG 5941 GATACAACCT GTCCAAGTCC GCCCTGCTGA GAAGTTTCCT GATGAACAGC CAGCACGCCA 6001 AGATCAAGAA TCAGGCCATC ATCACCGACG AGACTAACGA CTCACTGGTG GCCCCCCAGG 6061 AAAAACAGGT CCACAAGAGC ATCCTGCCTA ACAGCCTGGG CGTGTCCGAA AGGCTGCAGA 6121 GACTGACATT TCCCGCCGTC AGCGTGAAAG TGAATGGCCA CGATCAGGGA CAGAACCCCC 6181 CTCTCGATCT GGAAACCACC GCCAGATTTC GGGTGGAAAC CCACACCCAG AAAACAATCG 6241 GCGGCAATGT GACCAAAGAA AAGCCCCCCT CCCTGATTGT GCCCCTGGAA AGCCAGATGA 6301 CAAAAGAGAA AAAGATTACC GGCAAGGAAA AAGAAAACTC CAGAATGGAA GAAAACGCCG 6361 AGAACCACAT TGGCGTGACA GAGGTGCTGC TGGGCAGAAA GCTGCAGCAC TACACAGACT 6421 CCTACCTGGG CTTCCTGCCT TGGGAGAAGA AGAAGTACTT TCAGGACCTC CTGGACGAAG 6481 AAGAGTCCCT CAAGACACAG CTGGCCTACT TTACCGACAG CAAGAACCGG GCCAGATACA 6541 AGAGGGACAC CTTCGCCGAT TCCCTGAGAT ACGTGAACAA GATCCTGAAC AGCAAGTTCG 6601 GCTTCACCAG CAGAAAGGTG CCCGCCCACA TGCCTCACAT GATCGACAGG ATCGTCATGC 6661 AGGAACTCCA GGATATGTTT CCCGAAGAGT TCGATAAGAC CAGCTTCCAC AAAGTGCGGC 6721 ACAGCGAGGA CATGCAGTTC GCCTTCAGCT ACTTCTACTA CCTGATGAGC GCCGTGCAGC 6781 CCCTGAATAT CTCCCAGGTG TTCGACGAAG TGGACACAGA CCAGAGCGGA GTGCTGTCCG 6841 ATCGGGAAAT TAGAACACTG GCCACCCGGA TCCACGAACT GCCACTGAGT CTGCAGGACC 6901 TGACCGGACT GGAACACATG CTGATCAACT GCTCCAAGAT GCTGCCCGCT GACATCACCC 6961 AGCTCAACAA TATCCCCCCC ACCCAGGAAT CCTACTACGA CCCTAATCTG CCCCCAGTGA 7021 CCAAGTCCCT CGTGACAAAT TGCAAGCCCG TGACCGATAA GATCCATAAG GCCTACAAGG 7081 ACAAGAACAA GTACCGCTTC GAGATCATGG GCGAGGAAGA GATCGCCTTT AAGATGATCC 7141 GGACCAACGT GTCCCATGTC GTGGGCCAGC TCGACGACAT CCGGAAGAAC CCTAGAAAGT 7201 TCGTGTGCCT GAATGACAAC ATCGATCACA ACCACAAAGA CGCCCAGACC GTGAAGGCCG 7261 TGCTGAGAGA TTTCTACGAG AGCATGTTCC CCATCCCTAG CCAGTTTGAG CTGCCTAGAG 7321 AGTACCGGAA CAGATTCCTG CACATGCATG AACTGCAGGA GTGGCGGGCC TATCGGGACA 7381 AACTGAAGTA GGACCCAGCT TTCTTGTACA AAGTGGTTGA TAGCTTGGAT CCAATCAACC 7441 TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC 7501 GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT 7561 CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT 7621 TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG 7681 CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC 7741 GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC 7801 TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT 7861 TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC 7921 GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGAGATCT 7981 GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC 8041 TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG 8101 CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG 8161 GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGACTCGA GTTAAGGGCG AATTGCCGAT 8221 TAGGATCTTC CTAGAGCATG GCTACGTAGA TAAGTAGCAT GGCGGGTTAA TCATTAACTA 8281 CAAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA 8341 GGCCGGGCGA CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA 8401 GCGAGCGCGC AGCCTTAATT AAGAGCTCAT GGCGCGCCTA GGCCTTGACG GCCTTCCGCC 8461 AATTCGCCCT ATAGTGAGTC GTATTACGTC GCGCTCACTG GCCGTCGTTT TACAACGTCG 8521 TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC 8581 CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGAAACGCCC TTCCCAACAG TTGCGCAGCC 8641 TGAATGGCGA ATGGGAGCGC CCTGTAGCGG CCACTCAACC CTATCTCGGT CTATTCTTTT 8701 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 8761 AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAG //