LOCUS SULF1-pGEn2 7854 bp DNA circular 29-SEP-2016 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 6482..7023 /note="WPRE" misc_recomb 6444..6466 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(7030..7244) /note="BGH pA" misc_feature complement(7332..7461) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" CDS 3868..6443 /label=SULF1_Nterm misc_feature 3870..3890 /note="TEV site" misc_feature 3891..6440 /note="OPTIMIZED SULF1 coding region" vector join(6452..7854,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..6451 /source="SULF1-pDONR" /type="Entry Clone" source 1..7854 /dnas_title="SULF1-pGEn2" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTCTG AGAACCTGTA TTTTCAAGGC AGCACCGTGC 3901 GGAGCCCCCG GTTTAGAGGA AGAATCCAGC AGGAACGGAA GAACATCCGG CCCAACATCA 3961 TCCTGGTGCT GACCGACGAT CAGGACGTGG AACTGGGAAG CCTGCAGGTC ATGAACAAGA 4021 CCCGGAAGAT CATGGAACAC GGCGGAGCCA CCTTCATCAA CGCCTTTGTG ACCACCCCCA 4081 TGTGCTGCCC TAGCAGATCT AGCATGCTGA CAGGCAAATA CGTGCACAAC CACAACGTGT 4141 ACACCAACAA CGAGAACTGC AGCAGCCCTA GCTGGCAGGC CATGCACGAA CCTAGAACCT 4201 TCGCCGTGTA CCTGAACAAC ACCGGCTACA GAACCGCCTT CTTCGGCAAG TATCTGAACG 4261 AGTACAACGG CAGCTACATC CCCCCTGGAT GGCGAGAATG GCTGGGCCTG ATCAAGAACA 4321 GCCGGTTCTA CAACTACACC GTGTGCCGGA ACGGCATCAA AGAGAAGCAC GGCTTCGACT 4381 ACGCCAAGGA CTACTTCACC GACCTGATCA CCAACGAGAG CATCAATTAC TTCAAGATGT 4441 CCAAGCGGAT GTACCCCCAC CGGCCCGTGA TGATGGTCAT TTCTCATGCC GCTCCTCACG 4501 GCCCAGAAGA TAGCGCCCCT CAGTTTAGCA AGCTGTACCC CAATGCCAGC CAGCACATCA 4561 CCCCCAGCTA CAATTACGCC CCCAACATGG ACAAGCACTG GATCATGCAG TACACAGGCC 4621 CCATGCTGCC CATCCACATG GAATTCACCA ACATCCTGCA GCGGAAGCGG CTGCAGACCC 4681 TGATGTCTGT GGACGACAGC GTGGAACGGC TGTACAACAT GCTGGTGGAA ACCGGCGAGC 4741 TGGAAAACAC CTACATCATC TACACCGCCG ACCACGGCTA CCACATCGGA CAGTTTGGAC 4801 TGGTCAAGGG CAAGAGCATG CCCTACGACT TCGATATCCG GGTGCCATTC TTCATCAGAG 4861 GCCCTTCCGT GGAACCTGGC TCCATCGTGC CTCAGATCGT GCTGAATATC GACCTGGCCC 4921 CCACCATCCT GGATATCGCT GGACTGGATA CCCCTCCTGA TGTGGACGGC AAGTCTGTGC 4981 TGAAGCTGCT GGACCCTGAG AAGCCCGGCA ACAGATTCAG GACCAACAAG AAGGCCAAGA 5041 TCTGGCGGGA CACCTTCCTG GTCGAGAGAG GCAAGTTCCT GCGGAAGAAA GAGGAAAGCT 5101 CCAAGAACAT TCAGCAGAGC AACCATCTGC CTAAGTACGA GCGCGTGAAA GAGCTGTGCC 5161 AGCAGGCCAG ATACCAGACC GCTTGTGAAC AGCCTGGCCA GAAATGGCAG TGCATCGAGG 5221 ATACCAGCGG CAAGCTGCGG ATCCACAAGT GCAAGGGACC TAGCGACCTG CTGACTGTGC 5281 GGCAGAGCAC CAGAAATCTG TACGCCAGAG GCTTCCACGA CAAGGACAAA GAGTGCTCCT 5341 GCAGAGAGAG CGGCTATAGA GCCAGCAGAA GCCAGAGAAA GTCCCAGAGA CAGTTCCTGA 5401 GAAACCAGGG CACCCCCAAG TACAAGCCCA GATTCGTGCA CACCAGACAG ACCAGAAGCC 5461 TGAGCGTCGA GTTCGAGGGC GAGATCTACG ACATCAACCT GGAGGAGGAA GAGGAACTCC 5521 AGGTCCTCCA GCCTCGGAAT ATCGCCAAGA GACACGATGA GGGACACAAG GGCCCAAGAG 5581 ATCTGCAGGC CTCTTCTGGC GGCAATAGAG GCAGAATGCT GGCCGATAGC TCTAACGCCG 5641 TGGGCCCTCC TACAACCGTC AGAGTGACCC ACAAATGCTT CATCCTGCCC AATGACAGCA 5701 TCCACTGCGA GCGGGAACTG TACCAGTCTG CCAGAGCTTG GAAGGACCAC AAGGCCTACA 5761 TCGACAAAGA AATCGAGGCC CTGCAGGACA AGATTAAGAA CCTGAGAGAA GTGCGGGGCC 5821 ACCTGAAGCG GAGAAAGCCT GAAGAGTGCA GCTGCTCCAA GCAGAGCTAC TACAACAAGG 5881 AAAAGGGCGT CAAGAAGCAG GAAAAGCTGA AAAGCCATCT GCACCCCTTC AAAGAGGCCG 5941 CCCAGGAAGT CGATTCTAAG CTCCAGCTGT TTAAAGAGAA CAACCGCAGA CGCAAGAAAG 6001 AACGCAAAGA AAAGCGCCGG CAGCGCAAGG GCGAAGAATG TAGTCTGCCT GGCCTGACCT 6061 GCTTCACCCA CGATAACAAT CATTGGCAGA CCGCCCCCTT CTGGAACCTC GGAAGCTTTT 6121 GTGCCTGCAC CAGCAGCAAC AACAATACCT ACTGGTGCCT GCGGACCGTG AACGAGACAC 6181 ACAACTTTCT GTTCTGCGAG TTCGCCACCG GCTTTCTGGA ATACTTCGAC ATGAACACCG 6241 ACCCCTACCA GCTGACCAAC ACAGTGCACA CAGTGGAAAG AGGCATCCTG AACCAGCTGC 6301 ATGTGCAGCT CATGGAGCTG AGATCCTGCC AGGGCTACAA GCAGTGCAAC CCCAGACCTA 6361 AGAATCTGGA CGTGGGAAAC AAGGACGGCG GCTCCTATGA TCTGCATAGA GGCCAGCTGT 6421 GGGACGGATG GGAAGGATAA GACCCAGCTT TCTTGTACAA AGTGGTTGAT AGCTTGGATC 6481 CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC 6541 TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG 6601 TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT 6661 GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC 6721 TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC 6781 TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT 6841 GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT 6901 CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT 6961 CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT 7021 TCGAGATCTG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC 7081 GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA 7141 ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC 7201 AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGACTCGAG TTAAGGGCGA 7261 ATTGCCGATT AGGATCTTCC TAGAGCATGG CTACGTAGAT AAGTAGCATG GCGGGTTAAT 7321 CATTAACTAC AAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC 7381 GCTCACTGAG GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC 7441 AGTGAGCGAG CGAGCGCGCA GCCTTAATTA AGAGCTCATG GCGCGCCTAG GCCTTGACGG 7501 CCTTCCGCCA ATTCGCCCTA TAGTGAGTCG TATTACGTCG CGCTCACTGG CCGTCGTTTT 7561 ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC 7621 CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GAAACGCCCT TCCCAACAGT 7681 TGCGCAGCCT GAATGGCGAA TGGGAGCGCC CTGTAGCGGC CACTCAACCC TATCTCGGTC 7741 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 7801 ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTAG //