LOCUS GAL3ST1-pGEn2-DE 6468 bp DNA circular 27-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 5096..5637 /note="WPRE" misc_recomb 5058..5080 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(5644..5858) /note="BGH pA" misc_feature complement(5946..6075) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" misc_feature 3868..3890 /note="TEV site" source 3891..5051 /dnas_title="GAL3ST1-pGEn2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:29648 IMAGE:4562507" /tissue_type="Kidney, renal cell adenocarcinoma" /clone_lib="NIH_MGC_14" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3891..5051 /dnas_title="GAL3ST1" /gene="GAL3ST1" /gene_synonym="CST" /db_xref="GeneID:9514" /db_xref="HGNC:24240" /db_xref="MIM:602300" CDS 3891..5051 /dnas_title="galactose-3-O-sulfotransferase 1" /gene="GAL3ST1" /gene_synonym="CST" /codon_start=1 /product="galactose-3-O-sulfotransferase 1" /protein_id="AAH19077.1" /db_xref="GI:17512187" /db_xref="GeneID:9514" /db_xref="HGNC:24240" /db_xref="MIM:602300" /translation="MLPPQKKPWESMAKGLVLGALFTSFLLLMYSYAVPPLHAGLAST TPEAAASCSPPALEPEAVIRANGSAGECQPRRNIVFLKTHKTASSTLLNILFRFGQKH RLKFAFPNGRNDFDYPTFFARSLVQDYRPGACFNIICNHMRFHYDEVRGLVPTNAIFI TVLRDPARLFESSFHYFGPVVPLTWKLSAGDKLTEFLQDPDRYYDPNGFNAHYLRNLL FFDLGYDNSLDPSSPQVQEHILEVERRFHLVLLQEYFDESLVLLKDLLCWELEDVLYF KLNARRDSPVPRLSGELYGRATAWNMLDSHLYRHFNASFWRKVEAFGRERMAREVAAL RHANERMRTICIDGGHAVDAAAIQDEAMQPWQPLGTKSILGYNLKKSIGQRHAQLCRR MLTPEIQYLMDLGANLWVTKLWKFIRDFLRW" misc_feature 3891..5051 /note="GAL3ST1 coding region" vector join(5066..6468,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..5065 /source="GAL3ST1-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc catgccggcc 3901 tggcctccac gaccccggag gccgcagcgt cctgctctcc acctgcactc gagccagagg 3961 cagtgatccg ggccaacggc tcggcggggg agtgccagcc gcggcgcaac atcgtgttct 4021 tgaagacgca caagacggcc agcagcaccc tgctcaacat cctgttccgc ttcggccaga 4081 agcaccggct caagttcgcc ttccctaacg gccgcaatga cttcgactac ccgaccttct 4141 tcgcccgcag cctggtgcag gactatcggc ccggggcctg cttcaacatc atctgcaacc 4201 acatgcgctt ccactacgac gaggtgcgcg gcctggtgcc gaccaacgcc atcttcatca 4261 cggtgctccg cgaccccgcc cgcttgttcg agtcctcctt ccactacttc gggccggtgg 4321 tgcccctcac gtggaagctc tcggccggcg acaagctgac cgagttcctg caagacccgg 4381 atcgctacta cgaccccaac ggcttcaatg cccactacct ccgaaacctg ctcttcttcg 4441 acctgggcta tgacaacagc ctggacccca gcagcccgca ggtgcaggag cacatcctgg 4501 aggtggagcg tcgcttccac ctggtgctcc ttcaagagta cttcgacgag tcgctggtgc 4561 tgctgaagga cctgctgtgc tgggagctgg aggacgtgct ctacttcaag ctcaacgccc 4621 gccgcgactc gcccgtgccg cggctctcgg gggagctgta tgggcgcgcc accgcctgga 4681 acatgctgga ctcccacctc taccgccact tcaacgccag cttctggcgc aaggtggagg 4741 ccttcgggcg ggagcgcatg gcccgcgagg tggccgccct gcgccatgcc aacgagcgca 4801 tgcggaccat ctgcatcgac gggggccacg ccgtggacgc cgccgccatc caggacgagg 4861 ccatgcagcc ctggcagccg ctgggcacca agtccatcct gggctacaac ctcaagaaga 4921 gcatcgggca gcggcacgcg cagctctgcc ggcgcatgct cacgcccgag atccagtacc 4981 tgatggacct cggcgccaac ctgtgggtca ccaagctctg gaagttcatt cgcgatttcc 5041 tgcggtggtg aTAGGACCCA GCTTTCTTGT ACAAAGTGGT TGATAGCTTG GATCCAATCA 5101 ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT 5161 TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC 5221 TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC 5281 CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG 5341 GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC 5401 CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG 5461 CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG 5521 TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC 5581 AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGAGA 5641 TCTGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT 5701 TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA 5761 TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG 5821 GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGACT CGAGTTAAGG GCGAATTGCC 5881 GATTAGGATC TTCCTAGAGC ATGGCTACGT AGATAAGTAG CATGGCGGGT TAATCATTAA 5941 CTACAAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGCTC GCTCGCTCAC 6001 TGAGGCCGGG CGACCAAAGG TCGCCCGACG CCCGGGCTTT GCCCGGGCGG CCTCAGTGAG 6061 CGAGCGAGCG CGCAGCCTTA ATTAAGAGCT CATGGCGCGC CTAGGCCTTG ACGGCCTTCC 6121 GCCAATTCGC CCTATAGTGA GTCGTATTAC GTCGCGCTCA CTGGCCGTCG TTTTACAACG 6181 TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT 6241 CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGAAACG CCCTTCCCAA CAGTTGCGCA 6301 GCCTGAATGG CGAATGGGAG CGCCCTGTAG CGGCCACTCA ACCCTATCTC GGTCTATTCT 6361 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 6421 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTAG //