LOCUS HS2ST2-pGEn1-DES 5560 bp DNA circular 29-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4188..4729 /note="WPRE" misc_recomb 4150..4172 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(4736..4950) /note="BGH pA" misc_feature complement(5038..5167) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4143 /dnas_title="HS2ST2-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /chromosome="16" /map="16p12" /tissue_type="brain" /note="isolated by hybridization screening using IMAGE Consortium clone c-20d10 found in GenBank Accession Number F07258" gene 3166..4143 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" CDS 3166..4143 /dnas_title="heparan sulfate D-glucosaminyl 3-O-sulfotransferase-2" /gene="3OST2" /allele="3OST2-1" /EC_number="2.8.2.23" /note="3-OST-2" /codon_start=1 /product="heparan sulfate D-glucosaminyl 3-O-sulfotransferase-2" /protein_id="AAD30206.1" /db_xref="GI:4835719" /translation="MAYRVLGRAGPPQPRRARRLLFAFTLSLSCTYLCYSFLCCCDDL GRSRLLGAPRCLRGPSAGGQKLLQKSRPCDPSGPTPSEPSAPSAPAAAVPAPRLSGSN HSGSPKLGTKRLPQALIVGVKKGGTRAVLEFIRVHPDVRALGTEPHFFDRNYGRGLDW YRSLMPRTLESQITLEKTPSYFVTQEAPRRIFNMSRDTKLIVVVRNPVTRAISDYTQT LSKKPDIPTFEGLSFRNRTLGLVDVSWNAIRIGMYVLHLESWLQYFPLAQIHFVSGER LITDPAGEMGRVQDFLGIKRFITDKHFYFNKTKGFPCLKKTESSLLPRCLGKSKGRTH VQIDPEVIDQLREFYRPYNIKFYETVGQDFRWE" misc_feature 3166..3369 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="SPLAG domain; Ser, Pro, Leu, Ala, Gly enriched; other site" misc_feature 3205..3261 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative cystine bridged peptide loop; other site" misc_feature 3277..3279 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative O-glycosylation site" misc_feature 3283..3285 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative O-glycosylation site" misc_feature 3292..3294 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative O-glycosylation site" misc_feature 3301..3303 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative O-glycosylation site" misc_feature 3337..3345 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative N-glycosylation site" misc_feature 3370..4143 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="sulfotransferase domain; other site" misc_feature 3394..3432 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative beta-strand/P-loop/alpha-helix motif; binds 5'-phosphate of 3'-phosphoadenosine 5'-phosphate, PAPS; binding site" misc_feature 3166..4143 /note="HS3ST2 coding region" misc_feature 3619..3627 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative N-glycosylation site" misc_feature 3637..3684 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative sheet-loop-helix structure for binding the 3'-phosphate of PAPS" misc_feature 3700..3702 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative O-glycosylation site" misc_feature 3745..3753 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative N-glycosylation site" misc_feature 3793..3822 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative structure for binding 5'-sulfate of PAPS" misc_feature 3958..3966 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative N-glycosylation site" variation 3774..3774 /dnas_title="3OST2" /gene="3OST2" /note="3OST2-2 allele; silent wobble position mutation" /replace="a" misc_feature 3979..4017 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative cystine bridged peptide loop; other site" misc_feature 4072..4080 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative N-glycosylation site" vector join(4158..5560,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4157 /source="HS3ST2-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcgacga cctgggtcgg 3181 agccgcctcc tcggcgcgcc tcgctgcctc cgcggcccca gcgcgggcgg ccagaaactt 3241 ctccagaagt cccgcccctg tgatccctcc gggccgacgc ccagcgagcc cagcgctccc 3301 agcgcgcccg ccgccgccgt gcccgcccct cgcctctccg gttccaacca ctccggctca 3361 cccaagctgg gtaccaagcg gttgccccaa gccctcattg tgggcgtgaa gaaggggggc 3421 acccgggccg tgctggagtt tatccgagta cacccggacg tgcgggcctt gggcacggaa 3481 ccccacttct ttgacaggaa ctacggccgc gggctggatt ggtacaggag cctgatgccc 3541 aggaccctcg agagccagat cacgctggag aagacgccca gctactttgt cactcaagag 3601 gctcctcgac gcatcttcaa catgtcccga gacaccaagc tgatcgtggt tgtgcggaac 3661 cctgtgaccc gtgccatctc tgattacacg cagacactct ccaagaagcc cgacatcccg 3721 acctttgagg gcctctcctt ccgcaaccgc accctgggcc tggtggacgt gtcgtggaac 3781 gccatccgca tcggcatgta cgtgctgcac ctggagagct ggctgcagta cttcccgcta 3841 gctcagattc acttcgtcag tggcgagcga ctcatcactg acccggccgg cgagatgggg 3901 cgagtccagg acttcctggg cattaagaga ttcatcacgg acaagcactt ctatttcaac 3961 aagaccaaag gattcccttg cttgaaaaaa acagaatcga gcctcctgcc tcgatgcttg 4021 ggcaaatcaa aagggagaac tcatgtacag attgatcctg aagtgataga ccagctccga 4081 gaattttata gaccgtataa tatcaaattt tatgaaaccg ttgggcagga cttcaggtgg 4141 gaaTAGGACC CAGCTTTCTT GTACAAAGTG GTTGATAGCT TGGATCCAAT CAACCTCTGG 4201 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT 4261 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT 4321 TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA 4381 GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG 4441 CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG 4501 AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA 4561 ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA 4621 CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC 4681 TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGA GATCTGCCTC 4741 GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC 4801 CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG 4861 TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA 4921 TTGGGAAGAC AATAGCAGGC ATGCTGGGGA CTCGAGTTAA GGGCGAATTG CCGATTAGGA 4981 TCTTCCTAGA GCATGGCTAC GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAAGG 5041 AACCCCTAGT GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG 5101 GGCGACCAAA GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG 5161 CGCGCAGCCT TAATTAAGAG CTCATGGCGC GCCTAGGCCT TGACGGCCTT CCGCCAATTC 5221 GCCCTATAGT GAGTCGTATT ACGTCGCGCT CACTGGCCGT CGTTTTACAA CGTCGTGACT 5281 GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT 5341 GGCGTAATAG CGAAGAGGCC CGCACCGAAA CGCCCTTCCC AACAGTTGCG CAGCCTGAAT 5401 GGCGAATGGG AGCGCCCTGT AGCGGCCACT CAACCCTATC TCGGTCTATT CTTTTGATTT 5461 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT 5521 TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG //