LOCUS HS3ST2-pGEn2-DES 6285 bp DNA circular 29-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4913..5454 /note="WPRE" misc_recomb 4875..4897 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(5461..5675) /note="BGH pA" misc_feature complement(5763..5892) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" misc_feature 3868..3890 /note="TEV site" source 3891..4868 /dnas_title="HS3ST2-pGEn2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /chromosome="16" /map="16p12" /tissue_type="brain" /note="isolated by hybridization screening using IMAGE Consortium clone c-20d10 found in GenBank Accession Number F07258" gene 3891..4868 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" CDS 3891..4868 /dnas_title="heparan sulfate D-glucosaminyl 3-O-sulfotransferase-2" /gene="3OST2" /allele="3OST2-1" /EC_number="2.8.2.23" /note="3-OST-2" /codon_start=1 /product="heparan sulfate D-glucosaminyl 3-O-sulfotransferase-2" /protein_id="AAD30206.1" /db_xref="GI:4835719" /translation="MAYRVLGRAGPPQPRRARRLLFAFTLSLSCTYLCYSFLCCCDDL GRSRLLGAPRCLRGPSAGGQKLLQKSRPCDPSGPTPSEPSAPSAPAAAVPAPRLSGSN HSGSPKLGTKRLPQALIVGVKKGGTRAVLEFIRVHPDVRALGTEPHFFDRNYGRGLDW YRSLMPRTLESQITLEKTPSYFVTQEAPRRIFNMSRDTKLIVVVRNPVTRAISDYTQT LSKKPDIPTFEGLSFRNRTLGLVDVSWNAIRIGMYVLHLESWLQYFPLAQIHFVSGER LITDPAGEMGRVQDFLGIKRFITDKHFYFNKTKGFPCLKKTESSLLPRCLGKSKGRTH VQIDPEVIDQLREFYRPYNIKFYETVGQDFRWE" misc_feature 3891..4094 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="SPLAG domain; Ser, Pro, Leu, Ala, Gly enriched; other site" misc_feature 3930..3986 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative cystine bridged peptide loop; other site" misc_feature 4002..4004 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative O-glycosylation site" misc_feature 4008..4010 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative O-glycosylation site" misc_feature 4017..4019 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative O-glycosylation site" misc_feature 4026..4028 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative O-glycosylation site" misc_feature 4062..4070 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative N-glycosylation site" misc_feature 4095..4868 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="sulfotransferase domain; other site" misc_feature 4119..4157 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative beta-strand/P-loop/alpha-helix motif; binds 5'-phosphate of 3'-phosphoadenosine 5'-phosphate, PAPS; binding site" misc_feature 3891..4868 /note="HS3ST2 coding region" misc_feature 4344..4352 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative N-glycosylation site" misc_feature 4362..4409 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative sheet-loop-helix structure for binding the 3'-phosphate of PAPS" misc_feature 4425..4427 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative O-glycosylation site" misc_feature 4470..4478 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative N-glycosylation site" misc_feature 4518..4547 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative structure for binding 5'-sulfate of PAPS" misc_feature 4683..4691 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative N-glycosylation site" variation 4499..4499 /dnas_title="3OST2" /gene="3OST2" /note="3OST2-2 allele; silent wobble position mutation" /replace="a" misc_feature 4704..4742 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative cystine bridged peptide loop; other site" misc_feature 4797..4805 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative N-glycosylation site" vector join(4883..6285,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..4882 /source="HS3ST2-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc gacgacctgg 3901 gtcggagccg cctcctcggc gcgcctcgct gcctccgcgg ccccagcgcg ggcggccaga 3961 aacttctcca gaagtcccgc ccctgtgatc cctccgggcc gacgcccagc gagcccagcg 4021 ctcccagcgc gcccgccgcc gccgtgcccg cccctcgcct ctccggttcc aaccactccg 4081 gctcacccaa gctgggtacc aagcggttgc cccaagccct cattgtgggc gtgaagaagg 4141 ggggcacccg ggccgtgctg gagtttatcc gagtacaccc ggacgtgcgg gccttgggca 4201 cggaacccca cttctttgac aggaactacg gccgcgggct ggattggtac aggagcctga 4261 tgcccaggac cctcgagagc cagatcacgc tggagaagac gcccagctac tttgtcactc 4321 aagaggctcc tcgacgcatc ttcaacatgt cccgagacac caagctgatc gtggttgtgc 4381 ggaaccctgt gacccgtgcc atctctgatt acacgcagac actctccaag aagcccgaca 4441 tcccgacctt tgagggcctc tccttccgca accgcaccct gggcctggtg gacgtgtcgt 4501 ggaacgccat ccgcatcggc atgtacgtgc tgcacctgga gagctggctg cagtacttcc 4561 cgctagctca gattcacttc gtcagtggcg agcgactcat cactgacccg gccggcgaga 4621 tggggcgagt ccaggacttc ctgggcatta agagattcat cacggacaag cacttctatt 4681 tcaacaagac caaaggattc ccttgcttga aaaaaacaga atcgagcctc ctgcctcgat 4741 gcttgggcaa atcaaaaggg agaactcatg tacagattga tcctgaagtg atagaccagc 4801 tccgagaatt ttatagaccg tataatatca aattttatga aaccgttggg caggacttca 4861 ggtgggaaTA GGACCCAGCT TTCTTGTACA AAGTGGTTGA TAGCTTGGAT CCAATCAACC 4921 TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC 4981 GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT 5041 CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT 5101 TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG 5161 CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC 5221 GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC 5281 TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT 5341 TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC 5401 GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGAGATCT 5461 GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC 5521 TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG 5581 CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG 5641 GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGACTCGA GTTAAGGGCG AATTGCCGAT 5701 TAGGATCTTC CTAGAGCATG GCTACGTAGA TAAGTAGCAT GGCGGGTTAA TCATTAACTA 5761 CAAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA 5821 GGCCGGGCGA CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA 5881 GCGAGCGCGC AGCCTTAATT AAGAGCTCAT GGCGCGCCTA GGCCTTGACG GCCTTCCGCC 5941 AATTCGCCCT ATAGTGAGTC GTATTACGTC GCGCTCACTG GCCGTCGTTT TACAACGTCG 6001 TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC 6061 CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGAAACGCCC TTCCCAACAG TTGCGCAGCC 6121 TGAATGGCGA ATGGGAGCGC CCTGTAGCGG CCACTCAACC CTATCTCGGT CTATTCTTTT 6181 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 6241 AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAG //