LOCUS NDST1-pGEn2 7836 bp DNA circular 29-SEP-2016 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 6464..7005 /note="WPRE" misc_recomb 6426..6448 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(7012..7226) /note="BGH pA" misc_feature complement(7314..7443) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" CDS 3868..6425 /label=NDST1 misc_feature 3870..3890 /note="TEV site" misc_feature 3891..6422 /note="OPTIMIZED NDST1 coding region" vector join(6434..7836,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..6433 /source="NDST1-pDONR" /type="Entry Clone" source 1..7836 /dnas_title="NDST1-pGEn2" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTCTG AGAACCTGTA TTTTCAAGGC GGCTGGAAGC 3901 GGGGCCTGGA ACCTTCTGCT GATGCCCCCG AACCCGACTG CGGAGATCCT CCACCTGTGG 3961 CCCCTAGCAG ACTGCTGCCT CTGAAACCAG TGCAGGCCGC CACCCCAAGC AGAACCGATC 4021 CTCTGGTGCT GGTGTTCGTG GAAAGCCTGT ACAGCCAGCT GGGCCAGGAA GTGGTGGCCA 4081 TCCTGGAAAG CAGCCGGTTC AAGTACCGGA CCGAGATCGC CCCTGGCAAG GGCGATATGC 4141 CCACCCTGAC CGATAAGGGC AGAGGCAGAT TCGCCCTGAT CATCTACGAG AACATCCTGA 4201 AATACGTGAA CCTGGACGCC TGGAACAGAG AGCTGCTGGA CAAGTACTGC GTGGCCTACG 4261 GCGTGGGCAT CATCGGATTC TTCAAGGCCA ACGAGAACTC CCTGCTGAGC GCCCAGCTGA 4321 AGGGCTTCCC ACTGTTCCTG CACAGCAACC TGGGCCTGAA GGACTGCAGC ATCAACCCCA 4381 AGAGCCCCCT GCTGTACGTG ACCAGACCCA GCGAGGTGGA AAAGGGCGTG CTGCCTGGCG 4441 AGGACTGGAC CGTGTTCCAG AGCAACCACA GCACCTACGA GCCCGTGCTG CTGGCCAAGA 4501 CCAGAAGCAG CGAGAGCATC CCTCACCTGG GAGCCGATGC CGGACTGCAT GCTGCCCTGC 4561 ATGCTACCGT GGTGCAGGAT CTGGGCCTGC ACGACGGCAT CCAGAGAGTG CTGTTCGGCA 4621 ACAACCTGAA CTTCTGGCTG CACAAGCTGG TGTTTGTGGA CGCCGTGGCC TTCCTGACCG 4681 GCAAGAGACT GAGCCTGCCC CTGGACCGGT ACATCCTGGT GGACATCGAC GACATCTTCG 4741 TGGGCAAAGA GGGCACCCGG ATGAAGGTGG AAGATGTGAA GGCCCTGTTC GACACCCAGA 4801 ACGAGCTGAG AGCCCACATC CCCAACTTCA CCTTCAATCT GGGCTACAGC GGCAAGTTCT 4861 TCCACACCGG CACCAACGCC GAAGATGCCG GCGACGATCT GCTGCTGAGC TACGTGAAAG 4921 AATTCTGGTG GTTCCCTCAC ATGTGGTCCC ACATGCAGCC CCACCTGTTC CACAACCAGA 4981 GCGTGCTGGC CGAGCAGATG GCCCTGAACA AGAAATTCGC CGTGGAACAT GGCATCCCCA 5041 CCGACATGGG ATATGCCGTG GCCCCTCACC ACAGCGGCGT GTACCCTGTG CATGTGCAGC 5101 TGTACGAGGC CTGGAAACAA GTGTGGTCCA TCAGAGTGAC CAGCACCGAA GAGTACCCCC 5161 ACCTGAAGCC CGCCAGATAC AGACGGGGCT TCATCCACAA CGGCATCATG GTGCTGCCCC 5221 GGCAGACCTG CGGCCTGTTT ACCCACACCA TCTTCTACAA CGAGTACCCT GGCGGCAGCA 5281 GCGAGCTGGA TAAGATCATC AACGGCGGCG AGCTGTTTCT GACAGTGCTG CTGAACCCCA 5341 TCAGCATCTT CATGACCCAC CTGAGCAACT ACGGCAACGA CCGGCTGGGC CTGTACACCT 5401 TCAAACACCT CGTGCGGTTT CTGCACAGCT GGACCAACCT GCGGCTGCAG ACACTGCCTC 5461 CAGTGCAGCT GGCCCAGAAG TACTTTCAGA TCTTCAGCGA AGAGAAGGAC CCCCTGTGGC 5521 AGGACCCCTG CGAGGACAAG CGGCACAAGG ACATCTGGTC CAAAGAGAAA ACCTGCGACC 5581 GGTTCCCCAA GCTGCTGATC ATCGGCCCCC AGAAAACCGG CACAACCGCC CTGTACCTGT 5641 TCCTGGGCAT GCACCCCGAC CTGAGCAGCA ACTACCCCAG CAGCGAAACC TTCGAGGAAA 5701 TCCAGTTCTT CAACGGGCAC AACTACCACA AGGGCATCGA CTGGTACATG GAATTCTTCC 5761 CAATCCCCAG CAACACCACC AGCGACTTCT ACTTCGAGAA GTCCGCCAAC TACTTCGACT 5821 CCGAGGTGGC CCCAAGAAGG GCCGCTGCTC TGCTGCCTAA GGCCAAGGTG CTGACCATCC 5881 TGATCAACCC CGCCGACAGA GCCTACAGCT GGTATCAGCA TCAGCGGGCC CACGACGATC 5941 CCGTGGCTCT GAAGTACACC TTTCACGAAG TGATCACCGC CGGCAGCGAC GCCAGCTCCA 6001 AACTGAGAGC ACTGCAGAAC CGGTGCCTGG TGCCTGGGTG GTACGCCACC CACATCGAGA 6061 GATGGCTGTC CGCCTACCAC GCCAATCAGA TCCTGGTGCT GGACGGCAAG CTGCTGCGGA 6121 CCGAACCCGC CAAAGTGATG GATATGGTGC AGAAATTCCT GGGCGTGACC AACACCATCG 6181 ATTACCACAA GACCCTGGCC TTCGACCCCA AGAAAGGCTT CTGGTGTCAG CTGCTGGAAG 6241 GCGGCAAGAC CAAGTGCCTG GGCAAGAGCA AGGGCCGGAA GTACCCCGAG ATGGACCTGG 6301 ACAGCAGGGC CTTTCTGAAG GATTACTACC GGGACCACAA CATCGAGCTG TCTAAGCTGC 6361 TGTATAAGAT GGGCCAGACC CTGCCCACCT GGCTGAGAGA GGACCTGCAG AACACCAGAT 6421 AGGACCCAGC TTTCTTGTAC AAAGTGGTTG ATAGCTTGGA TCCAATCAAC CTCTGGATTA 6481 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG 6541 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC 6601 CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA 6661 ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC 6721 CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT 6781 CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC 6841 CGTGGTGTTG TCGGGGAAGC TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG 6901 GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC 6961 TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGAGATC TGCCTCGACT 7021 GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG 7081 GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG 7141 AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG 7201 GAAGACAATA GCAGGCATGC TGGGGACTCG AGTTAAGGGC GAATTGCCGA TTAGGATCTT 7261 CCTAGAGCAT GGCTACGTAG ATAAGTAGCA TGGCGGGTTA ATCATTAACT ACAAGGAACC 7321 CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTCGC TCGCTCACTG AGGCCGGGCG 7381 ACCAAAGGTC GCCCGACGCC CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG 7441 CAGCCTTAAT TAAGAGCTCA TGGCGCGCCT AGGCCTTGAC GGCCTTCCGC CAATTCGCCC 7501 TATAGTGAGT CGTATTACGT CGCGCTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA 7561 AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG 7621 TAATAGCGAA GAGGCCCGCA CCGAAACGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG 7681 AATGGGAGCG CCCTGTAGCG GCCACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 7741 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 7801 GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAG //