LOCUS NDST3-pGEn2 7824 bp DNA circular 21-SEP-2016 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 6452..6993 /note="WPRE" misc_recomb 6414..6436 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(7000..7214) /note="BGH pA" misc_feature complement(7302..7431) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" misc_feature 3868..3890 /note="TEV site" misc_feature 3891..6410 /note="OPTIMIZED NDST3 coding region" vector join(6422..7824,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..6421 /source="NDST3 PDONR221 " /type="Entry Clone" source 1..7824 /dnas_title="NDST3-pGEn2" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc AGCGGCTACA 3901 AGCAAGAGAA CGAGCTGAGC GAGACAGCCT CTGAGGTTGA CTGTGGCGAT CTGCAGCATC 3961 TGCCCTACCA GCTGATGGAA GTGAAGGCCA TGAAGCTGTT CGACGCCAGC AGAACCGATC 4021 CTACCGTGCT GGTGTTCGTG GAAAGCCAGT ATAGCAGCCT CGGCCAGGAC ATCATCATGA 4081 TCCTGGAAAG CAGCCGGTTC CAGTACCACA TCGAGATTGC CCCTGGAAAG GGCGACCTGC 4141 CTGTGCTGAT CGACAAGATG AAGGGCAAGT ACATCCTGAT CATCTACGAG AACATCCTGA 4201 AGTACATCAA CATGGACAGC TGGAACAGAA GCCTGCTGGA CAAGTACTGC GTGGAATACG 4261 GCGTGGGCGT GATCGGCTTT CACAAGACCT CTGAGAAGTC CGTGCAGAGC TTCCAGCTGA 4321 AGGGCTTCCC ATTCAGCATC TACGGCAACC TGGCCGTGAA GGACTGCTGT ATCAATCCTC 4381 ACAGCCCACT GATCAGAGTG ACCAAGAGCA GCAAGCTGGA AAAGGGCAGC CTGCCAGGCA 4441 CCGATTGGAC CGTGTTTCAG ATCAACCACA GCGCCTACCA GCCTGTGATC TTCGCCAAAG 4501 TGAAAACCCC TGAGAATCTG AGCCCCAGCA TCAGCAAGGG CGCCTTCTAT GCCACCATCA 4561 TCCACGATCT GGGCCTGCAC GATGGCATCC AGAGAGTGCT GTTCGGCAAC AACCTGAACT 4621 TCTGGCTGCA CAAGCTGATC TTTATCGACG CCATCAGCTT TCTGAGCGGC AAGAGACTGA 4681 CACTGAGCCT GGACCGCTAC ATCCTGGTGG ACATCGACGA CATCTTCGTG GGCAAAGAGG 4741 GCACCAGAAT GAACACCAAC GACGTGAAGG CCCTGCTGGA TACCCAGAAT CTGCTGAGAG 4801 CCCAGATCAC CAACTTCACC TTCAACCTGG GCTTCAGCGG CAAGTTCTAC CACACCGGCA 4861 CCGAAGAGGA AGATGAGGGC GACGATTGTC TGCTGGGCAG CGTGGACGAG TTCTGGTGGT 4921 TTCCTCACAT GTGGTCCCAC ATGCAGCCCC ACCTGTTCCA CAATGAGAGC AGCCTGGTGG 4981 AACAGATGAT CCTGAACAAG AAGTTCGCCC TGGAACACGG CATCCCCACC GATATGGGAT 5041 ATGCCGTGGC TCCTCACCAC TCTGGCGTGT ACCCTGTGCA TGTGCAGCTG TACGAGGCCT 5101 GGAAGAAAGT GTGGAACATC AAGATCACCA GCACCGAGGA ATACCCTCAC CTGAAGCCAG 5161 CCAGATACAG ACGGGGCTTC ATCCACAAGA ACATCATGGT GCTGCCTCGG CAGACCTGCG 5221 GCCTGTTTAC CCACACCATC TTCTACAAAG AGTACCCTGG CGGCCCTAAA GAGCTGGATA 5281 AGTCTATCCA AGGCGGCGAG CTGTTCTTCA CCGTGGTGCT GAACCCCATC TCCATCTTCA 5341 TGACCCACCT GAGCAATTAC GGCAACGACA GACTGGGCCT GTACACCTTC GTGAACCTGG 5401 CCAACTTCGT GAAGTCCTGG ACCAATCTGA GACTGCAGAC CCTGCCTCCA GTGCAGCTGG 5461 CCCACAAGTA CTTCGAGCTG TTTCCCGACC AGAAAGACCC TCTGTGGCAG AACCCCTGCG 5521 ACGACAAGAG ACACCGGGAC ATCTGGTCCA AAGAAAAGAC CTGCGACCGG CTGCCTAAGT 5581 TCCTGGTCAT CGGACCTCAG AAAACCGGCA CAACAGCCCT GTACCTGTTC CTCGTGATGC 5641 ACCCCTCTAT CCTGAGCAAC AGCCCATCTC CTAAGACCTT CGAGGAAGTG CAGTTCTTCA 5701 ACCGGAACAA CTACCACCGG GGCATCGACT GGTACATGGA TTTCTTCCCC GTGCCTTCCA 5761 ACGTGACCAC CGACTTTCTG TTCGAGAAGT CTGCCAACTA CTTCCACAGC GAGGAAGCCC 5821 CTAAAAGGGC CGCCTCTCTG GTGCCTAAGG CCAAGATCAT CACAATCCTG ATCGACCCCA 5881 GCGACCGGGC CTACTCTTGG TATCAGCATC AGAGAAGCCA CGAGGACCCT GCCGCTCTGA 5941 AGTTCAGCTT CTACGAAGTG ATCAGCGCCG GACCTAGAGC ACCTTCTGAA CTGAGGGCCC 6001 TGCAGAAAAG ATGCCTGGTG CCTGGATGGT ACGCCTCTCA CATTGAGCGG TGGCTGGTGT 6061 ACTTCCCACC ATTCCAGCTG CTGATCATCG ACGGACAGCA GCTGAGAACT GACCCCGCCA 6121 CAGTGATGGA CGAGGTGCAG AAGTTTCTGG GCGTCCTGCC TCACTACAAC TACAGCGAGG 6181 CCCTGACCTT CGACAGCCAC AAAGGCTTTT GGTGCCAGCT GCTCGAAGAG GGCAAGACCA 6241 AGTGCCTGGG CAAGAGCAAG GGCAGAAAGT ACCCACCTAT GGACAGCGAC AGCCGGACCT 6301 TCCTGAGCAG CTACTACAGG GACCACAACG TGGAACTGAG CAAGCTGCTG CACAAACTGG 6361 GCCAGCCTCT GCCTTCTTGG CTGAGACAAG AGCTGCAGAA AGTCCGCTAG GACCCAGCTT 6421 TCTTGTACAA AGTGGTTGAT AGCTTGGATC CAATCAACCT CTGGATTACA AAATTTGTGA 6481 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT 6541 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA 6601 ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT 6661 GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT 6721 CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG 6781 CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC 6841 GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG 6901 GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT 6961 GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGAGATCTG CCTCGACTGT GCCTTCTAGT 7021 TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT 7081 CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT 7141 TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC 7201 AGGCATGCTG GGGACTCGAG TTAAGGGCGA ATTGCCGATT AGGATCTTCC TAGAGCATGG 7261 CTACGTAGAT AAGTAGCATG GCGGGTTAAT CATTAACTAC AAGGAACCCC TAGTGATGGA 7321 GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC 7381 CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCCTTAATTA 7441 AGAGCTCATG GCGCGCCTAG GCCTTGACGG CCTTCCGCCA ATTCGCCCTA TAGTGAGTCG 7501 TATTACGTCG CGCTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT 7561 TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA 7621 GGCCCGCACC GAAACGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGGAGCGCC 7681 CTGTAGCGGC CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 7741 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC 7801 AAAATATTAA CGCTTACAAT TTAG //