LOCUS NDST4-pGEn2 7821 bp DNA circular 21-SEP-2016 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 6449..6990 /note="WPRE" misc_recomb 6411..6433 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(6997..7211) /note="BGH pA" misc_feature complement(7299..7428) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" misc_feature 3868..3890 /note="TEV site" misc_feature 3891..6407 /note="OPTIMIZED NDST4 coding region" vector join(6419..7821,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..6418 /source="NDST4 PDONR221" /type="Entry Clone" source 1..7821 /dnas_title="NDST4-pGEn2" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc AGCGGCTACA 3901 AGCAAGAGAT GACCCTGATC GAGACAACCG CCGAGGCCGA GTGCACCGAT ATCAAGATCC 3961 TGCCTTACCG GTCCATGGAA CTGAAAACCG TGAAGCCCAT CGACACCAGC AAGACAGATC 4021 CCACCGTGCT GCTGTTCGTG GAAAGCCAGT ATAGCCAGCT CGGCCAGGAC ATCATTGCCA 4081 TCCTGGAAAG CAGCCGGTTC CAGTACCACA TGGTTATCGC CCCTGGAAAG GGCGACATCC 4141 CTCCTCTGAC CGATAACGGC AAGGGCAAGT ACACCCTGGT CATCTACGAG AACATCCTGA 4201 AATACGTCAG CATGGACAGC TGGAACAGAG AGCTGCTGGA AAAGTACTGC GTGGAATACT 4261 CCGTGTCCAT CATCGGCTTC CACAAGGCCA ACGAGAACTC CCTGCCTAGC ACACAGCTGA 4321 AGGGCTTCCC TCTGAACCTG TTCAACAATC TGGCCCTGAA GGACTGCTTC GTGAACCCTC 4381 AGTCTCCCCT GCTGCACATC ACAAAGGCCC CTAAGGTGGA AAAGGGACCC CTGCCTGGCG 4441 AGGATTGGAC CATCTTCCAG TACAACCACA GCACCTACCA GCCTGTGCTG CTGACCGAGC 4501 TGCAGACCGA GAAATCTCTG AGCAGCCTGT CTAGCAAGAC CCTGTTCGCC ACCGTGATCC 4561 AGGATCTGGG ACTGCACGAT GGCATCCAGA GAGTGCTGTT CGGCAACAAC CTGAACTTCT 4621 GGCTGCACAA GCTGATCTTT ATCGACGCCA TCAGCTTTCT GAGCGGCAAG AGGCTGACAC 4681 TGAGCCTGGA CAGATATATC CTGGTGGACA TCGACGACAT CTTCGTGGGC AAAGAGGGCA 4741 CCAGAATGAA CGTGAAGGAT GTGAAGGCCC TGCTCGAAAC CCAGAACCTG CTGAGAACCC 4801 AGGTGGCCAA CTTCACCTTC AATCTGGGCT TCAGCGGCAA GTTCTACCAC ACCGGCACCG 4861 AAGAGGAAGA TGAGGGCGAC GATCTGCTGC TGAGATCCGT GGACGAGTTC TGGTGGTTCC 4921 CTCACATGTG GTCCCACATG CAGCCCCACC TGTTTCACAA TGAGTCCAGC CTGGTGGAAC 4981 AGATGATCCT GAACAAAGAG TTCGCCCTGG AACACGGCAT CCCCATCAAT ATGGGATACG 5041 CCGTGGCTCC TCACCACTCT GGCGTGTACC CTGTGCACAT TCAGCTGTAC GCCGCCTGGA 5101 AGAAAGTGTG GGGCATCCAA GTGACCAGCA CCGAGGAATA CCCTCACCTG AAGCCTGCCA 5161 GATACAGAAA GGGCTTCATC CACAACAGCA TCATGGTGCT GCCTAGACAG ACCTGCGGCC 5221 TGTTCACCCA CACCATCTTC TACAAAGAGT ACCCTGGCGG CCCTCAAGAG CTGGACAAGT 5281 CTATTAGAGG CGGCGAGCTG TTCCTGACCA TCCTGCTGAA CCCCATCAGC ATCTTCATGA 5341 CCCACCTGAG CAACTACGGC AACGACAGGC TGGGCCTGTA CACCTTCGTG AATCTGGTCA 5401 ACTTTGTGCA GTCCTGGACC AACCTGAAAC TGCAGACACT GCCTCCAGTG CAGCTGGCCC 5461 ACCAGTACTT CGAACTGTTC CCCGAGCAGA AAGACCCTCT GTGGCAGAAC CCCTGCGACG 5521 ACAAGAGACA CAAGGACATC TGGTCCCGGG AAAAGACCTG CGACCATCTG CCTAAGTTCC 5581 TCGTGATCGG CCCTCAGAAA ACCGGCACAA CAGCCCTGTA CCTGTTCCTG CTGATGCACC 5641 CCAGCATCAT CAGCAACCTG CCTTCTCCAA AGACCTTCGA GGAAGTGCAG TTCTTCAACG 5701 GGAACAACTA CCACAAGGGC ATCGACTGGT ACATGGATTT CTTCCCCACA CCGTCCAACA 5761 CCACCAGCGA CTTTCTGTTC GAGAAGTCCG CCAACTACTT CCACAGCGAG GAAGCCCCTA 5821 GAAGGGCTGC CTCTCTGGTG CCTAAGGCCA AGATCATCAC CATCCTGATC GACCCCAGCG 5881 ACCGGGCCTA TTCTTGGTAT CAGCATCAGA GAAGCCACGA GGACCCTGCC GCTCTGCGGT 5941 TCAACTTCTA CGAAGTGATC TCCACAGGCC ACTGGGCCCC TAGCGATCTG AAAACCCTGC 6001 AGAGAAGATG CCTGGTGCCT GGATGGTACG CCGTGCACAT TGAGAGATGG CTGACCTACT 6061 TTGCCACCAG CCAGCTGCTG ATCATCGACG GCCAGCAGCT GAGATCTGAC CCCGCCACAG 6121 TGATGGACGA GGTGCAGAAA TTCCTGGGCG TGACCCCTCG GTACAATTAC AGCGAGGCCC 6181 TGACATTCGA TCCCCAGAAA GGCTTCTGGT GTCAGCTGCT CGAAGGCGGC AAGACCAAGT 6241 GCCTGGGCAA GAGCAAGGGC AGAAAGTACC CACCTATGGA CCCCGAGAGC CGGACCTTCC 6301 TGAGCAATTA CTACCGGGAC CACAACGTGG AACTGAGCAA ACTGCTGCAT AGACTGGGCC 6361 AGCCTCTGCC TTCTTGGCTG CGGCAAGAAC TCCAGAAAGT CCGCTAGGAC CCAGCTTTCT 6421 TGTACAAAGT GGTTGATAGC TTGGATCCAA TCAACCTCTG GATTACAAAA TTTGTGAAAG 6481 ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT 6541 GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC 6601 CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG 6661 CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT 6721 TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT 6781 TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG 6841 GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC 6901 GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT 6961 GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG AGATCTGCCT CGACTGTGCC TTCTAGTTGC 7021 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC 7081 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT 7141 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG 7201 CATGCTGGGG ACTCGAGTTA AGGGCGAATT GCCGATTAGG ATCTTCCTAG AGCATGGCTA 7261 CGTAGATAAG TAGCATGGCG GGTTAATCAT TAACTACAAG GAACCCCTAG TGATGGAGTT 7321 GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA AGGTCGCCCG 7381 ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCC TTAATTAAGA 7441 GCTCATGGCG CGCCTAGGCC TTGACGGCCT TCCGCCAATT CGCCCTATAG TGAGTCGTAT 7501 TACGTCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC 7561 CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC 7621 CCGCACCGAA ACGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG GAGCGCCCTG 7681 TAGCGGCCAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 7741 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA 7801 ATATTAACGC TTACAATTTA G //