LOCUS NAGPA-pGEc2 6771 bp DNA circular 21-SEP-2016 FEATURES Location/Qualifiers misc_feature complement(6249..6378) /note="3'-ITR" misc_feature complement(5947..6161) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5399..5940 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4578..4600 /note="attB2" misc_feature 5378..5383 /note="BstB1 site" misc_feature 4601..5296 /note="Super GFP" misc_feature 5297..5347 /note="AviTag" misc_feature 5348..5371 /note="His Tag" PCR_frame 3011..4577 /note="Reading Frame" /set="Set 1" misc_feature 3011..4555 /note="OPTIMIZED NAGPA coding region" misc_feature 4556..4577 /note="TEV" vector join(4586..6771,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4585 /source="NAGPA pDONR" /type="Entry Clone" source 1..6771 /dnas_title="NAGPA-pGEc2" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCGCCACC ATGGCCACAT CTACTGGAAG ATGGCTGCTG CTGAGACTGG CCCTGTTTGG 3061 ATTTCTGTGG GAAGCCTCTG GCGGACTGGA TAGCGGAGCC AGCAGAGATG ATGACCTGCT 3121 GCTCCCTTAT CCTCGGGCCA GAGCTAGACT GCCCAGAGAT TGCACAAGAG TGCGGGCTGG 3181 CAATAGAGAG CACGAGTCTT GGCCTCCACC TCCTGCTACA CCTGGTGCTG GTGGACTGGC 3241 TGTGCGGACC TTTGTGTCCC ACTTCAGAGA TAGAGCCGTG GCCGGACATC TGACAAGAGC 3301 TGTGGAACCC CTGAGAACCT TCAGCGTGCT GGAACCAGGT GGACCTGGTG GATGTGCTGC 3361 TAGACGCAGA GCCACAGTGG AAGAAACAGC CAGAGCCGCC GATTGCAGAG TGGCCCAGAA 3421 TGGCGGCTTC TTCAGAATGA ATAGCGGCGA GTGCCTGGGC AACGTGGTGT CCGATGAGAG 3481 AAGAGTGTCC TCTAGTGGCG GCCTGCAGAA TGCCCAGTTT GGCATCCGAA GAGATGGCAC 3541 CCTGGTCACC GGCTACCTGT CTGAGGAAGA GGTGCTGGAC ACCGAGAACC CTTTTGTGCA 3601 GCTGCTGTCT GGCGTCGTGT GGCTGATCAG AAACGGCAGC ATCTACATCA ACGAGAGCCA 3661 GGCCACCGAG TGCGACGAAA CCCAGGAAAC AGGCAGCTTC AGCAAGTTCG TGAACGTGAT 3721 CAGCGCCAGA ACCGCCATCG GCCACGATAG AAAAGGCCAG CTGGTGCTCT TTCACGCCGA 3781 TGGACAGACA GAGCAGAGGG GCATTAACCT GTGGGAGATG GCCGAGTTCC TGCTGAAACA 3841 GGACGTGGTC AACGCCATCA ATCTGGATGG CGGCGGAAGC GCCACATTCG TGCTGAATGG 3901 AACACTGGCC AGCTACCCCA GCGACCACTG CCAGGATAAT ATGTGGCGGT GCCCTAGACA 3961 GGTGTCCACA GTCGTGTGTG TGCACGAGCC TAGATGCCAG CCTCCTGATT GTCACGGACA 4021 CGGCACATGT GTGGATGGCC ACTGTCAGTG CACCGGCCAC TTTTGGAGAG GCCCTGGATG 4081 TGATGAGCTG GACTGTGGCC CTAGCAATTG CAGCCAGCAC GGCCTGTGTA CCGAGACAGG 4141 ATGCAGATGT GACGCCGGCT GGACAGGCTC TAACTGCAGC GAAGAATGTC CCCTGGGATG 4201 GCATGGACCA GGTTGTCAGA GGCCTTGCAA GTGCGAGCAC CACTGTCCCT GTGACCCTAA 4261 GACAGGCAAC TGCTCCGTGT CCAGAGTGAA GCAGTGTCTG CAGCCTCCAG AAGCCACACT 4321 GAGAGCCGGC GAGCTGAGCT TCTTTACCAG AACAGCTTGG CTGGCTCTGA CCCTGGCACT 4381 GGCTTTTCTG CTCCTCATCT CTACAGCCGC CAACCTGTCT CTCCTGCTGT CAAGAGCCGA 4441 GCGGAATAGA AGGCTGCACG GCGATTATGC CTACCACCCC CTGCAGGAAA TGAACGGCGA 4501 ACCTCTGGCC GCCGAAAAAG AACAGCCTGG GGGAGCCCAC AACCCCTTCA AAGATgaaaa 4561 cttgtacttt caaggctCCA GCTTTCTTGT ACAAAGTGGT ATGAGCAAGG GCGAGGAACT 4621 GTTCACCGGC GTGGTGCCCA TCCTGGTGGA ACTGGATGGC GACGTGAACG GCCACAAGTT 4681 CTCTGTGCGG GGAGAGGGCG AAGGCGACGC CACAAATGGC AAGCTGACCC TGAAGTTCAT 4741 CTGCACCACC GGCAAGCTGC CCGTGCCTTG GCCTACCCTC GTGACCACAC TGACCTACGG 4801 CGTGCAGTGC TTCAGCAGAT ACCCCGACCA TATGAAGCGG CACGACTTCT TCAAGAGCGC 4861 CATGCCCGAG GGCTACGTGC AGGAACGGAC CATCAGCTTC AAGGACGACG GCACCTACAA 4921 GACCAGAGCC GAAGTGAAGT TCGAGGGCGA CACCCTCGTG AACCGGATCG AGCTGAAGGG 4981 CATCGATTTC AAAGAGGACG GCAACATCCT GGGCCACAAG CTGGAGTACA ACTTCAACAG 5041 CCACAACGTG TACATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT 5101 CCGGCACAAC GTGGAAGATG GCAGCGTGCA GCTGGCCGAC CACTACCAGC AGAACACCCC 5161 CATCGGAGAT GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGAGCGTGCT 5221 GAGCAAGGAC CCCAACGAGA AGCGGGACCA CATGGTGCTG CTGGAATTCG TGACCGCCGC 5281 TGGCATCACC CACGGCATGA GCGGCCTGAA CGACATCTTC GAGGCCCAGA AAATCGAGTG 5341 GCACGAGCAC CACCATCATC ACCATCACCA CTGATGATTC GAAAAATAAT AAGGATCCAA 5401 TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC 5461 TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT 5521 GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG 5581 GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG 5641 TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT 5701 TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT 5761 GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC 5821 CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA 5881 TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG 5941 AGATCTGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG 6001 CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT 6061 GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC 6121 AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ACTCGAGTTA AGGGCGAATT 6181 GCCGATTAGG ATCTTCCTAG AGCATGGCTA CGTAGATAAG TAGCATGGCG GGTTAATCAT 6241 TAACTACAAG GAACCCCTAG TGATGGAGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT 6301 CACTGAGGCC GGGCGACCAA AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT 6361 GAGCGAGCGA GCGCGCAGCC TTAATTAAGA GCTCATGGCG CGCCTAGGCC TTGACGGCCT 6421 TCCGCCAATT CGCCCTATAG TGAGTCGTAT TACGTCGCGC TCACTGGCCG TCGTTTTACA 6481 ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC 6541 TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAA ACGCCCTTCC CAACAGTTGC 6601 GCAGCCTGAA TGGCGAATGG GAGCGCCCTG TAGCGGCCAC TCAACCCTAT CTCGGTCTAT 6661 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 6721 TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTA G //