LOCUS CHST1-pET16-DEST 6964 bp DNA circular 28-JUN-2011 FEATURES Location/Qualifiers misc_feature 5323..5352 /note="His10-tag" misc_feature 5365..5376 /note="X-factor" misc_feature 5230..5245 /note="T7 Promoter" misc_feature 5249..5273 /note="Lac O" misc_feature 6691..6752 /note="T7 Terminator" misc_recomb complement(5392..5416) /label=attB1 /note="attB1 site" misc_recomb 6610..6632 /label=attB2 /note="attB2 site" misc_feature complement(3761..4720) /note="Lac I" misc_feature 209..1069 /note="Ap" misc_feature 1224..1843 /note="Ori" misc_feature 5417..5439 /note="TEV site" source 5440..6603 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:27060 IMAGE:4814499" /tissue_type="Brain, hippocampus" /clone_lib="NIH_MGC_95" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 5440..6603 /gene="CHST1" /gene_synonym=C6ST /gene_synonym=KS6ST /gene_synonym=KSGal6ST /db_xref="GeneID:8534" /db_xref="HGNC:1969" /db_xref="MIM:603797" CDS 5440..6603 /dnas_title="carbohydrate (keratan sulfate Gal-6) sulfotransferase 1" /gene="CHST1" /gene_synonym=C6ST /gene_synonym=KS6ST /gene_synonym=KSGal6ST /codon_start=1 /product="carbohydrate (keratan sulfate Gal-6) sulfotransferase 1" /protein_id="AAH22567.1" /db_xref="GI:21961240" /db_xref="GeneID:8534" /db_xref="HGNC:1969" /db_xref="MIM:603797" /translation="MQCSWKAVLLLALASIAIQYTAIRTFTAKSFHTCPGLAEAGLAE RLCEESPTFAYNLSRKTHILILATTRSGSSFVGQLFNQHLDVFYLFEPLYHVQNTLIP RFTQGKSPADRRVMLGASRDLLRSLYDCDLYFLENYIKPPPVNHTTDRIFRRGASRVL CSRPVCDPPGPADLVLEEGDCVRKCGLLNLTVAAEACRERSHVAIKTVRVPEVNDLRA LVEDPRLNLKVIQLVRDPRGILASRSETFRDTYRLWRLWYGTGRKPYNLDVTQLTTVC EDFSNSVSTGLMRPPWLKGKYMLVRYEDLARNPMKKTEEIYGFLGIPLDSHVARWIQN NTRGDPTLGKHKYGTVRNSAATAEKWRFRLSYDIVAFAQNACQQVLAQLGYKIAASEE ELKNPSVSLVEERDFRPFS" misc_difference 5583..5583 /gene="CHST1" /gene_synonym=C6ST /gene_synonym=KS6ST /gene_synonym=KSGal6ST /note="'C' in cDNA is 'T' in the human genome; no amino acid change." misc_feature 5440..6603 /note="CHST1 coding region" vector join(6618..6964,1..5399) /source="pET16-DEST" /type="Destination Vector" clone 5400..6617 /source="CHST1-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact 781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc 2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc 2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga 2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc 2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg 2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca 2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac 2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg 2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga 2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga 2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc 2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg 2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga 2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg 3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc 3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg 3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt 3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt 3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg 3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat 3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc 3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt 3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat 3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg 3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg 3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg 3721 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc 3781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 3841 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc 3901 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca 3961 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat 4021 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc 4081 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct 4141 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt 4201 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac 4261 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga 4321 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg 4381 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag 4441 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga 4501 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca 4561 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg 4621 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt 4681 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc 4741 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga 4801 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt 4861 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt 4921 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg 4981 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac 5041 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg 5101 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca 5161 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc 5221 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag 5281 aaataatttt gtttaacttt aagaaggaga tataccatgg gccatcatca tcatcatcat 5341 catcatcatc acagcagcgg ccatatcgaa ggtcgtcata tgctcgaATC AACAAGTTTG 5401 TACAAAAAAG CAGGCTctga aaacttgtac tttcaaggcc gcaccttcac cgccaagtcc 5461 tttcacacct gccccgggct ggcagaggcc gggctggccg agcgactgtg cgaggagagc 5521 cccaccttcg cctacaacct ctcccgcaag acccacatcc tcatcctggc caccacgcgc 5581 agcggctcct ccttcgtggg ccagctcttc aaccagcacc tggacgtctt ctacctgttt 5641 gagcccctct accacgtcca gaacacgctc atcccccgct tcacccaggg caagagcccg 5701 gccgaccggc gggtcatgct aggcgccagc cgcgacctcc tgcggagcct ctacgactgc 5761 gacctctact tcctggagaa ctacatcaag ccgccgccgg tcaaccacac caccgacagg 5821 atcttccgcc gcggggccag ccgggtcctc tgctcccggc ctgtgtgcga ccctccgggg 5881 ccagccgacc tggtcctgga ggagggggac tgtgtgcgca agtgcgggct actcaacctg 5941 accgtggcgg ccgaggcgtg ccgcgagcgc agccacgtgg ccatcaagac ggtgcgcgtg 6001 cccgaggtga acgacctgcg cgccctggtg gaagacccgc gattaaacct caaggtcatc 6061 cagctggtcc gagacccccg cggcattctg gcttcgcgca gcgagacctt ccgcgacacg 6121 taccggctct ggcggctctg gtacggcacc gggaggaaac cctacaacct ggacgtgacg 6181 cagctgacca cggtgtgcga ggacttctcc aactccgtgt ccaccggcct catgcggccc 6241 ccgtggctca agggcaagta catgttggtg cgctacgagg acctggctcg gaaccctatg 6301 aagaagaccg aggagatcta cgggttcctg ggcatcccgc tggacagcca cgtggcccgc 6361 tggatccaga acaacacgcg gggcgacccc accctgggca agcacaaata cggcaccgtg 6421 cgaaactcgg cggccacggc cgagaagtgg cgcttccgcc tctcctacga catcgtggcc 6481 tttgcccaga acgcctgcca gcaggtgctg gcccagctgg gctacaagat cgccgcctcg 6541 gaggaggagc tgaagaaccc ctcggtcagc ctggtggagg agcgggactt ccgccccttc 6601 tcgTAGGACC CAGCTTTCTT GTACAAAGTG GTTGATtcga ggatccggct gctaacaaag 6661 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg 6721 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc 6781 ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac 6841 ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta 6901 gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg 6961 agaa //