LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1676..1772) /label=attL2 /note="attL2 (c)" misc_signal complement(1787..1806) /note="T7 Promoter/priming site (c)" primer_bind 1814..1830 /note="M13 Reverse priming site" gene 1943..2752 /note="Kanamycin resistance gene" rep_origin 2873..3546 /note="pUC origin" vector join(1684..3549,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1669 /dnas_title="HS3ST2-Nterm-TEVfusion-pDONR221" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /chromosome="16" /map="16p12" /tissue_type="brain" /note="isolated by hybridization screening using IMAGE Consortium clone c-20d10 found in GenBank Accession Number F07258" gene 692..1669 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" CDS 692..1669 /dnas_title="heparan sulfate D-glucosaminyl 3-O-sulfotransferase-2" /gene="3OST2" /allele="3OST2-1" /EC_number="2.8.2.23" /note="3-OST-2" /codon_start=1 /product="heparan sulfate D-glucosaminyl 3-O-sulfotransferase-2" /protein_id="AAD30206.1" /db_xref="GI:4835719" /translation="MAYRVLGRAGPPQPRRARRLLFAFTLSLSCTYLCYSFLCCCDDL GRSRLLGAPRCLRGPSAGGQKLLQKSRPCDPSGPTPSEPSAPSAPAAAVPAPRLSGSN HSGSPKLGTKRLPQALIVGVKKGGTRAVLEFIRVHPDVRALGTEPHFFDRNYGRGLDW YRSLMPRTLESQITLEKTPSYFVTQEAPRRIFNMSRDTKLIVVVRNPVTRAISDYTQT LSKKPDIPTFEGLSFRNRTLGLVDVSWNAIRIGMYVLHLESWLQYFPLAQIHFVSGER LITDPAGEMGRVQDFLGIKRFITDKHFYFNKTKGFPCLKKTESSLLPRCLGKSKGRTH VQIDPEVIDQLREFYRPYNIKFYETVGQDFRWE" misc_feature 692..895 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="SPLAG domain; Ser, Pro, Leu, Ala, Gly enriched; other site" misc_feature 731..787 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative cystine bridged peptide loop; other site" misc_feature 803..805 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative O-glycosylation site" misc_feature 809..811 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative O-glycosylation site" misc_feature 818..820 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative O-glycosylation site" misc_feature 827..829 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative O-glycosylation site" misc_feature 863..871 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative N-glycosylation site" misc_feature 896..1669 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="sulfotransferase domain; other site" misc_feature 920..958 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative beta-strand/P-loop/alpha-helix motif; binds 5'-phosphate of 3'-phosphoadenosine 5'-phosphate, PAPS; binding site" misc_feature 1145..1153 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative N-glycosylation site" misc_feature 692..1669 /note="HS3ST2 coding region" misc_feature 1163..1210 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative sheet-loop-helix structure for binding the 3'-phosphate of PAPS" misc_feature 1226..1228 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative O-glycosylation site" misc_feature 1271..1279 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative N-glycosylation site" misc_feature 1319..1348 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative structure for binding 5'-sulfate of PAPS" misc_feature 1484..1492 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative N-glycosylation site" misc_feature 1505..1543 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative cystine bridged peptide loop; other site" misc_feature 1598..1606 /dnas_title="3OST2" /gene="3OST2" /allele="3OST2-1" /note="putative N-glycosylation site" variation 1300..1300 /dnas_title="3OST2" /gene="3OST2" /note="3OST2-2 allele; silent wobble position mutation" /replace="a" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgacgacctg ggtcggagcc gcctcctcgg 721 cgcgcctcgc tgcctccgcg gccccagcgc gggcggccag aaacttctcc agaagtcccg 781 cccctgtgat ccctccgggc cgacgcccag cgagcccagc gctcccagcg cgcccgccgc 841 cgccgtgccc gcccctcgcc tctccggttc caaccactcc ggctcaccca agctgggtac 901 caagcggttg ccccaagccc tcattgtggg cgtgaagaag gggggcaccc gggccgtgct 961 ggagtttatc cgagtacacc cggacgtgcg ggccttgggc acggaacccc acttctttga 1021 caggaactac ggccgcgggc tggattggta caggagcctg atgcccagga ccctcgagag 1081 ccagatcacg ctggagaaga cgcccagcta ctttgtcact caagaggctc ctcgacgcat 1141 cttcaacatg tcccgagaca ccaagctgat cgtggttgtg cggaaccctg tgacccgtgc 1201 catctctgat tacacgcaga cactctccaa gaagcccgac atcccgacct ttgagggcct 1261 ctccttccgc aaccgcaccc tgggcctggt ggacgtgtcg tggaacgcca tccgcatcgg 1321 catgtacgtg ctgcacctgg agagctggct gcagtacttc ccgctagctc agattcactt 1381 cgtcagtggc gagcgactca tcactgaccc ggccggcgag atggggcgag tccaggactt 1441 cctgggcatt aagagattca tcacggacaa gcacttctat ttcaacaaga ccaaaggatt 1501 cccttgcttg aaaaaaacag aatcgagcct cctgcctcga tgcttgggca aatcaaaagg 1561 gagaactcat gtacagattg atcctgaagt gatagaccag ctccgagaat tttatagacc 1621 gtataatatc aaattttatg aaaccgttgg gcaggacttc aggtgggaaT AGGACCCAGC 1681 TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA 1741 GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA GCTGATATCC CCTATAGTGA 1801 GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC AAAATCTCTG 1861 ATGTTACATT GCACAAGATA AAATAATATC ATCATGAACA ATAAAACTGT CTGCTTACAT 1921 AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCGA GGCCGCGATT 1981 AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA 2041 ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA 2101 ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT 2161 GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG 2221 GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA 2281 TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC 2341 TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG 2401 AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT 2461 TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC 2521 TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT 2581 TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG 2641 CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA 2701 TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT 2761 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC 2821 ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 2881 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 2941 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 3001 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC 3061 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 3121 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 3181 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 3241 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG 3301 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 3361 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 3421 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 3481 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 3541 TCACATGTT //