Home/GT Families/GT1/UGT85B1
UGT85B1
Sorghum bicolor · UDP-glucose:p-hydroxymandelonitrile O-glucosyltransferase (EC 2.4.1.85)
GT1Non-model organism · annotation 4–5Fold GT-BInverting2 PDB structure(s)
External resources
Identification and annotation
| CAZy family | GT1 · CAZy entry |
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| Gene symbol | UGT85B1 |
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| Synonyms / alternate names | - HMNGT
- Cyanohydrin beta-glucosyltransferase
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| UniProt accession | Q9SBL1 |
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| NCBI Gene ID | 8060874 |
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| Source species | Sorghum bicolor |
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| Taxonomic domain | Eukaryota |
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| UniProt annotation level | 5 |
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Function and localisation
| Enzyme function | UDP-glucose:p-hydroxymandelonitrile O-glucosyltransferase (EC 2.4.1.85) |
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| Subcellular location | - Cytoplasm
- Endoplasmic reticulum membrane
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Structure and mechanism
| Fold type | GT-B |
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| Catalytic mechanism | Inverting |
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| Cation dependence | No |
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| Oligomeric state | not curated |
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| PDB structures | 7ZER, 7ZF0 |
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Donor and acceptor specificity
| Sugar nucleotide donor | UDP-alpha-D-glucose |
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| Donor CCD code(s) | UPG |
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| Acceptor substrate(s) | (S)-4-hydroxymandelonitrile |
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| Acceptor CCD / GlycoCT | not curated |
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Protein sequences
Full protein sequence
492 aa
MGSNAPPPPTPHVVLVPFPGQGHVAPLMQLARLLHARGARVTFVYTQYNYRRLLRAKGEAAVRPPATSSARFRIEVIDDGLSLSVPQNDVGGLVDSLRKNCLHPFRALLRRLGQEVEGQDAPPVTCVVGDVVMTFAAAAAREAGIPEVQFFTASACGLLGYLHYGELVERGLVPFRDASLLADDDYLDTPLEWVPGMSHMRLRDMPTFCRTTDPDDVMVSATLQQMESAAGSKALILNTLYELEKDVVDALAAFFPPIYTVGPLAEVIASSDSASAGLAAMDISIWQEDTRCLSWLDGKPAGSVVYVNFGSMAVMTAAQAREFALGLASCGSPFLWVKRPDVVEGEEVLLPEALLDEVARGRGLVVPWCPQAAVLKHAAVGLFVSHCGWNSLLEATAAGQPVLAWPCHGEQTTNCRQLCEVWGNGAQLPREVESGAVARLVREMMVGDLGKEKRAKAAEWKAAAEAAARKGGASWRNVERVVNDLLLVGGKQ
Catalytic domain sequence
442 aa
PHVVLVPFPGQGHVAPLMQLARLLHARGARVTFVYTQYNYRRLLRAKGEAAVRPPATSSARFRIEVIDDGLSLSVPQNDVGGLVDSLRKNCLHPFRALLRRLGQEVEGQDAPPVTCVVGDVVMTFAAAAAREAGIPEVQFFTASACGLLGYLHYGELVERGLVPFRDASLLADDDYLDTPLEWVPGMSHMRLRDMPTFCRTTDPDDVMVSATLQQMESAAGSKALILNTLYELEKDVVDALAAFFPPIYTVGPLAEVIASSDSASAGLAAMDISIWQEDTRCLSWLDGKPAGSVVYVNFGSMAVMTAAQAREFALGLASCGSPFLWVKRPDVVEGEEVLLPEALLDEVARGRGLVVPWCPQAAVLKHAAVGLFVSHCGWNSLLEATAAGQPVLAWPCHGEQTTNCRQLCEVWGNGAQLPREVESGAVARLVREMMVGDLGKE
