Home/GT Families/GT13/MGAT1
MGAT1
Oryctolagus cuniculus · Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC 2.4.1.101)
GT13Non-model organism · annotation 4–5Fold GT-AInverting7 PDB structure(s)
External resources
Identification and annotation
| CAZy family | GT13 · CAZy entry |
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| Gene symbol | MGAT1 |
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| Synonyms / alternate names | - GNT1
- N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
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| UniProt accession | P27115 |
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| NCBI Gene ID | 100009521 |
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| Source species | Oryctolagus cuniculus |
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| Taxonomic domain | Eukaryota |
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| UniProt annotation level | 5 |
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Function and localisation
| Enzyme function | Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC 2.4.1.101) |
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| Subcellular location | - Golgi apparatus membrane
- Cytoplasm, perinuclear region
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Structure and mechanism
| Fold type | GT-A |
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| Catalytic mechanism | Inverting |
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| Cation dependence | Yes (Mn2+) |
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| Oligomeric state | Interacts with MGAT4D. Interacts with BRI3 (By similarity) |
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| PDB structures | 1FO8, 1FO9, 1FOA, 2AM3, 2AM4, 2AM5, 2APC |
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Donor and acceptor specificity
| Sugar nucleotide donor | UDP-N-acetyl-alpha-D-glucosamine |
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| Donor CCD code(s) | UD1 |
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| Acceptor substrate(s) | - N(4)-(alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)
- an N(4)-{alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein]
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| Acceptor CCD / GlycoCT | not curated |
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Protein sequences
Full protein sequence
447 aa
MLKKQSAGLVLWGAILFVAWNALLLLFFWTRPVPSRLPSDNALDDDPASLTREVIRLAQDAEVELERQRGLLQQIREHHALWSQRWKVPTAAPPAQPHVPVTPPPAVIPILVIACDRSTVRRCLDKLLHYRPSAELFPIIVSQDCGHEETAQVIASYGSAVTHIRQPDLSNIAVQPDHRKFQGYYKIARHYRWALGQIFHNFNYPAAVVVEDDLEVAPDFFEYFQATYPLLKADPSLWCVSAWNDNGKEQMVDSSKPELLYRTDFFPGLGWLLLAELWAELEPKWPKAFWDDWMRRPEQRKGRACVRPEISRTMTFGRKGVSHGQFFDQHLKFIKLNQQFVPFTQLDLSYLQQEAYDRDFLARVYGAPQLQVEKVRTNDRKELGEVRVQYTGRDSFKAFAKALGVMDDLKSGVPRAGYRGIVTFLFRGRRVHLAPPQTWDGYDPSWT
Catalytic domain sequence
340 aa
IPILVIACDRSTVRRCLDKLLHYRPSAELFPIIVSQDCGHEETAQVIASYGSAVTHIRQPDLSNIAVQPDHRKFQGYYKIARHYRWALGQIFHNFNYPAAVVVEDDLEVAPDFFEYFQATYPLLKADPSLWCVSAWNDNGKEQMVDSSKPELLYRTDFFPGLGWLLLAELWAELEPKWPKAFWDDWMRRPEQRKGRACVRPEISRTMTFGRKGVSHGQFFDQHLKFIKLNQQFVPFTQLDLSYLQQEAYDRDFLARVYGAPQLQVEKVRTNDRKELGEVRVQYTGRDSFKAFAKALGVMDDLKSGVPRAGYRGIVTFLFRGRRVHLAPPQTWDGYDPSWT
