Home/GT Families/GT13/Mgat1
Mgat1
Drosophila melanogaster · Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC 2.4.1.101)
GT13Model organism · annotation ≥ 4Fold GT-AInverting
External resources
Identification and annotation
| CAZy family | GT13 · CAZy entry |
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| Gene symbol | Mgat1 |
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| Synonyms / alternate names | N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I |
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| UniProt accession | Q9NGK4 |
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| NCBI Gene ID | not curated |
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| Source species | Drosophila melanogaster |
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| Taxonomic domain | Eukaryota |
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| UniProt annotation level | 4 |
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Function and localisation
| Enzyme function | Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC 2.4.1.101) |
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| Subcellular location | - Cytoplasm, perinuclear region
- Golgi apparatus membrane
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Structure and mechanism
| Fold type | GT-A |
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| Catalytic mechanism | Inverting |
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| Cation dependence | Yes (Mn2+) |
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| Oligomeric state | not curated |
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| PDB structures | not curated |
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Donor and acceptor specificity
| Sugar nucleotide donor | UDP-N-acetyl-alpha-D-glucosamine |
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| Donor CCD code(s) | UD1 |
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| Acceptor substrate(s) | - N(4)-(alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1
- 2)
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| Acceptor CCD / GlycoCT | not curated |
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Protein sequences
Full protein sequence
456 aa
MRTRKVLLVIGFLVTWTYATYYLLLRQTGIHTSRHQSLQAYKLNSQARDANMQSHHLAKNVFEFVKLKYLEKQPPSVASTPQISIIAAEISAELPEQHVAKSATARIPTKTYLANGEPVFPVVVFACNRVSVKKCIDNLVQYRPSVEQFPIIVSQDCGDEPTKEAILSYGKQVTLIEQPDLSDIMPKEKKFKGYYKIARHYGWALNTTFAVGFEFVIIVEDDLNVAPDFFEYFLGTHKLLKQDPSLWCVSAWNDNGKAAVVDAAQPELLYRTDFFPGLGWMLTKDLWAELSVKWPKSFWDDWIRHPAQRKDRVCIRPEISRTRTFGKIGVSNGLFFDKYLKHIKLSEDFVQFTKINMSYLLKDNYDNTFLRRVYTYPIVTYDELRRNLIRIEGPVRIQYTTREQYKRTTKMLGLMDDFKSGVPRTAYHGIVSFYYNKRRVHLAPNANWKGYELSWS
Catalytic domain sequence
329 aa
VFPVVVFACNRVSVKKCIDNLVQYRPSVEQFPIIVSQDCGDEPTKEAILSYGKQVTLIEQPDLSDIMPKEKKFKGYYKIARHYGWALNTTFAVGFEFVIIVEDDLNVAPDFFEYFLGTHKLLKQDPSLWCVSAWNDNGKAAVVDAAQPELLYRTDFFPGLGWMLTKDLWAELSVKWPKSFWDDWIRHPAQRKDRVCIRPEISRTRTFGKIGVSNGLFFDKYLKHIKLSEDFVQFTKINMSYLLKDNYDNTFLRRVYTYPIVTYDELRRNLIRIEGPVRIQYTTREQYKRTTKMLGLMDDFKSGVPRTAYHGIVSFYYNKRRVHLAPNAN
