Home/GT Families/GT14/GCNT1
GCNT1
Homo sapiens · Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase (EC 2.4.1.102)
GT14Model organism · annotation ≥ 4Fold GT-AInverting
External resources
Identification and annotation
| CAZy family | GT14 · CAZy entry |
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| Gene symbol | GCNT1 |
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| Synonyms / alternate names | - NACGT2
- Core 2 beta-1,6-N-acetylglucosaminyltransferase
- Core 2-branching enzyme
- Core2-GlcNAc-transferase
- Leukocyte type core 2 beta-1,6-N-acetylglucosaminyltransferase
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| UniProt accession | Q02742 |
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| NCBI Gene ID | 2650 |
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| Source species | Homo sapiens |
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| Taxonomic domain | Eukaryota |
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| UniProt annotation level | 5 |
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Function and localisation
| Enzyme function | Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase (EC 2.4.1.102) |
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| Subcellular location | Golgi apparatus membrane |
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Structure and mechanism
| Fold type | GT-A |
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| Catalytic mechanism | Inverting |
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| Cation dependence | No |
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| Oligomeric state | Interacts with GOLPH3; may control GCNT1 retention in the Go |
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| PDB structures | not curated |
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Donor and acceptor specificity
| Sugar nucleotide donor | UDP-N-acetyl-alpha-D-glucosamine |
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| Donor CCD code(s) | UD1 |
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| Acceptor substrate(s) | - a 3-O-[beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein]
- a 3-O-[beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein]
- a globoside GalGb4Cer
- a ganglioside GA1
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| Acceptor CCD / GlycoCT | not curated |
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Protein sequences
Full protein sequence
428 aa
MLRTLLRRRLFSYPTKYYFMVLVLSLITFSVLRIHQKPEFVSVRHLELAGENPSSDINCTKVLQGDVNEIQKVKLEILTVKFKKRPRWTPDDYINMTSDCSSFIKRRKYIVEPLSKEEAEFPIAYSIVVHHKIEMLDRLLRAIYMPQNFYCIHVDTKSEDSYLAAVMGIASCFSNVFVASRLESVVYASWSRVQADLNCMKDLYAMSANWKYLINLCGMDFPIKTNLEIVRKLKLLMGENNLETERMPSHKEERWKKRYEVVNGKLTNTGTVKMLPPLETPLFSGSAYFVVSREYVGYVLQNEKIQKLMEWAQDTYSPDEYLWATIQRIPEVPGSLPASHKYDLSDMQAVARFVKWQYFEGDVSKGAPYPPCDGVHVRSVCIFGAGDLNWMLRKHHLFANKFDVDVDLFAIQCLDEHLRHKALETLKH
Catalytic domain sequence
269 aa
IAYSIVVHHKIEMLDRLLRAIYMPQNFYCIHVDTKSEDSYLAAVMGIASCFSNVFVASRLESVVYASWSRVQADLNCMKDLYAMSANWKYLINLCGMDFPIKTNLEIVRKLKLLMGENNLETERMPSHKEERWKKRYEVVNGKLTNTGTVKMLPPLETPLFSGSAYFVVSREYVGYVLQNEKIQKLMEWAQDTYSPDEYLWATIQRIPEVPGSLPASHKYDLSDMQAVARFVKWQYFEGDVSKGAPYPPCDGVHVRSVCIFGAGDLNWM
