Home/GT Families/GT14/Gcnt1
Gcnt1
Mus musculus · Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase (EC 2.4.1.102)
GT14Non-model organism · annotation 4–5Fold GT-AInverting3 PDB structure(s)
External resources
Identification and annotation
| CAZy family | GT14 · CAZy entry |
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| Gene symbol | Gcnt1 |
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| Synonyms / alternate names | - Core 2 beta-1,6-N-acetylglucosaminyltransferase
- Core 2-branching enzyme
- Core2-GlcNAc-transferase
- Leukocyte type core 2 beta-1,6-N-acetylglucosaminyltransferase
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| UniProt accession | Q09324 |
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| NCBI Gene ID | 14537 |
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| Source species | Mus musculus |
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| Taxonomic domain | Eukaryota |
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| UniProt annotation level | 5 |
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Function and localisation
| Enzyme function | Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase (EC 2.4.1.102) |
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| Subcellular location | Golgi apparatus membrane |
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Structure and mechanism
| Fold type | GT-A |
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| Catalytic mechanism | Inverting |
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| Cation dependence | No |
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| Oligomeric state | Interacts with GOLPH3; may control GCNT1 retention in the Go |
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| PDB structures | 2GAK, 2GAM, 3OTK |
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Donor and acceptor specificity
| Sugar nucleotide donor | UDP-N-acetyl-alpha-D-glucosamine |
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| Donor CCD code(s) | UD1 |
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| Acceptor substrate(s) | - a 3-O-[beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein]
- a 3-O-[beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein]
- a globoside GalGb4Cer
- a ganglioside GA1
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| Acceptor CCD / GlycoCT | not curated |
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Protein sequences
Full protein sequence
428 aa
MLRNLFRRRLFSCPTKYYFMLLVLSLITFSVLRIHQKPEFFSVRHLELAGDDPYSNVNCTKILQGDPEEIQKVKLEILTVQFKKRPRRTPHDYINMTRDCASFIRTRKYIVEPLTKEEVGFPIAYSIVVHHKIEMLDRLLRAIYMPQNFYCIHVDRKAEESFLAAVQGIASCFDNVFVASQLESVVYASWSRVKADLNCMKDLYRMNANWKYLINLCGMDFPIKTNLEIVRKLKCSTGENNLETEKMPPNKEERWKKRYTVVDGKLTNTGIVKAPPPLKTPLFSGSAYFVVTREYVGYVLENENIQKLMEWAQDTYSPDEFLWATIQRIPEVPGSFPSSNKYDLSDMNAIARFVKWQYFEGHVSNGAPYPPCSGVHVRSVCVFGAGDLSWMLRQHHLFANKFDMDVDPFAIQCLDEHLRHKALENLEH
Catalytic domain sequence
269 aa
IAYSIVVHHKIEMLDRLLRAIYMPQNFYCIHVDRKAEESFLAAVQGIASCFDNVFVASQLESVVYASWSRVKADLNCMKDLYRMNANWKYLINLCGMDFPIKTNLEIVRKLKCSTGENNLETEKMPPNKEERWKKRYTVVDGKLTNTGIVKAPPPLKTPLFSGSAYFVVTREYVGYVLENENIQKLMEWAQDTYSPDEFLWATIQRIPEVPGSFPSSNKYDLSDMNAIARFVKWQYFEGHVSNGAPYPPCSGVHVRSVCVFGAGDLSWM
