Home/GT Families/GT21/GCS1
GCS1
Cryptococcus neoformans (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) · Ceramide glucosyltransferase (EC 2.4.1.80)
GT21Non-model organism · annotation 4–5Fold GT-AInverting
External resources
Identification and annotation
| CAZy family | GT21 · CAZy entry |
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| Gene symbol | GCS1 |
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| Synonyms / alternate names | - GLCT-1
- Glucosylceramide synthase
- UDP-glucose ceramide glucosyltransferase
- UDP-glucose:N-acylsphingosine D-glucosyltransferase
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| UniProt accession | J9W453 |
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| NCBI Gene ID | 23888885 |
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| Source species | Cryptococcus neoformans (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) |
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| Taxonomic domain | Eukaryota |
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| UniProt annotation level | 4 |
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Function and localisation
| Enzyme function | Ceramide glucosyltransferase (EC 2.4.1.80) |
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| Subcellular location | Golgi apparatus membrane |
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Structure and mechanism
| Fold type | GT-A |
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| Catalytic mechanism | Inverting |
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| Cation dependence | No |
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| Oligomeric state | not curated |
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| PDB structures | not curated |
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Donor and acceptor specificity
| Sugar nucleotide donor | UDP-alpha-D-glucose |
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| Donor CCD code(s) | UPG |
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| Acceptor substrate(s) | an N-acylsphing-4-enine |
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| Acceptor CCD / GlycoCT | not curated |
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Protein sequences
Full protein sequence
450 aa
MSDSGTLSLIGGIVFLVLWVVVWSICLLGWRTARIRYAHPNIPSRLSKLPVSSAPGVTIIRPLCGLDQNLYNTLESVMKLDYPKFEVIFAVQDEKDEALPVVNMVMEKYPEVEAKVIIDSRKVGVNPKVNNLMTPFQEAKYDLLWILDSTCSVLPGTLGRSVEAFFSNTSSTASPYDPESSPLLSISDDVRKPPIAGEVGLVHQVPIAVCYQKTWGSLIEQAYLNTTHAKMYLAINATSIDSCVVGKSCMYSRDNISHLTTPSPSLRSLPDPPSGLAGFGPFLAEDNMIGLSLWHELKLKHAMTSDVVLDFIGSLSVRDYINRRVRWIRVRKKMTLAATLLEPLTESIISGLYGAWAISRLLGGNILPLFLLHMAAWISVDISTKRALETNIKGIGPPESKVTFLMAWAARECLALPIWMLAMTSSEVVWRGQKYKIIASGEAIRLGDRN
Catalytic domain sequence
418 aa
VWSICLLGWRTARIRYAHPNIPSRLSKLPVSSAPGVTIIRPLCGLDQNLYNTLESVMKLDYPKFEVIFAVQDEKDEALPVVNMVMEKYPEVEAKVIIDSRKVGVNPKVNNLMTPFQEAKYDLLWILDSTCSVLPGTLGRSVEAFFSNTSSTASPYDPESSPLLSISDDVRKPPIAGEVGLVHQVPIAVCYQKTWGSLIEQAYLNTTHAKMYLAINATSIDSCVVGKSCMYSRDNISHLTTPSPSLRSLPDPPSGLAGFGPFLAEDNMIGLSLWHELKLKHAMTSDVVLDFIGSLSVRDYINRRVRWIRVRKKMTLAATLLEPLTESIISGLYGAWAISRLLGGNILPLFLLHMAAWISVDISTKRALETNIKGIGPPESKVTFLMAWAARECLALPIWMLAMTSSEVVWRGQKYKIIA
