st6gal1 (GT29)

st6gal1

Monodelphis domestica · Beta-galactoside alpha-2,6-sialyltransferase 1 (EC 2.4.3.1)

GT29Non-model organism · annotation 4–5Fold GT-AInverting

External resources

UniProt E4ZFH4 AlphaFold model InterPro domains NCBI Gene 100617174 CAZy GT29

Identification and annotation

CAZy family	GT29 · CAZy entry
Gene symbol	st6gal1
Synonyms / alternate names	CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1 ST6Gal I Sialyltransferase 1
UniProt accession	`E4ZFH4`
NCBI Gene ID	`100617174`
Source species	Monodelphis domestica
Taxonomic domain	Eukaryota
UniProt annotation level	4

Function and localisation

Enzyme function	Beta-galactoside alpha-2,6-sialyltransferase 1 (EC 2.4.3.1)
Subcellular location	Golgi apparatus, Golgi stack membrane Secreted

Structure and mechanism

Fold type	GT-A
Catalytic mechanism	Inverting
Cation dependence	No
Oligomeric state	not curated
PDB structures	not curated

Donor and acceptor specificity

Sugar nucleotide donor	CMP-N-acetyl-beta-neuraminate
Donor CCD code(s)	`NCC`
Acceptor substrate(s)	a beta-D-galactoside an N(4)-{alpha-Neu5Ac-(2->6)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->6)]-beta-D-GlcNAc}-L-asparaginyl-[protein] an N(4)-{alpha-Neu5Ac-(2->6)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] an N(4)-{alpha-Neu5Ac-(2->6)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]-[beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->6)]-beta-D-GlcNAc}-L-asparaginyl-[protein] an N(4)-{beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->6)]-beta-D-GlcNAc}-L-asparaginyl-[protein] an N(4)-{beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] an N(4)-{beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]-[alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->6)]-beta-D-GlcNAc}-L-asparaginyl-[protein] an N(4)-{beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]-[beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->6)]-beta-D-GlcNAc}-L-asparaginyl-[protein] an N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->6)]-beta-D-GlcNAc}-L-asparaginyl-[protein] an N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]-[beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->6)]-beta-D-GlcNAc}-L-asparaginyl-[protein]
Acceptor CCD / GlycoCT	not curated

Protein sequences

Full protein sequence

401 aa

MVHINFFRRIMYGLLAFLLFLTICLWNESKKGYSVSFRLDSKGFQLPRTLENLSRRLRTKPVNITAMVGPQRESEAPRPRPKVDKFSYKVWDKDSSSKNLPARLQKVRKNYLHMNKYNVSFEGTRQVEKLSTSGLLCQLANRVKVTMIQGTDFPFNTSEWEKSLPPKNIRESFGRLGTCAVVSSAGSLKASYLGTEIDNHDAVLRFNGAPTKKYEEHVGKKTTFRLVNSQLVTTDKNFFDDSLYKNGILIMWDPSPYHSEIPEWYKKPDYNFFHNYKLYRKLNPNQPFYILNPKMPWELWDILQENSPEDIQPNPPSSGMIGIVIMMNFCDTVDIYEFLPSKRKTDICHYYQQFFDSACTMGAYHPLLFEKNMVKHLNQGTDEDIYLKGKVTVPGLQGVRC

Catalytic domain sequence

208 aa

SFGRLGTCAVVSSAGSLKASYLGTEIDNHDAVLRFNGAPTKKYEEHVGKKTTFRLVNSQLVTTDKNFFDDSLYKNGILIMWDPSPYHSEIPEWYKKPDYNFFHNYKLYRKLNPNQPFYILNPKMPWELWDILQENSPEDIQPNPPSSGMIGIVIMMNFCDTVDIYEFLPSKRKTDICHYYQQFFDSACTMGAYHPLLFEKNMVKHLNQ