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Glycosyltransferase Curated Online Resource for Enzymology

Family-organised sequence, structure, mechanism and substrate-specificity records for the CAZy glycosyltransferases

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A4GALT

Homo sapiens · Lactosylceramide 4-alpha-galactosyltransferase (EC 2.4.1.228)

GT32Model organism · annotation ≥ 4Fold GT-ARetaining

External resources

UniProt Q9NPC4AlphaFold modelInterPro domainsNCBI Gene 53947CAZy GT32

Identification and annotation

CAZy familyGT32 · CAZy entry
Gene symbolA4GALT
Synonyms / alternate names
  • A14GALT
  • A4GALT1
  • Alpha-1,4-N-acetylglucosaminyltransferase
  • Alpha-1,4-galactosyltransferase
  • Alpha4Gal-T1
  • CD77 synthase
  • Globotriaosylceramide synthase
  • P1/Pk synthase
  • UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase
UniProt accessionQ9NPC4
NCBI Gene ID53947
Source speciesHomo sapiens
Taxonomic domainEukaryota
UniProt annotation level5

Function and localisation

Enzyme functionLactosylceramide 4-alpha-galactosyltransferase (EC 2.4.1.228)
Subcellular locationGolgi apparatus membrane

Structure and mechanism

Fold typeGT-A
Catalytic mechanismRetaining
Cation dependenceYes (Mn2+)
Oligomeric statenot curated
PDB structuresnot curated

Donor and acceptor specificity

Sugar nucleotide donor
  • UDP-alpha-D-galactose
  • 2 UDP-alpha-D-galactose
Donor CCD code(s)GDU
Acceptor substrate(s)
  • a beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-Cer(d18:1(4E))
  • an N(4)-{beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein]
  • a globoside Gb4Cer
  • a beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
  • a beta-D-Gal-(1<->1')-ceramide
Acceptor CCD / GlycoCTnot curated

Protein sequences

Full protein sequence

353 aa 
MSKPPDLLLRLLRGAPRQRVCTLFIIGFKFTFFVSIMIYWHVVGEPKEKGQLYNLPAEIPCPTLTPPTPPSHGPTPGNIFFLETSDRTNPNFLFMCSVESAARTHPESHVLVLMKGLPGGNASLPRHLGISLLSCFPNVQMLPLDLRELFRDTPLADWYAAVQGRWEPYLLPVLSDASRIALMWKFGGIYLDTDFIVLKNLRNLTNVLGTQSRYVLNGAFLAFERRHEFMALCMRDFVDHYNGWIWGHQGPQLLTRVFKKWCSIRSLAESRACRGVTTLPPEAFYPIPWQDWKKYFEDINPEELPRLLSATYAVHVWNKKSQGTRFEATSRALLAQLHARYCPTTHEAMKMYL

Catalytic domain sequence

127 aa 
AFERRHEFMALCMRDFVDHYNGWIWGHQGPQLLTRVFKKWCSIRSLAESRACRGVTTLPPEAFYPIPWQDWKKYFEDINPEELPRLLSATYAVHVWNKKSQGTRFEATSRALLAQLHARYCPTTHEA
All GT32 recordsA4GNT →