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Glycosyltransferase Curated Online Resource for Enzymology

Family-organised sequence, structure, mechanism and substrate-specificity records for the CAZy glycosyltransferases

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Mgat4a

Mus musculus · Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (EC 2.4.1.145)

GT54Non-model organism · annotation 4–5Fold GT-AInverting1 PDB structure(s)

External resources

UniProt Q812G0AlphaFold modelInterPro domainsNCBI Gene 269181CAZy GT54PDB 7VMT

Identification and annotation

CAZy familyGT54 · CAZy entry
Gene symbolMgat4a
Synonyms / alternate names
  • N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa
  • UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVa
UniProt accessionQ812G0
NCBI Gene ID269181
Source speciesMus musculus
Taxonomic domainEukaryota
UniProt annotation level5

Function and localisation

Enzyme functionAlpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (EC 2.4.1.145)
Subcellular locationGolgi apparatus membrane

Structure and mechanism

Fold typeGT-A
Catalytic mechanismInverting
Cation dependenceYes (Mn2+)
Oligomeric statenot curated
PDB structures7VMT

Donor and acceptor specificity

Sugar nucleotide donorUDP-N-acetyl-alpha-D-glucosamine
Donor CCD code(s)UD1
Acceptor substrate(s)
  • N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein]
  • an N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein]
  • an N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein]
  • an N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->6)]-beta-D-GlcNAc}-L-asparaginyl-[protein]
  • an N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein]
  • N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-{alpha-D-Man-(1->6)}-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-asparaginyl-[protein]
  • N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-asparaginyl-[protein]
Acceptor CCD / GlycoCTnot curated

Protein sequences

Full protein sequence

535 aa 
MRLRNGTVATALVFVTSFLTLSWYTTWQNGKEKLIAYQREFLALKERLRVAEHRISQRSSELNTIVQQFRRAGAETNGSKTALSTISDNTIKLLKELTSKKSLRVPSIYYHLPHLLQNERSLQPAVQIGSGRTGVSIVMGIPTVKREVKSYLVETLHSLIDNLYPEEKLDCVIVVFIGETDLDYVHSVVANLEKEFSREISSGLLEIISPPESYYPDLTNLKETFGDSKERVRWRTKQNLDYCFLMMYAQEKGIYYIQLEDDIIVKQNYFNTIKNFALQLSSEEWMILEFSQLGFIGKMFQAPDLALVVEFILMFYKEKPIDWLLDHILWVKVCNPEKDAKHCDRQKANLRIRFRPSLFQHVGLHSSLSGKIQKLTDKDYMKPLLLKVHVNPPAEVSTSLKVYQGHTLEKTYMGEDFFWAITPTAGDYILFKFDKPVNVESYLFHSGNQEHPGDILLNTTVDVLPLKSDSLEISKETKDKRLEDGYFRIGKFEYGVAEGIVDPGLNPISAFRLSVIQNSAVWAILNEIHIKKVTS

Catalytic domain sequence

277 aa 
RVPSIYYHLPHLLQNERSLQPAVQIGSGRTGVSIVMGIPTVKREVKSYLVETLHSLIDNLYPEEKLDCVIVVFIGETDLDYVHSVVANLEKEFSREISSGLLEIISPPESYYPDLTNLKETFGDSKERVRWRTKQNLDYCFLMMYAQEKGIYYIQLEDDIIVKQNYFNTIKNFALQLSSEEWMILEFSQLGFIGKMFQAPDLALVVEFILMFYKEKPIDWLLDHILWVKVCNPEKDAKHCDRQKANLRIRFRPSLFQHVGLHSSLSGKIQKLTDKDY
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