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DIE2
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) · Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase (EC 2.4.1.256)
GT59 Model organism · annotation ≥ 4 Fold GT-C Inverting
External resources
Identification and annotation
CAZy family GT59 · CAZy entry Gene symbol DIE2 Synonyms / alternate names ALG10 Alpha-1,2-glucosyltransferase ALG10-A Alpha-2-glucosyltransferase ALG10 Asparagine-linked glycosylation protein 10 Dolichyl-phosphoglucose-dependent glucosyltransferase ALG10 UniProt accession P50076NCBI Gene ID 853142Source species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Taxonomic domain Eukaryota UniProt annotation level 5
Function and localisation
Enzyme function Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase (EC 2.4.1.256) Subcellular location Endoplasmic reticulum membrane
Structure and mechanism
Fold type GT-C Catalytic mechanism Inverting Cation dependence No Oligomeric state not curated PDB structures not curated
Donor and acceptor specificity
Sugar nucleotide donor not curated Donor CCD code(s) not curated Acceptor substrate(s) an alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate Acceptor CCD / GlycoCT not curated
Protein sequences
Full protein sequence
525 aa
Copy
FASTA
MDAKKNTGEANNDVLEEEAAIQLIAPGIARNLTQEVITGIFCNVVIYPLLLIYFVLTFRYMTTNIVPYEFIDEKFHVGQTLTYLKGKWTQWDPKITTPPGIYILGLINYYCIKPIFKSWSTLTILRLVNLLGGIIVFPILVLRPIFLFNALGFWPVSLMSFPLMTTYYYLFYTDVWSTILILQSLSCVLTLPFGPVKSIWLSAFFAGVSCLFRQTNIIWTGFIMILAVERPAILQKQFNTHTFNNYLKLFIHAIDDFSNLVLPYMINFVLFFIYLIWNRSITLGDKSSHSAGLHIVQIFYCFTFITVFSLPIWISRNFMKLYKLRIKRKPVQTFFEFIGIMLIIRYFTKVHPFLLADNRHYTFYLFRRLIGNKSRLIKYFFMTPIYHFSTFAYLEVMRPNQLTFHPITPLPIKEPVHLPIQLTHVSWTALITCTMVTIVPSPLFEPRYYILPYFFWRIFITCSCEPLIKDLKPAKEGENPITISSTKRLFMEFLWFMLFNVVTLVIFSKVSFPWTTEPYLQRIIW
Catalytic domain sequence
393 aa
Copy
FASTA
VPYEFIDEKFHVGQTLTYLKGKWTQWDPKITTPPGIYILGLINYYCIKPIFKSWSTLTILRLVNLLGGIIVFPILVLRPIFLFNALGFWPVSLMSFPLMTTYYYLFYTDVWSTILILQSLSCVLTLPFGPVKSIWLSAFFAGVSCLFRQTNIIWTGFIMILAVERPAILQKQFNTHTFNNYLKLFIHAIDDFSNLVLPYMINFVLFFIYLIWNRSITLGDKSSHSAGLHIVQIFYCFTFITVFSLPIWISRNFMKLYKLRIKRKPVQTFFEFIGIMLIIRYFTKVHPFLLADNRHYTFYLFRRLIGNKSRLIKYFFMTPIYHFSTFAYLEVMRPNQLTFHPITPLPIKEPVHLPIQLTHVSWTALITCTMVTIVPSPLFEPRYYILPYFFWRI
