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EXT1
Cricetulus griseus · Exostosin-1 (EC 2.4.1.225)
GT64Non-model organism · annotation 4–5Fold GT-ARetaining
External resources
Identification and annotation
| CAZy family | GT64 · CAZy entry |
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| Gene symbol | EXT1 |
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| Synonyms / alternate names | - Heparan sulfate copolymerase
- N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase
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| UniProt accession | Q9JK82 |
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| NCBI Gene ID | 100689334 |
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| Source species | Cricetulus griseus |
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| Taxonomic domain | Eukaryota |
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| UniProt annotation level | 5 |
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Function and localisation
| Enzyme function | Exostosin-1 (EC 2.4.1.225) |
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| Subcellular location | - Golgi apparatus membrane
- Golgi apparatus, cis-Golgi network membrane
- Endoplasmic reticulum membrane
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Structure and mechanism
| Fold type | GT-A |
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| Catalytic mechanism | Retaining |
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| Cation dependence | Yes (Mn2+) |
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| Oligomeric state | Part of the heparan sulfate polymerase, a dimeric complex co |
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| PDB structures | not curated |
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Donor and acceptor specificity
| Sugar nucleotide donor | UDP-alpha-D-glucuronate |
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| Donor CCD code(s) | UGA |
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| Acceptor substrate(s) | 3-O-{alpha-D-GlcNAc-[(1->4)-beta-D-GlcA-(1->4)-alpha-D-GlcNAc](n)-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl}-L-seryl-[protein] |
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| Acceptor CCD / GlycoCT | not curated |
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Protein sequences
Full protein sequence
746 aa
MQAKKRYFILLSAGSCLALLFYFGGVQFRASRSHSRREEHSGRNGLHQPSPDHFWPRFADALHPFFPWDQLENEDSGVHVSPRQKRDANSSVYKGKKCRMESCFDFALCKKNGFKVYVYPQQKGEKIAESYQNILAAIEGSRFYTSDPSQACLFVLSLDTLDRDQLSPQYVHNLRSKVQSLHLWNNGRNHLIFNLYSGTWPDYTEDVGFDIGQAMLAKASISTENFRPNFDVSIPLFSKDHPRTGGERGFLKFNTIPPLRKYMLVFKGKRYLTGIGSDTRNALYHVHNGEDVLLLTTCKHGKDWQKHKDSRCDRDNTEYEKYDYREMLHNATFCLVPRGRRLGSFRFLEALQAACVPVMLSNGWELPFSEVINWNQAAVIGDERLLLQIPSTIRSIHQDKILALRQQTQFLWEAYFSSVEKIVLTTLEIIQDRIFKHISRNSLIWNKHPGGLFVLPQYSSYLGDFPYYYANLGLKPPSKFTAVIHAVTPLVSQSQPVLKLLVAAAKSQYCAQIIVLWNCDKPLPAKHRWPATAVPVIVIEGESKVMSSRFLPYDNIITDAVLSLDEDTVLSTTEVDFAFTVWQSFPERIVGYPARSHFWDNSKERWGYTSKWTNDYSMVLTGAAIYHKYYHYLYTHYLPASLKNMVDQLANCEDILMNFLVSAVTKLPPIKVTQKKQYKETMMGQTSRASRWADPDHFAQRQSCMNTFASWFGYMPLIHSQMRLDPVLFKDQVSILRKKYRDIERL
Catalytic domain sequence
285 aa
KNGFKVYVYPQQKGEKIAESYQNILAAIEGSRFYTSDPSQACLFVLSLDTLDRDQLSPQYVHNLRSKVQSLHLWNNGRNHLIFNLYSGTWPDYTEDVGFDIGQAMLAKASISTENFRPNFDVSIPLFSKDHPRTGGERGFLKFNTIPPLRKYMLVFKGKRYLTGIGSDTRNALYHVHNGEDVLLLTTCKHGKDWQKHKDSRCDRDNTEYEKYDYREMLHNATFCLVPRGRRLGSFRFLEALQAACVPVMLSNGWELPFSEVINWNQAAVIGDERLLLQIPSTIRS
