Pofut1 (GT65)

Pofut1

Mus musculus · GDP-fucose protein O-fucosyltransferase 1 (EC 2.4.1.221)

GT65Non-model organism · annotation 4–5Fold GT-BInverting10 PDB structure(s)

External resources

UniProt Q91ZW2 AlphaFold model InterPro domains NCBI Gene 140484 CAZy GT65 PDB 5KXH PDB 5KXQ PDB 5KY0 PDB 5KY2 PDB 5KY3 PDB 5KY4 PDB 5KY5 PDB 5KY7

Identification and annotation

CAZy family	GT65 · CAZy entry
Gene symbol	Pofut1
Synonyms / alternate names	Peptide-O-fucosyltransferase 1
UniProt accession	`Q91ZW2`
NCBI Gene ID	`140484`
Source species	Mus musculus
Taxonomic domain	Eukaryota
UniProt annotation level	5

Function and localisation

Enzyme function	GDP-fucose protein O-fucosyltransferase 1 (EC 2.4.1.221)
Subcellular location	Endoplasmic reticulum

Structure and mechanism

Fold type	GT-B
Catalytic mechanism	Inverting
Cation dependence	No
Oligomeric state	not curated
PDB structures	`5KXH`, `5KXQ`, `5KY0`, `5KY2`, `5KY3`, `5KY4`, `5KY5`, `5KY7`, `5KY8`, `5KY9`

Donor and acceptor specificity

Sugar nucleotide donor	GDP-beta-L-fucose
Donor CCD code(s)	`GFB`
Acceptor substrate(s)	L-seryl-[protein] L-threonyl-[protein]
Acceptor CCD / GlycoCT	not curated

Protein sequences

Full protein sequence

393 aa

MGAAAWAPPHLLLRASFLLLLLLLPLRGRSAGSWDLAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKPPFTNLHVSYQKYFKLEPLQAYHRVVSLEDFMENLAPSHWPPEKRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELFTGISFSASYKEQWTQRFPAKEHPVLALPGAPAQFPVLEEHRELQKYMVWSDEMVRTGEALISAHLVRPYVGIHLRIGSDWKNACAMLKDGTAGSHFMASPQCVGYSRSTATPLTMTMCLPDLKEIQRAVTLWVRALNARSVYIATDSESYVSEIQQLFKDKVRVVSLKPEVAQIDLYILGQADHFIGNCVSSFTAFVKRERDLHGRQSSFFGMDRPSQLRDEF

Catalytic domain sequence

350 aa

DLAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKPPFTNLHVSYQKYFKLEPLQAYHRVVSLEDFMENLAPSHWPPEKRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELFTGISFSASYKEQWTQRFPAKEHPVLALPGAPAQFPVLEEHRELQKYMVWSDEMVRTGEALISAHLVRPYVGIHLRIGSDWKNACAMLKDGTAGSHFMASPQCVGYSRSTATPLTMTMCLPDLKEIQRAVTLWVRALNARSVYIATDSESYVSEIQQLFKDKVRVVSLKPEVAQIDLYILGQADHFIGNCVSSFTAFVKRERDLHGRQSSFFGMD