GT-CORE (public beta v1)

Glycosyltransferase Curated Online Resource for Enzymology

Family-organised sequence, structure, mechanism and substrate-specificity records for the CAZy glycosyltransferases

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W0K5G2

Fusarium oxysporum f. sp. cubense · NEDD8-conjugating enzyme UBC12 (EC 2.3.2.23; 2.3.2.34; 2.4.1.16)

GT2Non-model organism · annotation 4–5Fold GT-AInverting

External resources

UniProt W0K5G2AlphaFold modelInterPro domainsCAZy GT2

Identification and annotation

CAZy familyGT2 · CAZy entry
Gene symbolnot curated
Synonyms / alternate names
  • NEDD8-conjugating enzyme Ubc12
  • RUB1-conjugating enzyme
  • Ubiquitin carrier protein 12
UniProt accessionW0K5G2
NCBI Gene IDnot curated
Source speciesFusarium oxysporum f. sp. cubense
Taxonomic domainEukaryota
UniProt annotation level4

Function and localisation

Enzyme functionNEDD8-conjugating enzyme UBC12 (EC 2.3.2.23; 2.3.2.34; 2.4.1.16)
Subcellular locationCell membrane

Structure and mechanism

Fold typeGT-A
Catalytic mechanismInverting
Cation dependenceNo
Oligomeric statenot curated
PDB structuresnot curated

Donor and acceptor specificity

Sugar nucleotide donorUDP-N-acetyl-alpha-D-glucosamine
Donor CCD code(s)UD1
Acceptor substrate(s)
  • [(1->4)-N-acetyl-beta-D-glucosaminyl](n)
  • [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
  • [E2 NEDD8-conjugating enzyme]-L-cysteine
Acceptor CCD / GlycoCTnot curated

Protein sequences

Full protein sequence

1394 aa 
MAYNGRDQEYGGHALQDLPAGGSQYHLPPQENDEEQGRGLLNSGYDQDRLGARTPPDRPVSAYSLTESYAPGASSTMPGQGPTGYGDGGSFGQFGNLDAAAPFPRPDSAFDPEDSWVERQQQPQMGGGLGRSKTRKIKLVQGSVLSIDYPVPSAIKNAVQPQYRDAESGTEEFHKMRYTAATCDPNDFTLKNGYDLRPRMYNRHTELLIAITYYNEDKVLLARTLHHTMQNIRDIVNLKKSTFWNKGGPAWQKIVVCLVFDGIDKADKNTLDVLATVGVYQDGVIKKDVDGKETVAHIFEYTSQLSVTPNQQLIRPTNEGSQNLPPVQMIFCLKQKNTKKINSHRWLFNAFGRILNPEVCILLDAGTKPSPRSLLALWEGFYNDKDLGGACGEIHAMLGKGGKKLFNPLVAVQNFEYKISNILDKPLESSFGYVSVLPGAFSAYRFRAIMGRPLEQYFHGDHTLSKMLGKKGIDGMNIFKKNMFLAEDRILCFELVAKAGQKWHLSYIKAAKGETDVPEGAAEFISQRRRWLNGSFAATLYSLMHFGRMYKSGHNIIRMFFLHIQLIYTTLNTLFAWFSLGSYWLTTSVIMDLVGTPKATSGQHAWPFGDTGTPVVNALLQYLYLAFVMLQFILALGNRPKGSKFTYIASFMVFSLIQGYILVLSAYLVVRAFDTPIGDQISFASTDAFLDSFFGGSSAGGVILVALITIYGLNFVASFMYLDPWHMFHSFVSSFPYYLLLMSTYINILMVYAFNNWHDVSWGTKGSDKAEALPSAHVTKGEKNEVVVEEVEKEQEDIDSQFEQTVRRALAPFKEEEEVEKADVEDGYKSFRTGLVVSWLFGNILLIVCITSDKFDNLGWGEPATERKAHYFQFLLYATAVLSLHDLILLATEFSVFTTNLSESCSATCPTLSYNLTFVSCLGSRTPFSVISSRVFPQRIATQPKPGPPTSTMLKIWSMKKEQKEAENAEGQATGGKKKKVTAAQLRVQKGMLHPVQTLTCSGVFRMKRLTHAATDLSELSLGSTMKTEFPDPDDILNFVLTIDPDEVEKEQEDIDSQFEQTVRRALAPFKEEEEVEKADVEDGYKSFRTGLVVSWLFGNILLIVCITSDKFDNLGWGEPATERKAHYFQFLLYATAVLSLHDLILLATEFSVFTTNLSESCSATCPTLSYNLTFVSCLGSRTPFSVISSRVFPQRIATQPKPGPPTSTMLKIWSMKKEQKEAENAEGQATGGKKKKVTAAQLRVQKDLSELSLGSTMKTEFPDPDDILNFVLTIDPDEGMYRGSRFTFDFTINQNFPHEPPKVRCREKIYHPNIDLEGKVCLNILREDWKPVLNLNAVIVGLQFLFLEPNASDPLNKEAAEDLRNNREGFKRNVRTAMGGGVVKGRTYDRVLK

Catalytic domain sequence

340 aa 
LLIAITYYNEDKVLLARTLHHTMQNIRDIVNLKKSTFWNKGGPAWQKIVVCLVFDGIDKADKNTLDVLATVGVYQDGVIKKDVDGKETVAHIFEYTSQLSVTPNQQLIRPTNEGSQNLPPVQMIFCLKQKNTKKINSHRWLFNAFGRILNPEVCILLDAGTKPSPRSLLALWEGFYNDKDLGGACGEIHAMLGKGGKKLFNPLVAVQNFEYKISNILDKPLESSFGYVSVLPGAFSAYRFRAIMGRPLEQYFHGDHTLSKMLGKKGIDGMNIFKKNMFLAEDRILCFELVAKAGQKWHLSYIKAAKGETDVPEGAAEFISQRRRWLNGSFAATLYSLMHF
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