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Glycosyltransferase Curated Online Resource for Enzymology

Family-organised sequence, structure, mechanism and substrate-specificity records for the CAZy glycosyltransferases

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Chsy3

Mus musculus · Chondroitin sulfate synthase 3 (EC 2.4.1.175; 2.4.1.226)

GT31Non-model organism · annotation 4–5Fold GT-AInverting

External resources

UniProt Q5DTK1AlphaFold modelInterPro domainsNCBI Gene 78923CAZy GT31

Identification and annotation

CAZy familyGT31 · CAZy entry
Gene symbolChsy3
Synonyms / alternate names
  • Chsy2
  • Css3
  • Kiaa4168
  • Carbohydrate synthase 2
  • Chondroitin glucuronyltransferase 3
  • Chondroitin synthase 2
  • Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II
  • N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 3
  • N-acetylgalactosaminyltransferase 3
UniProt accessionQ5DTK1
NCBI Gene ID78923
Source speciesMus musculus
Taxonomic domainEukaryota
UniProt annotation level5

Function and localisation

Enzyme functionChondroitin sulfate synthase 3 (EC 2.4.1.175; 2.4.1.226)
Subcellular location
  • Golgi apparatus
  • Golgi stack membrane

Structure and mechanism

Fold typeGT-A
Catalytic mechanismInverting
Cation dependenceYes (Mn2+)
Oligomeric stateForms heterodimers with CHSY1; this interaction is required
PDB structuresnot curated

Donor and acceptor specificity

Sugar nucleotide donor
  • UDP-N-acetyl-alpha-D-galactosamine
  • UDP-alpha-D-glucuronate
Donor CCD code(s)UD2, UGA
Acceptor substrate(s)
  • 3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein]
  • 3-O-{beta-D-GlcA-(1->3)-[beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)](n)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl}-L-seryl-[protein]
  • 3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein]
  • 3-O-{[beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)](n)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl}-L-seryl-[protein]
Acceptor CCD / GlycoCTnot curated

Protein sequences

Full protein sequence

884 aa 
MAVRSRRPWVSVALGLVLGFTAASWLIAPRVAELSEKRRRGSSLCSYYGRSATGPRADAQQLLPQPQSRPRLEQSPPPASHELPGPQQPEAAPGGPSFRSSPWQQPALLPQRRRGHTPEGATALPGAPAAKGEPEEEDGGAADPRKGGRPGSSHNGSGDGGAAVPTSGPGDFLYVGVMTAQKYLGSRALAAQRTWARFIPGRVEFFSSQQSPSAALGQPPPPLPVIALPGVDDSYPPQKKSFMMIKYMHDHYLDKYEWFMRADDDVYIKGDKLEEFLRSLNSSKPLYLGQTGLGNTEELGKLGLEPGENFCMGGPGMIFSREVLRRMVPHIGECLREMYTTHEDVEVGRCVRRFGGTQCVWSYEMQQLFHENYEHNRKGYIQDLHNSKIHAAITLHPNKRPAYQYRLHNYMLSRKISELRYRTIQLHRESALMSKLSNSEVSKEDQQLGRTPSFNHFQPRERNEVMEWEFLTGKLLYSAAENQPPRQSINSILRSALDDTVLQVMEMINENAKSRGRLIDFKEIQYGYRRVDPMHGVEYILDLLLLYKRHKGRKLTVPVRRHAYLQQPFSKPFFREVEELDVNRLVESINSGTQSFSVISNSLKILSSLQEAKDIGGHNEKKVHILVPLVGRYDIFLRFMENFESTCLIPKQNVKLVIILFSRDAGQESIKHIELIQEYQSRYPSAEMMLIPMKGEFSRGLGLEMASSQFDNDTLLLFCDVDLIFRGDFLQRCRDNTVQGQQVYYPIIFSQYDPKVTHMRNPPTEGDFVFSKETGFWRDYGYGITCIYKSDLLGAGGFDTSIQGWGLEDVDLYNKVILSGLRPFRSQEVGVVHIFHPVHCDPNLDPKQYKMCLGSKASTFASTMQLAELWLEKHLGVRDNRTLS

Catalytic domain sequence

537 aa 
HIGECLREMYTTHEDVEVGRCVRRFGGTQCVWSYEMQQLFHENYEHNRKGYIQDLHNSKIHAAITLHPNKRPAYQYRLHNYMLSRKISELRYRTIQLHRESALMSKLSNSEVSKEDQQLGRTPSFNHFQPRERNEVMEWEFLTGKLLYSAAENQPPRQSINSILRSALDDTVLQVMEMINENAKSRGRLIDFKEIQYGYRRVDPMHGVEYILDLLLLYKRHKGRKLTVPVRRHAYLQQPFSKPFFREVEELDVNRLVESINSGTQSFSVISNSLKILSSLQEAKDIGGHNEKKVHILVPLVGRYDIFLRFMENFESTCLIPKQNVKLVIILFSRDAGQESIKHIELIQEYQSRYPSAEMMLIPMKGEFSRGLGLEMASSQFDNDTLLLFCDVDLIFRGDFLQRCRDNTVQGQQVYYPIIFSQYDPKVTHMRNPPTEGDFVFSKETGFWRDYGYGITCIYKSDLLGAGGFDTSIQGWGLEDVDLYNKVILSGLRPFRSQEVGVVHIFHPVHCDPNLDPKQYKMCLGSKASTFASTMQL
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