A4gnt (GT32)

A4gnt

Mus musculus · Alpha-1,4-N-acetylglucosaminyltransferase (EC 2.4.1.-)

GT32Non-model organism · annotation 4–5Fold GT-ARetaining

External resources

UniProt Q14BT6 AlphaFold model InterPro domains NCBI Gene 333424 CAZy GT32

Identification and annotation

CAZy family	GT32 · CAZy entry
Gene symbol	A4gnt
Synonyms / alternate names	not curated
UniProt accession	`Q14BT6`
NCBI Gene ID	`333424`
Source species	Mus musculus
Taxonomic domain	Eukaryota
UniProt annotation level	5

Function and localisation

Enzyme function	Alpha-1,4-N-acetylglucosaminyltransferase (EC 2.4.1.-)
Subcellular location	Golgi apparatus membrane

Structure and mechanism

Fold type	GT-A
Catalytic mechanism	Retaining
Cation dependence	Yes (Mn2+)
Oligomeric state	not curated
PDB structures	not curated

Donor and acceptor specificity

Sugar nucleotide donor	UDP-N-acetyl-alpha-D-glucosamine 2 UDP-N-acetyl-alpha-D-glucosamine
Donor CCD code(s)	`UD1`
Acceptor substrate(s)	a beta-D-galactoside a 3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein] a 3-O-{beta-D-Gal-(1->3)-[beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->6)]-alpha-D-GalNAc}-L-threonyl-[protein] a 3-O-{beta-D-Gal-(1->3)-[beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->6)]-alpha-D-GalNAc}-L-seryl-[protein] 3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein]
Acceptor CCD / GlycoCT	not curated

Protein sequences

Full protein sequence

341 aa

MLKEIYLSLSLVLVFACGLLYQLTMRSQCFFACLPPFSFPQGLDGLLRSGRSIMFIETSERVEPPPMVSCAVESAAKIYPEQPIIFFMKGLRDSVQLTSNTSYPAFSLLSAINNVFFVPLDMERLFKDTPLFSWYTKVNSSTEKHWLHVSSDAARLAIIWKYGGIYMDTDVISLQPIPEENFLAAQGSRHSSNGVFGFLPHHPFLWACMENFVEHYDSTIWGNQGPQLMTRMLRVWCRLKDFHGLGDLKCLNISFLHPQRFYPIPYPQWKRYYQVWDKEPSFNESYALHLWNYMNKEGKTVVRGSKTLVENLYQKHCPKTYRVLIQGAEGTVSKKPGTGSR

Catalytic domain sequence

126 aa

FLPHHPFLWACMENFVEHYDSTIWGNQGPQLMTRMLRVWCRLKDFHGLGDLKCLNISFLHPQRFYPIPYPQWKRYYQVWDKEPSFNESYALHLWNYMNKEGKTVVRGSKTLVENLYQKHCPKTYRV