Home/GT Families/GT44/tcsL
tcsL
Paraclostridium sordellii · Cytotoxin-L (EC 3.4.22.-)
GT44Non-model organism · annotation 4–5Fold GT-ARetaining1 PDB structure(s)
External resources
Identification and annotation
| CAZy family | GT44 · CAZy entry |
|---|
| Gene symbol | tcsL |
|---|
| Synonyms / alternate names | Lethal toxin |
|---|
| UniProt accession | P0DUB4 |
|---|
| NCBI Gene ID | not curated |
|---|
| Source species | Paraclostridium sordellii |
|---|
| Taxonomic domain | Bacteria |
|---|
| UniProt annotation level | 5 |
|---|
Function and localisation
| Enzyme function | Cytotoxin-L (EC 3.4.22.-) |
|---|
| Subcellular location | not curated |
|---|
Structure and mechanism
| Fold type | GT-A |
|---|
| Catalytic mechanism | Retaining |
|---|
| Cation dependence | Yes (Mn2+, Mg2+) |
|---|
| Oligomeric state | Homomultimer; forms an inactive homomultimer at pH 8, which |
|---|
| PDB structures | 6WTS |
|---|
Donor and acceptor specificity
| Sugar nucleotide donor | UDP-alpha-D-glucose |
|---|
| Donor CCD code(s) | UPG |
|---|
| Acceptor substrate(s) | L-threonyl-[protein] |
|---|
| Acceptor CCD / GlycoCT | not curated |
|---|
Protein sequences
Full protein sequence
2364 aa
MSLVNKAQLQKMAYVKFRIQEDEYVAILNALEEYHNMSESSVVEKYLKLKDINNLTDNYLNTYKKSGRNKALKKFKEYLTMEVLELKNNSLTPVEKNLHFIWIGGQINDTAINYINQWKDVNSDYTVKVFYDSNAFLINTLKKTIVESATNNTLESFRENLNDPEFDYNKFYRKRMEIIYDKQKHFIDYYKSQIEENPEFIIDNIIKTYLSNEYSKDLEALNKYIEESLNKITANNGNDIRNLEKFADEDLVRLYNQELVERWNLAAASDILRISMLKEDGGVYLDVDMLPGIQPDLFKSINKPDSITDTSWEMIKLEAIMKYKEYIPGYTSKNFDMLDEEVQSSFESALSSTSDKSEIFLPLDDIKVSPLEVKIAFANNSVINQALISLKDSYGSDLVISQIKNRYKILNDNLNPAINEGNDFNTTMKTFNDNLVSISNEDNIMFMIKIADYLKVGFAPDVRSTINLSGPGVYTGAYQDLLMFKDNSINIHLLEPELRNFEFPKTKISQLTEQEITSLWSFNQARAKSQFEEYKKGYFEGALGEDDILDFSQNTVLDKDYVVEKISSSMRTPNKEYVHYIVQLQGDNVSYEAACNLFAKNPYYNILFQKNIENSETAYYYNLIYNKLQEIDKYRIPNLISNRHKIKLTFIGHGKSEFNTDTFANLDVNSLSSEIETILNLAKEDISPKSIEINLLGCNMFSYNVNVEETYPGKLLLKIKDIVSKLMPSISQDSITVSANQYEVRINKEGRRELLDHSGKWINKEESIIKDISSKEYISFNPKENKIIVKSKNLHELSTLLQEIKNNSNSSDIDLEKKVMLTECEINVASNIDTQIVEERIEEAKNLTSDSINYIKNEFKLIESISDALYDLKHQNGLDDSHFISFEDISKTENGFRIRFINKETGNSIFIETEKEIFSEYAAHISKEISNIKDTIFDNVNGKLVKKVNLDAAHEVNTLNSAFFIQSLIEYNTTKESLSNLSVAMKVQVYAQLFSTGLNTITDASKVVELVSTALDETIDLLPTLSEGLPIIATIIDGVSLGAAIKELSETNDPLLRQEIEAKIGIMAVNLTAASTAIVTSALGIASGFSILLVPLAGISAGIPSLVNNELILQDKATKVIDYFKHISLAETEGAFTLLDDKIIMPQDDLVLSEIDFNNNSITLGKCEIWRTEGGSGHTFTDDIDHFFSSPSITYRKPWLSIYDVLNIKKEKIDFSKDLMVLPNAPNRVFSYEMGWTPGFRSLDNDGTKLLDRIRDHYEGQFYWRYFAFIADALITKLKPRYEDTNVRINLDGNTRSFIVPVITTEQIRKNLSYSFYGSGGSYSLSLSPYNMNIDLNLVENDTWVIDVDNVVKNITIESDEIQKGELIENILSKLNIEDNKIVLNNHTINFYGAINESNRFISLTFSILEDINIIIEIDLVSKSYKILLSGNCIKLIENSSDIQQKIDHIGFNGEHQKYIPYSYIDNETKYNGFIDYSKKEGLFTAEFSNESIIRNIYMPDSNNLFIYSSKDLKDIRIINKGDVKLLIGNYFKDNMKVSLSFTIEDTNTIKLNGVYLDENGVAQILKFMNNAKSALNTSNSLMNFLESINIKNIFYNNLDPNIKFILDTNFIISGSNSIGQFELICDKDKNIQPYFIKFKIKETSYTLYAGNRQNLIVEPSYHLDDSGNISSTVINFSQKYLYGIDRYVNKVIITPNLYTDEINITPVYKPNYICPEVIILDANYINEKINININDLSIRYVWDNDGSDLILIANSEEDNQPQVKIRFVNVFKSDTAADKLSFNFSDKQDVSVSKIISTFSLAAYSDGVFDYEFGLVSLDNECFYINSFGNMVSGLIYINDSLYYFKPPKNNLITGFTTIDDNKYYFDPTKSGAASIGEITIDGKDYYFNKQGILQVGVINTSDGLKYFAPAGTLDENLEGESVNFIGKLNIDGKIYYFEDNYRAAVEWKSLDGETYYFNPKTGEALKGLHQIGDNKYYFDNNGIMQTGFITINDKVFYFNNDGVMQVGYIEVNGKYFYFGKNGERQLGVFNTPDGFKFFGPKDDDLGTEEGELTLYNGILNFNGKIYFFDISNTAVVGWGILDDGSTYYFDDNTAEACIGLTVINDCKYYFDDNGIRQLGFITINDNIFYFSESGKIELGYQNINGNYFYIDESGLVLIGVFDTPDGYKYFAPLNTVNDNIYGQAVEYSGLVRLNEDVYYFGETYKIETGWIENETDKYYFDPETKKAYKGINVVDDIKYYFDENGIMKTGLISFENNNYYFNEDGKMQFGYLNIKDKMFYFGKDGKMQIGVFNTPDGFKYFAHQNTLDENFEGESINYTGWLDLDGKRYYFTDEYIAATSSLTIDGYNYYFDPDTAELVVSE
Catalytic domain sequence
373 aa
KNLHFIWIGGQINDTAINYINQWKDVNSDYTVKVFYDSNAFLINTLKKTIVESATNNTLESFRENLNDPEFDYNKFYRKRMEIIYDKQKHFIDYYKSQIEENPEFIIDNIIKTYLSNEYSKDLEALNKYIEESLNKITANNGNDIRNLEKFADEDLVRLYNQELVERWNLAAASDILRISMLKEDGGVYLDVDMLPGIQPDLFKSINKPDSITDTSWEMIKLEAIMKYKEYIPGYTSKNFDMLDEEVQSSFESALSSTSDKSEIFLPLDDIKVSPLEVKIAFANNSVINQALISLKDSYGSDLVISQIKNRYKILNDNLNPAINEGNDFNTTMKTFNDNLVSISNEDNIMFMIKIADYLKVGFAPDVRSTINL
