GT-CORE (public beta v1)

Glycosyltransferase Curated Online Resource for Enzymology

Family-organised sequence, structure, mechanism and substrate-specificity records for the CAZy glycosyltransferases

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B4galt4

Rattus norvegicus · Beta-1,4-galactosyltransferase 4 (EC 2.4.1.-; 2.4.1.275; 2.4.1.90)

GT7Non-model organism · annotation 4–5Fold GT-AInverting

External resources

UniProt Q66HH1AlphaFold modelInterPro domainsNCBI Gene 303923CAZy GT7

Identification and annotation

CAZy familyGT7 · CAZy entry
Gene symbolB4galt4
Synonyms / alternate names
  • Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
  • Lactotriaosylceramide beta-1,4-galactosyltransferase
  • N-acetyllactosamine synthase
  • Nal synthase
  • UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
  • UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
UniProt accessionQ66HH1
NCBI Gene ID303923
Source speciesRattus norvegicus
Taxonomic domainEukaryota
UniProt annotation level5

Function and localisation

Enzyme functionBeta-1,4-galactosyltransferase 4 (EC 2.4.1.-; 2.4.1.275; 2.4.1.90)
Subcellular location
  • Golgi apparatus membrane
  • Secreted

Structure and mechanism

Fold typeGT-A
Catalytic mechanismInverting
Cation dependenceYes (Mn2+)
Oligomeric stateInteracts with SLC35A2/UGT1
PDB structuresnot curated

Donor and acceptor specificity

Sugar nucleotide donor
  • UDP-alpha-D-galactose
  • 2 UDP-alpha-D-galactose
Donor CCD code(s)GDU
Acceptor substrate(s)
  • N-acetyl-D-glucosamine
  • a beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-Cer(d18:1(4E))
  • 3-O-{beta-D-galactosyl-(1->3)-[6-O-sulfo-N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein]
  • 3-O-{beta-D-galactosyl-(1->3)-[6-O-sulfo-N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein]
  • an N(4)-{6-O-sulfo-beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L--asparaginyl-[protein]
Acceptor CCD / GlycoCTnot curated

Protein sequences

Full protein sequence

344 aa 
MGCNPPYLLSYRLRLLLLFTLCLTVLGWATSNYFVGAIQVIPRAKNFMATLHKVMYLGNEETLGHGAAMKKAELANCPSVSPNLRGQNKLVFKPDLTLEEVQAKNPKVSRGRYRPEECKALQRVAVLIPHRNREKHLIYLLEHLHPFLQRQQLDYGIYVIHQTGSKKFNRAKLLNVGYLEALKEENWDCFIFHDVDLVPENDFNLYTCGDQPKHLVVGRNSTGYRLRYSKYFGGVTALSREQFFKVNGFSNNYWGWGGEDDDLRLRVELHKMKISRPKPDVGKYTMIFHTRDKGNEVNGSRMKLLQQMSRVWKTDGLSSCSYRLLSVEHNPLYANITVDFWTAA

Catalytic domain sequence

218 aa 
QRVAVLIPHRNREKHLIYLLEHLHPFLQRQQLDYGIYVIHQTGSKKFNRAKLLNVGYLEALKEENWDCFIFHDVDLVPENDFNLYTCGDQPKHLVVGRNSTGYRLRYSKYFGGVTALSREQFFKVNGFSNNYWGWGGEDDDLRLRVELHKMKISRPKPDVGKYTMIFHTRDKGNEVNGSRMKLLQQMSRVWKTDGLSSCSYRLLSVEHNPLYANITVD
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