waaO (GT8)

waaO

Escherichia coli (strain K12) · Lipopolysaccharide glucosyltransferase WaaO (EC 2.4.1.73)

GT8Model organism · annotation ≥ 4Fold GT-ARetaining

External resources

UniProt P27128 AlphaFold model InterPro domains NCBI Gene 948143 CAZy GT8

Identification and annotation

CAZy family	GT8 · CAZy entry
Gene symbol	waaO
Synonyms / alternate names	rfaI Lipopolysaccharide alpha-1,3 glucosyltransferase Lipopolysaccharide glucosyltransferase II
UniProt accession	`P27128`
NCBI Gene ID	`948143`
Source species	Escherichia coli (strain K12)
Taxonomic domain	Bacteria
UniProt annotation level	5

Function and localisation

Enzyme function	Lipopolysaccharide glucosyltransferase WaaO (EC 2.4.1.73)
Subcellular location	not curated

Structure and mechanism

Fold type	GT-A
Catalytic mechanism	Retaining
Cation dependence	Yes (Mg2+)
Oligomeric state	not curated
PDB structures	not curated

Donor and acceptor specificity

Sugar nucleotide donor	UDP-glucose UDP-alpha-D-glucose
Donor CCD code(s)	`UPG`
Acceptor substrate(s)	lipopolysaccharide alpha-D-Gal-(1->6)-alpha-D-Glc-(1->3)-[L-alpha-D-Hep-(1->7)]-4-O-PO3(2-)-L-alpha-D-Hep-(1->3)-4-O-PO3(2-)-L-alpha-D-Hep-(1->5)-[alpha-Kdo-(2->4)]-alpha-Kdo-(2->6)-lipid A
Acceptor CCD / GlycoCT	not curated

Protein sequences

Full protein sequence

339 aa

MQQVFFQETEFLNSVIDYDHKVETENLCLDIAYGTDKNFLFGCGISIASILKYNEGSRLCFHIFTDYFGDDDRKYFDALALQYKTRIKIYLINGDRLRSLPSTKNWTHAIYFRFVIADYFINKAPKVLYLDADIICQGTIEPLINFSFPDDKVAMVVTEGQADWWEKRAHSLGVAGIAKGYFNSGFLLINTAQWAAQQVSARAIAMLNEPEIIKKITHPDQDVLNMLLADKLIFADIKYNTQFSLNYQLKESFINPVTNDTIFIHYIGPTKPWHDWAWDYPVSQAFMEAKNASPWKNTALLKPNNSNQLRYSAKHMLKKHRYLKGFSNYLFYFIEKIKH

Catalytic domain sequence

247 aa

IAYGTDKNFLFGCGISIASILKYNEGSRLCFHIFTDYFGDDDRKYFDALALQYKTRIKIYLINGDRLRSLPSTKNWTHAIYFRFVIADYFINKAPKVLYLDADIICQGTIEPLINFSFPDDKVAMVVTEGQADWWEKRAHSLGVAGIAKGYFNSGFLLINTAQWAAQQVSARAIAMLNEPEIIKKITHPDQDVLNMLLADKLIFADIKYNTQFSLNYQLKESFINPVTNDTIFIHYIGPTKPWHDWA