GT-CORE (public beta v1)

Glycosyltransferase Curated Online Resource for Enzymology

Family-organised sequence, structure, mechanism and substrate-specificity records for the CAZy glycosyltransferases

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B3GNT3

Homo sapiens · N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3 (EC 2.4.1.149; 2.4.1.146)

GT31Model organism · annotation ≥ 4Fold GT-AInverting

External resources

UniProt Q9Y2A9AlphaFold modelInterPro domainsNCBI Gene 10331CAZy GT31

Identification and annotation

CAZy familyGT31 · CAZy entry
Gene symbolB3GNT3
Synonyms / alternate names
  • B3GALT8
  • TMEM3
  • Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase
  • Beta-1,3-galactosyltransferase 8
  • Beta-3-Gx-T8
  • Core 1 extending beta-1,3-N-acetylglucosaminyltransferase
  • Core1-beta3GlcNAcT
  • Transmembrane protein 3
  • UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 8
  • UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3
  • UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 8
UniProt accessionQ9Y2A9
NCBI Gene ID10331
Source speciesHomo sapiens
Taxonomic domainEukaryota
UniProt annotation level5

Function and localisation

Enzyme functionN-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3 (EC 2.4.1.149; 2.4.1.146)
Subcellular locationGolgi apparatus membrane

Structure and mechanism

Fold typeGT-A
Catalytic mechanismInverting
Cation dependenceYes (Mn2+)
Oligomeric statenot curated
PDB structuresnot curated

Donor and acceptor specificity

Sugar nucleotide donorUDP-N-acetyl-alpha-D-glucosamine
Donor CCD code(s)UD1
Acceptor substrate(s)
  • a 3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-threonyl-[protein]
  • 3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl-[protein]
  • a beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative
Acceptor CCD / GlycoCTnot curated

Protein sequences

Full protein sequence

372 aa 
MKYLRHRRPNATLILAIGAFTLLLFSLLVSPPTCKVQEQPPAIPEALAWPTPPTRPAPAPCHANTSMVTHPDFATQPQHVQNFLLYRHCRHFPLLQDVPPSKCAQPVFLLLVIKSSPSNYVRRELLRRTWGRERKVRGLQLRLLFLVGTASNPHEARKVNRLLELEAQTHGDILQWDFHDSFFNLTLKQVLFLQWQETRCANASFVLNGDDDVFAHTDNMVFYLQDHDPGRHLFVGQLIQNVGPIRAFWSKYYVPEVVTQNERYPPYCGGGGFLLSRFTAAALRRAAHVLDIFPIDDVFLGMCLELEGLKPASHSGIRTSGVRAPSQRLSSFDPCFYRDLLLVHRFLPYEMLLMWDALNQPNLTCGNQTQIY

Catalytic domain sequence

189 aa 
RRELLRRTWGRERKVRGLQLRLLFLVGTASNPHEARKVNRLLELEAQTHGDILQWDFHDSFFNLTLKQVLFLQWQETRCANASFVLNGDDDVFAHTDNMVFYLQDHDPGRHLFVGQLIQNVGPIRAFWSKYYVPEVVTQNERYPPYCGGGGFLLSRFTAAALRRAAHVLDIFPIDDVFLGMCLELEGLK
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