Glyco-Enzyme Repository

Repository of Expression Constructs for Glycosylation Enzymes

Gateway® entry clones and mammalian, baculovirus and bacterial expression constructs for glycosyltransferases, glycoside hydrolases and glycan-modifying enzymes

Downloads

Machine-readable exports of the record set, the original expression-summary spreadsheets, and the vector sequence files. The 1870 per-construct files — annotated GenBank sequences, vector maps and sequence PDFs — are linked from each gene record rather than listed here.

Record exports

Generated from the same dataset that builds the pages, so they cannot drift out of step with the site.

Expression summary spreadsheets

These are the original workbooks from the legacy site, unmodified. They carry the measured expression levels for the constructs, which the individual gene records do not.

Destination vector sequences

VectorSystemFiles
pGEn1-DESTMammalian
pGEn2-DESTMammalian
pGEn3-DESTMammalian
pGEc1-DESTMammalian
pGEc2-DESTMammalian
Ac-polh-CtermHisStrep-DESTBaculovirus
Ac-polh-NtermMelHisStrep-DESTBaculovirus
Ac-polh-NtermMelHisAviTagGFP-DESTBaculovirus
pET16-DESTBacterial
pET32-DESTBacterial

Protocols

Column definitions for the CSV exports

ColumnMeaning
gene_symbolGene symbol as recorded, including any parenthesised legacy synonym.
symbolCurrent gene symbol with the synonym stripped.
synonymsLegacy synonyms, semicolon-separated.
enzyme_classGT, GH, Other or Bacterial.
cazy_familyCAZy family label(s) parsed from the record; blank when the enzyme is not CAZy-classified.
uniprotUniProt accession.
gene_idNCBI GeneID.
dna_refseqRefSeq nucleotide accession.
protein_refseqRefSeq protein accession.
mgc_accMammalian Gene Collection accession of the PCR template.
protein_nameProtein name or GenBank annotation as recorded.
speciesSource species; populated for the bacterial homologues.
domain_structureMembrane topology class of the native enzyme.
truncation_eukaryoticTruncation applied for eukaryotic hosts.
truncation_bacterialTruncation applied for the bacterial host.
n_constructsNumber of construct entries on the record.
n_sequence_filesNumber of linked GenBank/map/sequence files.
dnasu_clone_idsAll DNASU clone IDs on the record, semicolon-separated.
availabilitydnasu, jarvis or pending — see the availability key on any family page.
record_pageRelative path to the record page in this site.

Known gaps in the file set

36 construct file references on the original site pointed at files that server did not hold — requesting them returned 404, so there is nothing to migrate. Those references are kept visible on the affected records, marked unavailable, because the reference is itself evidence the construct was built; they are excluded from the CSV and JSON exports, which list only files that exist. Every other referenced file is present in this site.

The construct export (all_constructs.csv) has one row per construct rather than per gene, and adds construct_kind (entry clone or expression construct), host, vector, fusion_strategy, dnasu_clone_id and files — the site-relative paths of that construct's sequence files, semicolon-separated.